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Cotton fiber specific MYBL1 gene

A gene and gene sequence technology, applied in the field of cotton fiber quality improvement, can solve problems such as the inability to use high value-added and high-quality textile products to produce raw materials and poor cotton fiber quality

Inactive Publication Date: 2014-11-19
HUAZHONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country's cotton output ranks first in the world, but the quality of cotton fiber is poor and cannot be used as raw materials for some high-value-added high-quality textile products

Method used

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  • Cotton fiber specific MYBL1 gene
  • Cotton fiber specific MYBL1 gene
  • Cotton fiber specific MYBL1 gene

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Embodiment Construction

[0040] 1. Genomic DNA sequence and cDNA isolation and cloning of cotton GhMYBL1 gene and promoter

[0041] A full-length cDNA of a new MYB family gene was isolated from a cotton fiber cDNA library (according to Li XB et al., 2002, Molecular characterization of the cotton GhTUB1gene that is preferentially expressed in fiber.Plant Physiol 130:666-674) The sequence, as shown in SEQ.ID.No.2, is named GhMYBL1. The amino acid sequence of the encoded protein is shown in SEQ.ID.No.3. In order to obtain the genomic DNA sequence of the GhMYBL1 gene, design and synthesize primers at both ends of the open reading frame (ORF) according to the above cDNA sequence, and use cotton genomic DNA as a template, at 94 ° C for 5 min, 1 cycle; at 94 ° C for 1 min, 58 1 min at °C, 2 min at 72 °C, 32 cycles; finally, a 10 min extension at 72 °C was amplified to obtain a DNA fragment of about 1.5 kb (the sequence of the gene coding region, excluding the promoter sequence). This DNA fragment was inser...

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Abstract

The invention relates to a cotton fiber specific MYBL1 gene. The gene sequence overall length is 3789bp, which is shown in the sequence table SEQIDNo.1, wherein first 2017 bp fragments of the initiation codon (ATG) are promoter sequences; two introns are inserted into a gene coding region and are respectively inserted into the forty-fifth-bit and eighty-eighth-bit codon sites. The length of the cDNA of the gene is 1264 bp, which is shown in sequence table SEQIDNo.2; the open reading frame is 975 bp and one protein containing 324 amino acids is coded. The specific expression of GhMYBL1 is carried out in cotton fiber cell secondary wall synthesis period, and GhMYBL1 protein can form homodimer. Overexpression of GhMYBL1 in arabidopsis thaliana can cause that the cell walls of the fibrocyte between vascular bundles, duct cells and lignified fiber cells in mature stem xylem of transgenic arabidopsis are thickened. The above results show that the GhMYBL1 likely participates in regulation of cotton fiber cell secondary wall development, therefore, the gene and the fiber specific promoter thereof have an important application value to improvement of cotton fiber quality.

Description

Technical field: [0001] The invention relates to a cotton gene and its specific regulatory element (promoter), in particular to a new member gene MYBL1 in the cotton R2R3MYB family, which is further applied to the improvement of cotton fiber quality. Background technique: [0002] The cell wall is the membrane covering the protoplast at the outermost layer of the cell. As a complex dynamic structure, the plant cell wall plays an important role in the development of plants. Secondary cell wall (SCW) exists in plant cells undergoing secondary growth. The secondary cell wall is a layer of thickened structure that continues to accumulate inside the primary cell wall (PCW) after plant cells stop growing. The components are cellulose, hemicellulose and lignin. Secondary cell walls store a large amount of solar energy and carbon sources fixed by plant photosynthesis, and play an important role in human life, providing important raw materials such as edible fiber, textile fiber, wo...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 李学宝龚思颖孙翔聂晓莹
Owner HUAZHONG NORMAL UNIV
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