Preparation method and application of modified hydroxyapatite microcapsule biological material carrier

A technology of hydroxyapatite and biological materials, which is applied in the directions of non-active ingredients, such as medical preparations, drug delivery, and pharmaceutical formulations, can solve the problems of low carrying capacity, easy dissipation, and difficult control of the release process, and achieves low cost. , type and dosage are small, the effect of active ingredient isolation

Active Publication Date: 2014-10-01
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when nanoparticles are directly used to load biological materials, there are disadvantages such as relatively small loading ...

Method used

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  • Preparation method and application of modified hydroxyapatite microcapsule biological material carrier
  • Preparation method and application of modified hydroxyapatite microcapsule biological material carrier
  • Preparation method and application of modified hydroxyapatite microcapsule biological material carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 (product preparation):

[0051] A modified hydroxyapatite microcapsule biological material carrier, the preparation steps of which are as follows:

[0052] (1) Modification of hydroxyapatite nanoparticles:

[0053] 0.500g (4.98×10 -4 mol) hydroxyapatite nanoparticles were placed in a round bottom flask, then 0.5mmol of dibutyl phosphate was added, and then 50ml of acetone was added to disperse to form a reaction system. The concentration of dibutyl phosphate in the reaction system was 0.01mol / L. Under the condition of 57°C water bath, stir and reflux for 5 hours; after the reflux, the product is separated by suction filtration, and then the product is washed 8 times with acetone by suction filtration; the washed product nanoparticles are collected and dried in a vacuum oven at 60°C for 24 Hour.

[0054] (2) Preparation of modified hydroxyapatite microcapsule biomaterial carrier:

[0055] Put 20 mg of the dried product nanoparticles obtained in step (1) ...

Embodiment 2

[0056] Embodiment 2 (product preparation):

[0057] A modified hydroxyapatite microcapsule biological material carrier, the preparation steps of which are as follows:

[0058] (1) Modification of hydroxyapatite nanoparticles:

[0059] 0.500g (4.98×10 -4 mol) hydroxyapatite nanoparticles were placed in a round bottom flask, then 0.5mmol of dibutyl phosphate was added, and then 50ml of acetone was added to disperse to form a reaction system. The concentration of dibutyl phosphate in the reaction system was 0.01mol / L. Under the condition of a water bath at 57°C, stir and reflux for 5 hours; after the reflux, separate the product by suction filtration, and then wash the product 8 times with acetone by suction filtration. The product nanoparticles after washing were collected and dried in a vacuum oven at 60° C. for 24 hours.

[0060] (2) Preparation of modified hydroxyapatite microcapsule biomaterial carrier:

[0061] Put 20 mg of the dried product nanoparticles obtained in st...

Embodiment 3

[0062] Embodiment 3 (application): hydroxyapatite microcapsule material is used as the carrier application experiment of fluorescent dye calcein

[0063] Choose to use 10 -5 The mol / l calcein physiological saline solution is used as the water phase, and the edible oil is used as the oil phase. Calcein is a small molecule water-soluble fluorescein with stable properties and can be easily detected by fluorescence spectrophotometry or ultraviolet-visible spectrophotometry. It is often used as a model drug for drug carriers.

[0064] Use 1ml of edible oil as the oil phase, add 20 mg of the modified hydroxyapatite nanoparticles prepared in step (1) of Example 1, use ultrasonic waves to fully disperse it in the oil phase, and then add 100 μl of 10 -5 mol / l calcein saline solution, shake vigorously to form emulsion. Observation with an optical microscope: it can be seen that the microcapsule material is formed with calcein saline solution as the core material and modified hydroxyap...

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Abstract

The invention belongs to the technical field of preparation and application of biocompatibility carrier materials and specifically discloses a preparation method and application of a modified hydroxyapatite microcapsule biological material carrier. The preparation method of the modified hydroxyapatite microcapsule biological material carrier comprises the following steps: adding hydroxyapatite nano-particles and dibutyl phosphate in acetone, modifying the surfaces of the nano-particles by using n-butyl, simultaneously generating hydroxyl groups and n-butyl groups on the surfaces of the nano-particles so as to obtain nano-particles with hydrophilcity/hydrophobicity and the modified surfaces; adding the nano-particles in a water phase or an oil phase, then adding the oil phase or the water phase to be used as a core material, violently shaking and carrying out self-assembling to obtain the microcapsule material which takes the nano-particles with modified surfaces as membrane materials and has water/oil interface stability. The microcapsule material has a relatively large loading rate, the function of isolating effective components and has the specific release capability and the like; by dissolving single water-soluble or fat-soluble biological material in the core material, the microcapsule carrier material can be conveniently taken as an efficient carrier of the biological material for practical application.

Description

technical field [0001] The invention relates to the technical field of preparation and application of biocompatible carrier materials, in particular to the preparation and application of a modified hydroxyapatite microcapsule biomaterial carrier. Background technique [0002] In recent years, the field of biomaterials has developed rapidly, and the research and application of inorganic biomedical materials are very active. Among them, the research and clinical application of hydroxyapatite (HA or HAP) active ceramic materials have attracted much attention. application. Hydroxyapatite is a surface-active material, which has similar chemical composition and structure to biological hard tissues, so it has good biological activity and compatibility. Internationally, a variety of hydroxyapatite composite materials, coating materials and nanomaterials have been developed by using different processes, and breakthroughs have been made. The development of functional hydroxyapatite c...

Claims

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Application Information

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IPC IPC(8): A61K47/24A61K47/04A61K9/52
Inventor 孙杰尹杉杉张玉峰张泽会李玫
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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