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A method for fractionally extracting nutrients from spirulina

A technology for nutrient substance and graded extraction, applied in the field of bioengineering, can solve the problems of yield and purity of energy-consuming polysaccharides, waste of resources, inactivation of phycobiliproteins, etc., to achieve economic and environmental benefits, reduce high energy consumption and high energy consumption. Cost, improve the effect of protein denaturation and inactivation

Active Publication Date: 2016-08-31
藻能珍稀生物科技研发(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hot water extraction is often used in general tests, but it requires high temperature (above 85°C) and long time (above 8h) extraction, so this method has the disadvantages of time-consuming, energy-consuming, and the yield and purity of polysaccharides are not very high
In addition, the traditional extraction process mostly uses dry powder of spirulina as raw material, which consumes a lot of energy during the drying process and will destroy nutrients
[0004] In the current extraction process of spirulina utilization, it is often only considered to obtain one active ingredient or nutrient substance through one extraction process, such as using a higher temperature extraction in order to simply obtain a higher yield of water-soluble polysaccharides, but this inactivation of phycobiliprotein
This causes a great waste of resources, so it is very necessary to provide a method for obtaining multiple nutrients from Spirulina by fractional extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Dehydration: obtain fresh spirulina algae mud from the spirulina culture tank, accurately weigh 2g of spirulina algae mud in a 50mL centrifuge tube with an electronic balance, and add the ratio of material to liquid (algae mud: ethanol, g / g) 1:4 absolute ethanol (three parallel samples), shake well and wrap with tin foil. Place the centrifuge tube in a constant temperature shaker at 40°C and 150r / min for 15 minutes of shaking and extraction. After the extraction, centrifuge for 8 minutes in a desktop centrifuge at a speed of 5000r / min. The obtained supernatant is extracted from chlorophyll and carotenoids. solution and store it in a -20°C freezer. The algae residue was stored overnight in a refrigerator at -20°C, and then put into a freeze dryer to freeze-dry and set aside.

[0028] Determination of the extraction rate of chlorophyll and carotenoids in step (1): the algae residue after the freeze-drying in step (1) is extracted with 90% acetone solution and the pig...

Embodiment 2

[0039] 1) Dehydration-extraction of alcohol solubles: obtain fresh spirulina algae mud from the spirulina culture tank, accurately weigh 2g of spirulina algae mud in a 50mL centrifuge tube with an electronic balance, add the ratio of material to liquid (algae mud: ethanol, g / g) is 1:6 volume fraction of 80% ethanol solution (three parallel samples), shake well and wrap with tin foil. Put the centrifuge tube in a constant temperature shaker at 30°C and 200r / min for 30min and shake it for 30min. After the extraction, centrifuge for 8min in a desktop centrifuge at a speed of 8000r / min. The obtained supernatant is extracted from chlorophyll and carotenoids. solution and store it in a -20°C freezer. The algae residue was stored overnight in a refrigerator at -20°C, and then put into a freeze dryer to freeze-dry and set aside.

[0040] The algal residue after the freeze-drying of step (1) is extracted pigment with 90% acetone solution and utilizes ultraviolet-visible spectrophotome...

Embodiment 3

[0044] (1) Dehydration-extracting alcohol-soluble matter: obtain fresh spirulina algae mud from the spirulina culture tank, accurately weigh 2g of spirulina algae mud in a 50mL centrifuge tube with an electronic balance, add the ratio of material to liquid (algae mud: ethanol , g / g) with a volume fraction of 1:8 of 90% ethanol solution (three parallel samples), shake well and wrap with tin foil. Put the centrifuge tube into a constant temperature shaker at 25°C and 250r / min to vibrate for 60min. After the extraction, centrifuge for 8min in a desktop centrifuge at a speed of 14000r / min. The obtained supernatant is chlorophyll and carotenoid extract, and it is stored in a refrigerator at -20°C. The algae residue was stored overnight in a refrigerator at -20°C and then placed in a freeze dryer to freeze-dry. The algal residue after freeze-drying is extracted pigment with 90% acetone solution and utilizes ultraviolet-visible spectrophotometer to measure its absorbance at 480nm, 5...

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Abstract

The invention provides a method for extracting nutrients from spirulina in a grading manner. Mashed fresh spirulina is used as raw material and dehydrated by using high-concentration ethanol, chlorophyll and carotenoid are simultaneously extracted from the mashed spirulina, and phycobiliprotein and water-soluble polysaccharide are extracted from the spirulina residue (which is subjected to ethanol extraction) by using a phosphate buffer solution. The method has the characteristics of low energy consumption and low cost, multiple nutrients in the spirulina can be obtained by grading extraction, the extraction condition is mild, and the extracted nutrients are unlikely to be destroyed.

Description

【Technical field】 [0001] The invention belongs to the field of bioengineering, and relates to a method for extracting nutrients from spirulina by grade. 【Background technique】 [0002] Spirulina (Spirulina), also known as Arthrospira, is a kind of cyanobacteria or cyanobacteria, which provides nutrition for itself by photosynthesis, and mainly lives in salt water and brackish water. It is a nutritionally comprehensive and balanced high-protein plant food with a protein content as high as 55%-70% (of which phycobiliprotein accounts for 14%-20% of the dry weight), and water-soluble polysaccharides account for 7% of the dry weight of spirulina ~8%, and rich in pigments (such as 1% chlorophyll and 0.5% carotenoids). In addition, spirulina does not contain cholesterol, and the fat content only accounts for 5% of the dry weight. It is rich in unsaturated fatty acids such as γ-linolenic acid, which is very suitable for obese people. Because of its rich and comprehensive nutrition...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/795C07C403/24C07D487/22C08B37/00
CPCC07C403/24C07C2601/16C07D487/22C07K14/795C08B37/0003
Inventor 魏东马超
Owner 藻能珍稀生物科技研发(广州)有限公司
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