A method for extracting oligosaccharides from rice germ embryos for hplc detection
An extraction method and oligosaccharide technology, which is applied in the field of analytical chemistry, can solve the problems of large differences in components between soybean seeds and rice seeds, long time, etc., and achieve the effect of reliable extraction method, less samples, and guaranteed accuracy
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Embodiment 1
[0032] (1) Take 10 fresh seed embryos of rice harvested 22 days after pollination, weigh them, grind them into powder at room temperature, add 1ml of 80% ethanol solution (solid-to-liquid ratio is 1:150), grind them evenly, and then place them in 80 ℃ shake and extract in a constant temperature shaking water bath for 30 minutes. After shaking extraction, the mixture was brought to room temperature, and then centrifuged at 16000r / min for 15min to obtain the first extraction supernatant and solid residue.
[0033] (2) Add 0.5ml of 80% ethanol solution (solid-to-liquid ratio: 1:100) to the solid residue after the first extraction, mix well and place it in an ultrasonic oscillator for extraction for 30min, with an ultrasonic power of 80W, and the ultrasonic extraction ends Afterwards, centrifuge at 16000r / min for 15min to obtain the second extraction supernatant.
[0034] (3) Combine the two extraction supernatants, and dry them with nitrogen gas at room temperature (about two ho...
Embodiment 2
[0043] During ultrasonic extraction, the ultrasonic temperature is 20° C., and the ultrasonic time is set at three levels of 20 min, 30 min, and 50 min. The extraction process of the remaining oligosaccharides is the same as in Example 1.
[0044] Table 2 The effect of ultrasonic time on the extraction of rice embryo oligosaccharides
[0045]
[0046] *CK refers to the result without ultrasonic treatment in Example 1, and different lowercase letters in the table indicate the significant difference between treatments of the same oligosaccharide (α=0.05, LSD).
[0047] The results are shown in Table 2. The extraction effect of oligosaccharides is better when the ultrasonic time is 30 min. The content of the extracted oligosaccharides has no significant difference with the result of Example 1, and the content of the extracted oligosaccharides does not increase significantly when the ultrasonic time is continued to increase. .
Embodiment 3
[0049] During ultrasonic extraction, the ultrasonic temperature was 20°C, the ultrasonic time was 30min, and the nitrogen drying temperature was set at three levels of 25°C, 35°C and 45°C, and the rest of the process was the same as in Example 1.
[0050] Table 3 The influence of nitrogen blowing temperature on the concentration rate of the extract
[0051]
[0052] *CK refers to the time required for drying with nitrogen at room temperature, and the different lowercase letters in the table indicate the significant difference in the concentration time of the extract between treatments (α=0.05, LSD).
[0053] Table 4 The effect of nitrogen blowing temperature on the extraction of oligosaccharides from rice germ embryo
[0054]
[0055] *CK refers to nitrogen drying treatment at room temperature, and different lowercase letters in the table indicate significant differences among treatments of the same oligosaccharide (α=0.05, LSD).
[0056] As shown in Table 3 and Table 4...
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