Chloroplast pigment paper chromatography separation method

Abstract

The invention relates to a pigment paper chromatography separation method, in particular to a chloroplast pigment paper chromatography separation method. The method includes the steps that first, pigment extract is prepared and stored in a place kept out of sun for use; then, cylindrical filter paper is made; finally, sample application is performed, after sample application pigment is dried through an electric hair drier, a small hole is made in the center of the sample application pigment, one end of the cylindrical filter paper is inserted in the small hole, the other end, namely a base end, of the cylindrical filter paper is placed in a culture dish with layer unfolding agent, the round filter paper subjected to the sample application is placed on the culture dish, and chromatography starts. After four colored ribbons (beta-carotene, lutein, chlorophyll a and chlorophyll b) are all separated, the chromatography is completed. According to the method, while the chromatography separation effect, for instance, the color of the colored ribbons is pure and the edges of the colored ribbons are clear, is improved, investment of a chromatography container is reduced and saved by 60 percent, tedious processing of the chromatography filter paper is reduced, and toxic pollution generated when acetone serves as the extraction agent, and the carbon tetrachloride serves as the layer unfolding agent is reduced. The separation method is good in separation effect, time and labor are saved, and environmental pollution and toxicity are reduced.

Description

technical field [0001] The invention relates to a chromatography separation method of pigment paper, in particular to a chromatography separation method of chloroplast pigment paper. Background technique [0002] The existing chloroplast pigment paper chromatography separation method is commonly used to separate chloroplast pigments by paper chromatography using a developing cylinder or a test tube as a chromatographic container, a filter paper strip as a stationary phase, carbon tetrachloride as a developing agent, and acetone as an extracting solution. The disadvantage of this method is that the layering cylinder or test tube has a certain height, and the filter paper strip is difficult to insert, and it is easy to touch the inner wall of the container to affect the chromatographic effect; in addition, the long filter paper strip is time-consuming and cumbersome to process, and it is difficult to use highly toxic organic solvents for four weeks. When carbon chloride is use...

AI Technical Summary

Problems solved by technology

[0002] The existing chloroplast pigment paper chromatography separation method is commonly used to separate chloroplast pigments by paper chromatography using a developing cylinder or a test tube as a chromatographic container, a filter paper strip as a stationary phase, carbon tetrachloride as a developing agent, and acetone as an extracting solution. The disadvantage of this method is that the layering cylinder or test tube has a certain height, and the filter paper strip is difficult to insert, and it is easy to touch the inner wall of the container to affect the chromatographic effect; in addition, the long filter paper strip is time-consuming and cumbersome to process, and it is difficult to use highly toxic organic solvents for four weeks. Chloride carbon as spreading agent, not only the boundary of the separation color band is not clear, but also pollutes the environment, at the same time, single acetone as the extracting agent is also harmful to the human body