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Hormone-free tissue culture method for dendrobium officinale

A Dendrobium officinale, tissue culture technology, applied in the field of plant tissue culture, to achieve the effect of simple operation steps, reduce production costs, and maintain excellent traits

Inactive Publication Date: 2014-10-01
GUANGZHOU HUADU PIONEER HORTICULTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method does not use hormones for cultivation, and will not interfere with the growth of Dendrobium candidum, but there are too many types of culture media, and the cultivated Dendrobium candidum still has certain deficiencies in nutrition and medicinal components.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The hormone-free tissue culture method for Dendrobium officinale comprises the following steps:

[0029] a. Seed induction and cultivation: Wipe the surface of the picked Dendrobium officinale pods with 75% alcohol, and then soak them in 1% sodium hypochlorite aqueous solution for 15 minutes; then, on the ultra-clean workbench, use a sterile knife to cut Dendrobium officinale Fruit pods, the seeds in the fruit pods are clipped and spread on a sterile medium to induce seed germination;

[0030] Then, place the sterile medium on the culture rack, and cultivate for 90 days under the conditions of 12h / d illumination time, 22-26°C culture temperature, and 1500lx illumination intensity, and the seeds germinate to form protocorms;

[0031] Described sterile medium is specifically: MS medium+white granulated sugar 30g / L+agar 6g / L+potato 100g / L, pH is 6.4;

[0032] b. Protocorm proliferation: transfer the protocorm obtained in step a to the proliferation medium for cultivation,...

Embodiment 2

[0037] The hormone-free tissue culture method for Dendrobium officinale comprises the following steps:

[0038] a. Seed induction and cultivation: Wipe the surface of the picked Dendrobium officinale pods with 75% alcohol, and then soak them in 1% sodium hypochlorite aqueous solution for 15 minutes; then, on the ultra-clean workbench, use a sterile knife to cut Dendrobium officinale. Fruit pods, the seeds in the fruit pods are clipped and spread on a sterile medium to induce seed germination;

[0039] Then, place the sterile medium on the culture rack, and cultivate for 90 days under the conditions of 12h / d illumination time, 20-22°C culture temperature, and 1500lx illumination intensity, and the seeds germinate to form protocorms;

[0040] Described sterile medium is specifically: MS medium+white granulated sugar 30g / L+agar 6g / L+potato 100g / L, pH is 6.4;

[0041] b. Protocorm proliferation: transfer the protocorm obtained in step a to the proliferation medium for cultivation...

Embodiment 3

[0046] The hormone-free tissue culture method for Dendrobium officinale comprises the following steps:

[0047] a. Seed induction and cultivation: Wipe the surface of the picked Dendrobium officinale pods with 75% alcohol, and then soak them in 1% sodium hypochlorite aqueous solution for 15 minutes; then, on the ultra-clean workbench, use a sterile knife to cut Dendrobium officinale Fruit pods, the seeds in the fruit pods are clipped and spread on a sterile medium to induce seed germination;

[0048] Then, the sterile medium was placed on the culture rack, and cultivated for 90 days under the conditions of 12h / d light time, 26-30°C culture temperature, and 1500lx light intensity, and the seeds germinated to form protocorms;

[0049] Described sterile medium is specifically: MS medium+white granulated sugar 30g / L+agar 6g / L+potato 100g / L, pH is 6.4;

[0050] b. Protocorm proliferation: transfer the protocorm obtained in step a to the proliferation medium for cultivation, and th...

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PUM

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Abstract

The invention relates to a hormone-free tissue culture method for dendrobium officinale. The hormone-free tissue culture method comprises the following steps: a, inducing and culturing seeds; b, proliferating protocorms; c, rooting proliferated seedlings. In the step a and the step b, an adopted culture medium contains an MS (Murashige and Skoog) culture medium, 30g / L of white granulated sugar, 6g / L of agar and 100g / L of potatoes and the pH (Potential of Hydrogen) is 5.8-6.5; a culture medium adopted by the step c contains the MS culture medium, 30g / L of the white granulated sugar, 6g / L of the agar, 100g / L of the potatoes and 5g / L of active carbon, and the pH (Potential of Hydrogen) is 5.8-6.5. The method provided by the invention is simple and convenient in operation steps, and short in production period; the manpower and the material resources are saved; culture conditions are simple, the management is convenient and the space is saved; the types of the used culture mediums are few; the method is simple in preparation, reduces the production cost and is safe and environment-friendly; the cultured dendrobium officinale has high nutrition and medicinal component contents and better medicinal effects.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a hormone-free tissue culture method for Dendrobium officinale. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo), also known as: black knotweed, Yunnan iron. It is a perennial epiphytic herb of Orchidaceae. Stems erect, cylindrical, 9-35 cm long, 2-4 mm thick, yellow-green sepals and petals, nearly similar, oblong-lanceolate, about 1.8 cm long, 4-5 mm wide, flowering from March to June. Mainly distributed in Anhui, Zhejiang, Fujian and other places in China. Its stem is used as medicine, which belongs to the yin-tonifying medicine in the tonic medicine: Yiweishengjin, nourishing yin and clearing heat. Dendrobium candidum has high medicinal value. The existing wild Dendrobium candidum resources are scarce, and the traditional methods of cuttings and divisions have low reproduction rates. In order to solve this proble...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李虬
Owner GUANGZHOU HUADU PIONEER HORTICULTURE
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