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Mouse uterus perfusion apparatus and method for establishing mouse endometritis model

An endometritis and perfusion device technology, applied in the medical field, can solve the problems of establishing a mouse endometritis model, difficulty in perfusion, perfusion fluid perfusion, etc., to reduce mechanical damage, shorten operation time, and reduce outflow Effect

Inactive Publication Date: 2014-11-05
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The perfusion method is not described in this paper, and the perfusion volume is too high to be suitable for mice
However, due to the small size of the mice, the perfusion is difficult, and it is difficult to successfully perfuse the perfusate into the mouse uterus with the usual technical means, so it is difficult to establish a mouse endometritis model by perfusion.

Method used

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  • Mouse uterus perfusion apparatus and method for establishing mouse endometritis model
  • Mouse uterus perfusion apparatus and method for establishing mouse endometritis model
  • Mouse uterus perfusion apparatus and method for establishing mouse endometritis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Determination of the volume of perfusate in the mouse uterus

[0042] In order to determine the optimum volume of perfusate in the mouse uterus, this experiment perfused 10, 20, and 40 μl of PBS in the mouse uterus respectively, observed the performance of the mice after perfusion, and determined the volume of the perfusate in the mouse uterus. It was found that when the volume of perfusate in the uterus of each mouse was 40 μl, the perfusion was difficult and the vulva fluid flowed out after perfusion; if the perfusion was 10 μl, although the perfusion fluid could completely enter the uterus, the perfusion fluid could not be evenly distributed. Therefore, the volume of mouse uterine perfusate was finally determined to be 20 μl / mouse.

Embodiment 2

[0043] Embodiment 2: Comparison of anesthesia methods

[0044] In order to compare the effects of different general anesthesia methods on the establishment of this model, a comparative test of anesthesia was carried out with inhalation anesthetics such as ether and Sumianxin injection. Twelve mice were divided into ether anesthesia group and Sumianxin anesthesia group. Ether anesthetized group: place the mouse upside down in a beaker, and put a cotton ball soaked in ether into the beaker. Sumianxin anesthesia group: Sumianxin injection was injected into the rear thigh muscle of mice at a dose of 0.67ml / kg. After anesthesia, 20 μL lipopolysaccharide with a concentration of 2.5 mg / mL was perfused in the uterus, and the change of NO content in the uterus of mice was detected 24 hours after perfusion.

[0045] It takes 5-10 minutes for the mice to go from the injection of Sumianxin to general anesthesia, and the maintenance period can be maintained for 1-2 hours. However, the m...

Embodiment 3

[0046] Example 3: Screening of LPS concentration and time in the mouse endometrium inflammation model

[0047] The key to the establishment of the mouse endometrial inflammation model is the concentration of the modeling drug and the time when the obvious inflammatory response appears. The appropriate concentration and time range can only be determined by designing concentration gradients and time gradients for screening.

[0048] 1.1 Test animals

[0049] 120 8-12-week-old clean-grade Kunming female mice (purchased from Qingdao Drug Control Institute), weighing 30-35 g. 12h of light alternates with 12h of darkness, the indoor temperature is 20-25°C, and feed and drinking water are given normally. After one week of adaptive feeding, it will be ready for use.

[0050] 1.2 Main reagents and kits

[0051] LPS (Sigma, O127: B8); Sumianxin Injection (Dunhua Shengda Animal Pharmaceutical Co., Ltd., Jilin Province, approval number: Veterinary Medicine (2010) 070031582); Myelopero...

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Abstract

The invention provides a method for establishing a mouse endometritis model. The method includes the steps that female mice of 8-12 weeks old are used as test animals, after the mice are subjected to general anesthesia, a mouse uterus perfusion apparatus is used for injecting 20 microliters of 2.5-5.0 mg / ml lipopolysaccharide (LPS) into each mouse uterus, and after 12-24 hours, it is judged that the model is successfully established by observing the infiltration condition of inflammatory cells in mouse uterus tissue slices and the content change of mouse uterus nitric oxide (NO) and myeloperoxidase (MPO). The invention further discloses the mouse uterus perfusion apparatus used for injecting drugs. The perfusion apparatus is simple in structure and reasonable in design, and medicine can be injected into mouse uteruses conveniently and rapidly. Compared with an existing method for establishing the endometritis model, the damage of operation techniques to experimental animal organisms is reduced, the model better approaches to a natural paroxysm, the interference with test results is small, the success rate is high, operation is simple, and an appropriate animal disease model is provided for diagnosis, treatment, prevention and control of endometritis.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to a method for establishing an animal disease model, in particular to a method for establishing a mouse endometritis model and a perfusion tool used in the establishment of the model. Background technique [0002] Endometritis is one of the common obstetric diseases in dairy cows. In order to obtain better therapeutic effects, newly developed drugs generally choose to establish endometritis models for therapeutic research, which not only avoids economic losses caused by damage to sick animals, but also allows for objective evaluation of therapeutic effects. The main method of establishing the model is to inoculate the uterus with the bacterial solution, and the methods for inoculating the uterus with the bacterial solution usually include the following: (1) inject after relaxing the cervix with estrogen, but estrogen will inhibit the growth of Escherichia coli in the uterus. The ad...

Claims

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Application Information

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IPC IPC(8): A61D7/00A61K31/739
Inventor 曹荣峰付开强王继芳吕晓珮李伟师
Owner QINGDAO AGRI UNIV
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