Application of gastrodia tuber polysaccharide in preparation of functional food with effect of helping inhibit colon cancer

A technology of Gastrodia elata polysaccharide and functional food, applied in the field of polysaccharides, can solve the problems of preparing polysaccharide components and the like, achieve the effects of high purity and activity, auxiliary inhibition of colon cancer tumors, and good colon cancer tumors

Inactive Publication Date: 2014-10-29
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no polysaccharide component prepared from Gastrodia elata as a raw material,

Method used

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  • Application of gastrodia tuber polysaccharide in preparation of functional food with effect of helping inhibit colon cancer
  • Application of gastrodia tuber polysaccharide in preparation of functional food with effect of helping inhibit colon cancer
  • Application of gastrodia tuber polysaccharide in preparation of functional food with effect of helping inhibit colon cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The Gastrodia elata polysaccharide in this example is prepared by the following method: take the Gastrodia elata raw material and extract it with boiling water. When extracting for the first time, the amount of water used is 20 times the total mass of the raw material, and it is boiled for 5 h to filter; The amount of water used is 20 times the total mass of raw materials, boiled for 3 h and filtered; for the third extraction, the amount of water used is 10 times the total mass of raw materials, boiled for 2 h and filtered, combined filtrates, vacuum concentrated to about 1 / of the original volume 10, the same below. The concentrated solution was treated with trichloroacetic acid to remove protein, and the same volume (the same below) of 30% trichloroacetic acid (mass percentage) was added. After the treatment time was 4 hours, it was neutralized with 10% NaOH (mass percentage), the same below, After neutralization, place the reaction solution in a dialysis bag and tie ...

Embodiment 2

[0032] The Gastrodia elata polysaccharide in this example is prepared by the following method: take the gastrodia elata raw material, extract it with boiling water, use 15 times the total mass of the gastrodia elata raw material for the first extraction, boil for 5 h and filter; , the amount of water used was 15 times the total mass of Gastrodia elata raw materials, boiled for 3 h and filtered; for the third extraction, the amount of water used was 10 times the total mass of Gastrodia elata raw materials, boiled for 3 h and filtered, combined filtrates, and concentrated in vacuo. The concentrated solution was treated with trichloroacetic acid to remove protein, and the same volume of 25% trichloroacetic acid (mass percentage) was added. After the treatment time was 3 h, it was neutralized with 10% NaOH (mass percentage). After neutralization, the reaction solution was placed in Dialyze in a dialysis bag, then concentrate the liquid in the dialysis bag, centrifuge, take the supe...

Embodiment 3

[0034] The Gastrodia elata polysaccharide in this example is prepared by the following method: take the Gastrodia elata raw material and extract it with boiling water. When extracting for the first time, the amount of water used is 10 times the total mass of the gastrodia elata raw material, boil for 3 h and filter; , the amount of water is 20 times the total mass of Gastrodia elata raw materials, boiled for 3 h and filtered for 3 hours; for the third extraction, the amount of water used is 10 times the total mass of Gastrodia elata raw materials, boiled for 5 h and filtered, combined filtrates, concentrated in vacuo, and concentrated solution Perform trichloroacetic acid protein removal treatment, add the same volume of 15% trichloroacetic acid (mass percentage), after a treatment time of 5 hours, neutralize with 10% NaOH (mass percentage), and place the reaction solution in a dialysis bag after neutralization Dialyze in the middle bag, then concentrate the liquid in the dialy...

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Abstract

The invention discloses application of gastrodia tuber polysaccharide in preparation of functional food with the effect of helping inhibit colon cancer. The gastrodia tuber polysaccharide is preferably prepared by the following method: extracting gastrodia tuber taken as a raw material with boiling water, removing protein, dialyzing and carrying out ethanol treatment. The gastrodia tuber polysaccharide has the function of adjusting the tumor microenvironment. The gastrodia tuber polysaccharide can adjust the tumor microenvironment through multiple ways such as inhabitation of tumor angiogenesis, activation of immunity and inhabitation of fibroblast growth of the colon cancer, so that the gastrodia tuber polysaccharide has a good effect of helping inhibit colon tumors.

Description

technical field [0001] The invention belongs to the technical field of polysaccharides, and in particular relates to the application of Gastrodia elata polysaccharides in the preparation of functional foods with the effect of assisting in inhibiting colon cancer. Background technique [0002] The study of immune negative regulation provides a new strategy for tumor therapy. In the tumor microenvironment, there are a large number of negative regulatory cells, which interact and influence each other, accompanied by the co-evolution of tumor cells and the microenvironment. Due to the immunogenic changes and abnormal growth of tumor cells, fibroblasts are stimulated to release inflammatory factors, antigen-presenting cells transmit tumor antigen signals, and jointly recruit immune cells to infiltrate into the tumor, and the abnormal proliferation of tumor cells causes damage to adjacent cells. Compression, leading to ischemic necrosis and other tissue damage, produces a large n...

Claims

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Application Information

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IPC IPC(8): A23L1/09A61K31/715C08B37/00A61P35/00
CPCA23L29/30A23V2002/00C08B37/0003A23V2200/308A23V2250/51A23V2250/21A23V2300/14
Inventor 胡明华王培培马方励梁明
Owner INFINITUS (CHINA) CO LTD
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