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Preparation method for typhonium giganteum polysaccharide extract

A technology of unicorn lotus polysaccharides and extracts, which is applied in the direction of drug combinations, anti-toxic agents, anti-tumor drugs, etc., can solve the problems of low yield of active ingredient extracts, high energy consumption, long extraction time, etc., and achieve significant anti-caseation Amino acid kinase, high extraction rate, and the effect of saving extraction time

Active Publication Date: 2014-10-01
SHENYANG HONGQI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The paper published by the applicant of this patent introduces the purification method of extracting unicorn lotus polysaccharide by water extraction and alcohol precipitation method, and removing protein by Sevag method, but this extraction method has long extraction time, high energy consumption, and low yield of active ingredient extract, etc. question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Add the compound enzyme (0.5g papain, 0.5g cellulase, and 0.5g amylase) to 50g dry rhizome powder of unicorn lotus, add 200ml of water, in a 25KHz ultrasonic generator, at 30 ℃, ultrasonic Enzyme hydrolysis for 2 hours, after the end of the enzymatic hydrolysis, inactivate the enzyme at 100°C for 1 hour. Select the ultrasonic condition with a frequency of 100KHz to extract the above-mentioned pretreated extraction material twice, each time for 1 hour, combine the extraction solution, and centrifuge it for 10 minutes under the condition of 3000 rpm in a centrifuge, and solidify the treated material. Concentrate the supernatant after discarding the solids; Concentrate the collected supernatant under reduced pressure at 60°C to 1 / 5 of the original volume; Stir the concentrated solution with 95% ethanol After mixing, the concentrated solution was alcohol-precipitated for 12 hours, centrifuged at 3000 rpm for 10 minutes, and the polysaccharides at the bottom of the centrifug...

Embodiment 2

[0022] Add compound enzymes (1.0g cellulase and 0.5g pectinase) to 50g dried unicorn rhizome powder, add 250ml of water, and ultrasonically hydrolyze for 2 hours at 30°C in a 25KHz ultrasonic generator. After the enzymatic hydrolysis, inactivate the enzyme at 100°C for 1 h. Select the ultrasonic condition with a frequency of 80KHz to extract the above-mentioned pretreated extraction materials twice, each time for 1 hour, combine the extracts, and centrifuge them under the condition of 3000 rpm for 10 minutes in a centrifuge, and solidify the treated materials. Concentrate the supernatant after discarding the solids; Concentrate the collected supernatant under reduced pressure at 60°C until it is concentrated to 1 / 4 of the original volume; Stir the concentrated solution with 80% ethanol After mixing, the concentrated solution was alcohol-precipitated for 10 hours, centrifuged at 3000 rpm for 10 minutes, and the polysaccharides at the bottom of the centrifuge cup were collected;...

Embodiment 3

[0024] Add compound enzyme (1.0g cellulase, and 0.5g amylase) to 50g dry rhizome powder of unicorn lotus, add water 500ml, in 25KHz ultrasonic generator, 30℃, ultrasonic enzymolysis 4h, enzyme After the digestion, the enzyme was inactivated at 100°C for 1 h. Select the ultrasonic condition with a frequency of 100KHz to extract the above-mentioned pretreated extraction material twice, each time for 1 hour, combine the extraction solution, and centrifuge it for 10 minutes under the condition of 3000 rpm in a centrifuge, and solidify the treated material. Concentrate the supernatant after discarding the solids; Concentrate the collected supernatant under reduced pressure at 60°C until it is concentrated to 1 / 4 of the original volume; Stir the concentrated solution with 90% ethanol After mixing, the concentrated solution was alcohol-precipitated for 12 hours, centrifuged at 3000 rpm for 10 minutes, and the polysaccharides at the bottom of the centrifuge cup were collected; the col...

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Abstract

The invention discloses a preparation method for typhonium giganteum polysaccharide extract, and relates to a preparation method for polysaccharide extract. The preparation method specifically comprises the following preparation steps of: adding 1-10% (in percent by weight) of compound enzyme and 3-15% of water into dried typhonium giganteum rhizome powder for carrying out enzymolysis under an ultrasonic condition; carrying out extraction / suction filtration under the ultrasonic condition; concentrating by a centrifugal machine, adopting 70-95% alcohol to stir and mix the concentrated liquor, carrying out alcohol precipitation for 6-18 hours, centrifuging for 5-20 minutes under rotation speed of 3000 revolutions / minute, collecting polysaccharides on the bottom of a centrifugal cup; and drying to obtain typhonium giganteum polysaccharide extract. The preparation method disclosed by the invention is simple and practical in operation, is high in extracting efficiency, and time for extracting typhonium giganteum polysaccharide by applying the process can be saved; and the extract has remarkable biological activities for preventing tyrosine kinase, oxidization, tumors, and the like.

Description

technical field [0001] The invention relates to a preparation method of a polysaccharide extract, in particular to a preparation method of a unicorn lotus polysaccharide extract. Background technique [0002] The unicorn lotus (Typhonium giganteum Engl.) is a plant belonging to the genus Typhonium Schott of the family Araceae, and it is a unique plant in China. Unicorn lotus is mainly produced in Henan, Sichuan, Shaanxi and other places, and it is also cultivated in various parts of the country. The yield in the Northeast is extremely high, and the price is relatively low. "Folk Herbal Medicine" and "Dictionary of Chinese Medicine" record that the bulbs of unicorn lotus are used for medicinal purposes, expelling cold and dampness, dispelling wind and phlegm, suppressing convulsions, treating stroke and phlegm, crooked mouth and eyes, tetanus, treating bruises, and lymphatic tuberculosis . So far, there are still few studies on this plant, which are limited to the use o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06A61P35/00
Inventor 宋艳玲鲍微泽贾金龙孙欢刘晓娟
Owner SHENYANG HONGQI PHARMA
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