Chloroplast pigment paper chromatography separation method

A separation method and paper chromatography technology, which is applied in the field of chloroplast pigment paper chromatography separation, can solve the problems of unclear boundaries of separation color bands, time-consuming filter paper strip chromatography, difficulty in inserting filter paper strips, etc., and achieve fast diffusion speed , pure color, neat edge effect

Inactive Publication Date: 2014-10-01
SHENYANG UNIV
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Problems solved by technology

[0002] The existing chloroplast pigment paper chromatography separation method is commonly used to separate chloroplast pigments by paper chromatography using a developing cylinder or a test tube as a chromatographic container, a filter paper strip as a stationary phase, carbon tetrachloride as a developing agent, and acetone as an extracting solution. The disadvantage of this method is that the layering cylinder or test tube has a certain height, and the filter paper strip is difficult

Method used

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Examples

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Embodiment

[0018] 1. Pigment extraction

[0019] Remove the thick veins from the washed fresh plant leaves, weigh 2g and cut them into pieces, put them in a mortar, add 5ml of extractant and a little calcium carbonate to the mortar, grind them into a homogenate, then add 15ml of extractant, then Filter with gauze, and the filtered residue is washed several times with the extractant until the filtered extractant after washing the residue is colorless. The above-mentioned liquid filtered out after washing the plant leaf residue with the extractant for many times is the pigment extract. Reserve in the dark; the extractant is a mixture of acetone-ethanol (1:1, v / v).

[0020] 2. Fabrication of Cylindrical Filter Paper

[0021] Cut a rectangular filter paper about 5cm long and 3cm wide, and then cut the long side of the rectangular filter paper into many curtain-like thin strips, each thin strip is about 1cm long and 2mm wide, and roll the rectangular filter paper into a small cylinder , the...

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PUM

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Abstract

The invention relates to a pigment paper chromatography separation method, in particular to a chloroplast pigment paper chromatography separation method. The method includes the steps that first, pigment extract is prepared and stored in a place kept out of sun for use; then, cylindrical filter paper is made; finally, sample application is performed, after sample application pigment is dried through an electric hair drier, a small hole is made in the center of the sample application pigment, one end of the cylindrical filter paper is inserted in the small hole, the other end, namely a base end, of the cylindrical filter paper is placed in a culture dish with layer unfolding agent, the round filter paper subjected to the sample application is placed on the culture dish, and chromatography starts. After four colored ribbons (beta-carotene, lutein, chlorophyll a and chlorophyll b) are all separated, the chromatography is completed. According to the method, while the chromatography separation effect, for instance, the color of the colored ribbons is pure and the edges of the colored ribbons are clear, is improved, investment of a chromatography container is reduced and saved by 60 percent, tedious processing of the chromatography filter paper is reduced, and toxic pollution generated when acetone serves as the extraction agent, and the carbon tetrachloride serves as the layer unfolding agent is reduced. The separation method is good in separation effect, time and labor are saved, and environmental pollution and toxicity are reduced.

Description

technical field [0001] The invention relates to a chromatography separation method of pigment paper, in particular to a chromatography separation method of chloroplast pigment paper. Background technique [0002] The existing chloroplast pigment paper chromatography separation method is commonly used to separate chloroplast pigments by paper chromatography using a developing cylinder or a test tube as a chromatographic container, a filter paper strip as a stationary phase, carbon tetrachloride as a developing agent, and acetone as an extracting solution. The disadvantage of this method is that the layering cylinder or test tube has a certain height, and the filter paper strip is difficult to insert, and it is easy to touch the inner wall of the container to affect the chromatographic effect; in addition, the long filter paper strip is time-consuming and cumbersome to process, and it is difficult to use highly toxic organic solvents for four weeks. When carbon chloride is use...

Claims

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Application Information

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IPC IPC(8): G09B23/38
Inventor 张秀君李君宇
Owner SHENYANG UNIV
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