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Single needle alga, as well as culture method and recovery method and application thereof

A culture method and harvesting method technology, applied in the field of microorganisms, can solve problems such as high cost, unfavorable large-scale promotion, and inability to meet industrial needs, and achieve the effect of reducing pollution

Inactive Publication Date: 2014-10-01
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still the following problems in the production of biodiesel: at present, the supply of edible oil in my country is not self-sufficient, and there is not enough surplus edible oil to develop biodiesel; the cultivation of non-edible oil crops also competes with the cultivation of grain, oil, cotton and other crops competition for water and fertilizer; the cost of producing biodiesel from animal fats is too high, which is not conducive to large-scale promotion; the production of biodiesel from waste cooking oil is limited and cannot meet the needs of industry
At present, there are few domestic and foreign reports on the strains of Monospina with bioenergy applications, and there may be excellent new strains that have not been developed in Monospina. of great significance

Method used

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  • Single needle alga, as well as culture method and recovery method and application thereof
  • Single needle alga, as well as culture method and recovery method and application thereof
  • Single needle alga, as well as culture method and recovery method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1 Screening of Monostipella C29 bacterial strain

[0083] Take 0.5mL water sample collected from a pool in Danzhou, Hainan in September 2010, spread it on BG-11 solid medium under sterile conditions, and cultivate it for 20 days. In daylight, the light intensity is 10000 lux, select a single colony and inoculate it into BG-11 liquid medium, place it on a shaker with a rotation speed of 120r / min for cultivation, and culture at 28°C for 5 days at a light intensity of 10000 lux. After testing, The pure algal strain was obtained, which was identified as the genus Monospina and named Monospina C29.

[0084] The above-mentioned Monostipella C29 was cultured in a sterilized Provasoli based minimal media in closed aseptic culture with a shaker speed of 120r / min at a culture temperature of 25-28°C for 5 days. Calculate average growth rate, maximum growth rate, biomass, oil yield.

[0085] The results of the test are as follows: the average growth rate of Monostipella C...

Embodiment 2

[0088] The optimization of embodiment 2 Monostipella C29 substratum and open culture condition

[0089] Fix the plexiglass tubes 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 in a sunny place outdoors (the light intensity is 6000-10000 lux). Tubes 1 to 10 are all colorless and transparent. 300mm, length 2000mm, and equipped with air pump and ventilation pipeline.

[0090] Divide tubes 1 to 10 into the following five groups:

[0091] Test group 1: tube 1, add 100 L of BG-11 culture medium, adopt the culture method of the present invention (see Table 2 for details).

[0092] Test group 2: tube 2, add 100 L of Provasoli based minimal media, adopt the culture method of the present invention (see Table 2 for details).

[0093] Test group 3: tube 3, add 100L of the culture medium shown in Table 1, and adopt the culture method of the prior art (see Table 2 for details).

[0094] Test group 4: Tubes 4-9 were added with 100 L of the medium shown in Table 1 respectively, and the culture method o...

Embodiment 3

[0113] The open culture of embodiment 3 Monostipella C29

[0114] Fix the plexiglass tube (colorless and transparent, with an inner diameter of 200 mm and a length of 1500 mm), an air pump and a ventilation line in an outdoor ventilated and sunny place (illumination intensity is 6000-10000 lux), and add 40 L of evenly stirred culture medium into the glass tube. The medium composition is as follows:

[0115]

[0116]

[0117] Add 5L concentration in the tube and be the seed liquid of the monospina C29 that the embodiment 1 sieves of 2g / L, pass into air culture, aeration rate is 5L / min, cultivates at 16 ℃, and the culture time is 6 days, obtains 40L of algae liquid, during the cultivation process, samples were taken every day, centrifuged and dried to measure the dry weight, and the growth curve was made according to the dry weight and culture time. Growth curve such as figure 1 shown. Depend on figure 1 It can be seen that the microalgae entered the logarithmic growth...

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Abstract

The invention relates to the technical field of microorganisms, and discloses single needle alga. The preservation number of the single needle alga is CGMCC No.8776, and the single needle alga is named single needle alga C29. The single needle alga is fast in growth speed and high in grease yield, and is an excellent bacterial strain of producing biodiesel. The invention also provides an open culture method of the single needle alga. By adopting the method, a specific culture medium is adopted to cultivate, and growth of the single needle alga C29 and accumulation of grease are facilitated. The invention also provides a recovery method of the single needle alga C29. The recovery method has nontoxicity, high flocculation efficiency and low recovery cost. The invention also provides an application of the single needle alga C29 in production of grease, especially production of biodiesel.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a single needle algae, its cultivation method, its harvesting method and its application. Background technique [0002] At present, 90% of the world's energy consumption is oil, natural gas and coal. The consumption of these fossil energy produces a large amount of gases such as carbon dioxide, sulfur dioxide, sulfur nitride and nitrogen oxides, which seriously damages the earth's environment. In addition, fossil energy is non-renewable, and long-term excessive exploitation has led to the depletion of fossil energy, which seriously restricts the development of industrial society. [0003] Therefore, countries all over the world are looking for new renewable energy sources, among which biodiesel is a clean renewable energy source and a high-quality substitute for fossil energy sources. The oil and fat raw materials of biodiesel come from vegetable oil (edible oil and non-e...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/10
Inventor 刘平怀杨勋张玲罗宁张森
Owner HAINAN UNIVERSITY
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