Method for efficiently extracting maitake mycelia polysaccharides through submerged fermentation production

The technology of mycelium and extraction method of Grifola frondosa is applied in the field of microorganisms, which can solve the problems of increased pollution control cost, high product cost, long operation cycle, etc., and achieves reduction of pollution control cost, reduction of impurity protein content, and labor reduction. the effect of strength

Inactive Publication Date: 2014-10-01
江苏阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this process is that a large amount of water needs to be added for a long time to extract, and in order to increase the yield, it needs to be extracted repeatedly 2-3 times. %, product cost is more expensive
And the separated supernatant is directly discarded as waste liquid, which increases the cost of pollution control

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for efficiently extracting the deep layer liquid fermentation of the present invention to produce Grifola frondosa mycelia polysaccharide is carried out according to the following steps:

[0030] 1. Preparation of Grifola frondosa fermentation broth: Grifola frondosa strains were purchased from the Institute of Microbiology, Chinese Academy of Sciences (the strain number is CGMCC NO.4179). The activated Grifola frondosa shake flask strain is inoculated in the seed culture medium (10L) according to the inoculum size 10%, wherein the seed culture medium consists of glucose 1.8%, peptone 0.5%, soybean meal 1%, potassium dihydrogen phosphate 0.3% , 0.15% magnesium sulfate heptahydrate, 0.02% soybean oil, adjust the pH value to 5.5, the ventilation ratio is 1:0.3vvm, the tank pressure is 0.03Mpa, and vibrating at 26-28°C for 7 days to obtain the Grifola frondosa seed liquid; the seed liquid is pressed 15% of the inoculum was inserted into the fermentation medium (...

Embodiment 2

[0038] The method for efficiently extracting the deep layer liquid fermentation of the present invention to produce Grifola frondosa mycelia polysaccharide is carried out according to the following steps:

[0039] 1. Preparation of Grifola frondosa fermentation broth: Grifola frondosa strains were purchased from the Institute of Microbiology, Chinese Academy of Sciences (the strain number is CGMCC NO.4179). The activated Grifola frondosa shake flask bacterial strain is inoculated in the seed culture medium (20L) by inoculum size 14% (v / v), wherein the seed culture medium is composed of glucose 2.0%, peptone 0.3%, soybean meal 1%, phosphoric acid Potassium dihydrogen 0.3%, magnesium sulfate heptahydrate 0.15%, soybean oil 0.02%, adjust the pH value to 5.5, the ventilation ratio is 1:0.3vvm, the tank pressure is 0.03Mpa, shake culture at 26-28°C for 7 days to obtain Grifola frondosa seed liquid ; The seed liquid is inserted into the fermentation medium (100L) by inoculum size 18...

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Abstract

The invention discloses a method for efficiently extracting maitake mycelia polysaccharides through submerged fermentation production. The method is that mycelia separated from a maitake fermentation liquor is processed and separated through ultrasonic waves and composite enzymatic hydrolysis to obtain maitake polysaccharides. According to the invention, the conventional 'water extraction and alcohol precipitation' extraction method of polysaccharides is changed, the extraction yield and the product quality of maitake polysaccharides are improved, the consumption of purified water and energy consumption are reduced during production, and the economic benefits are increased.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a method for efficiently extracting deep-layer liquid-state fermentation to produce Grifola frondosa mycelium polysaccharide. Background technique [0002] Grifola frondosa (Dicks.Fr.) S.F.Gray belongs to Basidiomycotina, Phytomycetes, Phyllomycetes, Polyporaceae, Tree Flowers, also known as Polypore Bayeux, Thousand Buddhas, Chestnut mushrooms, cloud mushrooms, lotus mushrooms, etc. are called maitake mushrooms in Japan and forest chickens in the United States. Its meat is crisp and tender, and tastes like shredded chicken. It can be cooked into a variety of delicacies, and contains a variety of physiologically active substances. It is a kind of edible and medicinal mushroom with great development and research value. [0003] Grifola frondosa polysaccharide is the most important type of active ingredients among many biologically active substances of Grifola frondosa. It is a k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 徐玲徐国华王文风王英燕杨亚威秦建丽张芙蓉
Owner 江苏阜丰生物科技有限公司
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