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Solid fermentation ginseng flower and preparation method thereof

A solid fermentation, ginseng flower technology, applied in biochemical equipment and methods, fermentation, microbial-based methods, etc., can solve rare and other problems

Active Publication Date: 2014-10-08
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, the reports on the microbial transformation of ginsenosides (including liquid fermentation and solid fermentation) are mostly concentrated on the research on ginseng roots, while the reports on microbial transformation of other parts such as ginseng leaves, ginseng flower buds and ginseng fruits are rare, especially It is the study of microbial solid fermentation of ginseng flower, which is rarely reported at home and abroad

Method used

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  • Solid fermentation ginseng flower and preparation method thereof
  • Solid fermentation ginseng flower and preparation method thereof
  • Solid fermentation ginseng flower and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 preparation culture medium

[0033] C-Y medium: peptone 10g, yeast powder 5g, glucose 20g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganese sulfate 0.2g, ferrous sulfate 0.03g, potassium dihydrogen phosphate 6g, polyoxyethylene dehydration Sorbitan monooleate 1mL, dissolved in 1L water, adjust pH 5.85.

[0034] C-Y-1 medium: peptone 10g, yeast powder 5g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganese sulfate 0.2g, ferrous sulfate 0.03g, potassium dihydrogen phosphate 6g, polyoxyethylene dehydrated sorrel Alcohol monooleate 1mL, dissolved in 1L water, adjust pH 5.85.

[0035] C-Y-2 medium: glucose 20g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganese sulfate 0.2g, ferrous sulfate 0.03g, potassium dihydrogen phosphate 6g, polyoxyethylene sorbitan monooleic acid Dissolve 1 mL of ester in 1 L of water and adjust the pH to 6.05.

[0036] The above-mentioned medium...

Embodiment 2

[0038] Example 2 Identification of plantarum lactobacillus producing β-glucosidase

[0039] Perform aseptic operation in an ultra-clean workbench, pour the prepared aescin-R2A solid medium onto the plate, and after the medium is cooled and solidified, pick Lactobacillus plantarum (Lactobacillus plantarum) with an inoculation loop and spread it on the plate One side and the other side as blank treatment, move the plate from the ultra-clean workbench into a biochemical incubator, set the culture temperature at 37°C, and culture it statically for 48 hours, take out the plate and observe the color of the medium-coated side and the blank treatment change, resulting in the coated side turning black (see figure 1 ).

Embodiment 3

[0040] The selection of embodiment 3 seed culture medium

[0041] Pour the prepared medium onto the plate, and after the medium is cooled and solidified, pick Lactobacillus plantarum with an inoculation loop, inoculate the strains in the medium by 4-section line inoculation, move them into the biochemical incubator, and set The culture temperature was 37°C, and after 48 hours of static culture, the growth characteristics of the strain were observed. After Lactobacillus plantarum was inoculated into the C-Y medium, the growth of the colony was significantly better than that of the SL medium, such as figure 2 shown.

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Abstract

The invention discloses a solid fermentation ginseng flower and a preparation method thereof. The preparation method comprises the following steps: uniformly placing sterilized ginseng flowers on the surface of an SL solid culture medium without a carbon source, spraying a lactobacillus plantarum seed solution, and carrying out sealed fermentation at 35 DEG C. According to the preparation method, an SL solid culture medium C-Y-1 without the carbon source is adopted; when the fermentation is carried out for 1 day, the content of ginseng total saponins is 160.230 mg / g, is increased by 76.215 mg / g compared with that of a negative tested product and increased by 1.91 times, the content of Rg3 is 0.076 mg / g, and the Rg3 is not detected in the negative tested product; when the fermentation is carried out for 3 days, the content of Rh2 is increased by 0.612 mg compared with that of the negative tested product and increased by 2.34 times; when the fermentation is carried out for 1 day, the content of F1 is increased by 0.156 mg compared with that of the negative tested product and increased by 1.2 times; when the fermentation is carried out for 7 days, the content of F2 is increased by 0.106 mg compared with that of the negative tested product and increased by 3.04 times.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to a solid fermented ginseng flower and a preparation method thereof. Background technique [0002] Solid-state fermentation can usually be defined as the growth process and biological reaction process of microorganisms that contain no or almost no free water culture substrate. [0003] The microbial strains used for solid fermentation grow, reproduce and produce metabolites on a medium with little free water, and the process belongs to biological reactions. According to the purpose of fermentation, it can be classified into two basic types: 1. The purpose is to grow and cultivate microorganisms or spores; 2. The purpose is to produce enzymatic reactions and metabolites. However, some fermentation processes include the growth and cultivation of microorganisms, as well as the production of enzymatic hydrolysis and metabolites, such as the cultivation of distill...

Claims

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Application Information

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IPC IPC(8): C12P33/00A61K31/704C12R1/25
Inventor 王义张美萍蒋世翠孙春玉王康宇陈旸孙亮
Owner JILIN AGRICULTURAL UNIV
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