Preparation method for impatiens balsamina with pine wood nematode killing effect
A technology for pine wood nematodes and impatiens, which is applied in the field of botanical pesticides and can solve problems such as extensive maintenance and management
Inactive Publication Date: 2014-11-12
NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
View PDF0 Cites 3 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, there are no data reports and products related to the preparation of pesticides. Taking advantage of the strong adaptability of Impatiens, drought and barren resistance, rapid growth, and extensive maintenance and management, vigorously develop related pesticide products, which can not only increase the added value of the products, but also increase the value of the products. Can control the harm of pine xylophilus and reduce economic losses
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0027] 1. Sample preparation:
[0028] Fresh impatiens stems were freeze-dried, weighed 50 g of the freeze-dried samples, crushed them, passed through a 40-mesh sieve, added 300 mL of 55% ethanol for leakage extraction at room temperature, filtered the leakage through Xinhua filter paper, and used rotary evaporation for the filtrate The ethanol was evaporated under reduced pressure at 45 °C, and the crude extract was freeze-dried with a yield of 18.62%, and stored at -20 °C for future use.
[0029] 2. Caenorhabditis elegans experiment:
[0030] Wild-type Caenorhabditis elegans C.elegans Bristol N2, nematodes at the L1 stage at the same developmental stage (synchronized for 24 h) were randomly added to a 12-well plate (each well contained 50 worms), and K-medium was used as the culture medium, and added to the large intestine Bacillus EColi.OP50 was used as food, and was exposed to different concentrations of impatiens flower stem extract and blank control for 48 hours (cultiv...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
Disclosed is a preparation method for impatiens balsamina with the pine wood nematode killing effect. The method comprises the steps that fresh impatiens balsamina and dry impatiens balsamina obtained through drying in the sun respectively comprise a whole plant and the summation of one or two or more of stems, leaves, flowers, seeds and roots and are directly juiced to be applied, and residues and extractives extracted from the fresh impatiens balsamina and the dry impatiens balsamina obtained through drying in the sun through a single organic solvent or two or more mixed organic solvents of petroleum ether, normal hexane, cyclohexane, cyclopentane, benzene, toluene, diethyl ether, chloroform, carbon tetrachloride, dichloromethane, acetic ether, acetone, butanone, isopropanol, normal propyl alcohol, normal butanol, ethyl alcohols of different concentrations, carbinol, water and the like are directly applied or made into different preparations. The preparation method is simple and easy to implement, the pine wood nematode prevention and treatment cost is reduced, and the pine wood nematode can be effectively killed in time on multiple aspects such as survival rate reduction, motor behavior reduction caused by damage to nervous systems and reproductive capacity reduction.
Description
technical field [0001] The invention relates to the technical field of botanical pesticides, in particular to a method for preparing impatiens which can kill pine wood nematodes. Background technique [0002] Impatiens ( Impatiens balsamina L) Balsaminaceae (Balsaminaceae) Balsaminaceae ( Impatiens )Annual herb (alias: bone grass, henna, little pink, impatiens, dry pearl, etc.). There are more than 900 species of plants in the genus Impatiens, which are distributed all over the world. Impatiens ( Impatiens balsamina L.) is planted as an ornamental plant at home and abroad, and it is also a common species of this genus. Its whole plant, flowers, stems, leaves, peels, seeds, and roots have a long history of medicinal use in the folk, and are widely used to treat waist Hypochondriac pain, snake bites, goosefoot, tinea pedis, onychomycosis, paronychia, rheumatoid arthritis, carbuncle boils, boils, retained placenta, persistent ulcers and amenorrhea, etc., 2010 edition of ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): A01N65/08A01P5/00
Inventor 姜洪芳张卫明壮子恒
Owner NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP