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Low-moisture raw material solid fermentation method for assimilating inorganic nitrogen of fiber byproducts

A solid-state fermentation and by-product technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as economic infeasibility, and achieve the effect of increasing crude protein content

Inactive Publication Date: 2014-11-26
山东泰山生力源集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is processed by aerobic solid-state fermentation, which can increase its protein content, but this is the respiration and metabolism of microorganisms to discharge CO 2 , at the cost of the loss of dry matter of the fermentation material, it is not economically feasible. Only by assimilating inorganic nitrogen to increase the protein content can the product quality and economic benefits be truly improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: The measured crude protein content of corn germ meal is 17.4%, 100% corn germ meal is added with water to a moisture content of 30%, the material is then added with corn germ meal weight ratio of 6% ammonium sulfate, mixed evenly, and the pH of the material is 3.5 , the material is directly inoculated with Aspergillus oryzae evenly without sterilization, the material temperature is controlled at 30-40°C, and the temperature is ventilated and cooled above 40°C. The solid-state fermentation of the raw material is completed for 48 hours, dried to a moisture content of 8-12%, and the crude protein is weighed and measured. content, inorganic nitrogen content, material weight recovery rate of 84.5%, crude protein content of 27.9%, inorganic nitrogen content of 0.23%, crude protein content increased by 60% compared with raw materials.

Embodiment 2

[0015] Example 2: Mix 30% corn germ meal and 70% bran, the crude protein content is 16.2%, add ammonium sulfate with a weight ratio of 3% to the material, add water to a moisture content of 40%, mix evenly, and the pH of the material is 5 .0, the material is directly uniformly inoculated with Aspergillus oryzae strains without sterilization, the temperature of the material is controlled at 30-40°C, and the temperature exceeds 40°C with ventilation and cooling, the solid-state fermentation of the raw meal is completed for 24 hours, dried until the moisture content is 8-12%, weighed and measured Crude protein content and inorganic nitrogen content, the weight recovery rate is 78.8%, the crude protein content is 24.5%, and the inorganic nitrogen content is 0.16%. The crude protein content is 51% higher than that of the original material.

Embodiment 3

[0016] Example 3: Mix 20% corn germ meal, 20% sprayed corn bran and 60% bran, the crude protein content of the mixed material is 17.6%, then add ammonium sulfate with a weight ratio of 2% of the mixed material, and add water to a moisture content of 35% , mixed evenly, the pH of the material is 4.2, the material is directly inoculated with Aspergillus niger evenly without sterilization, the temperature of the material is controlled at 30-40°C, and the temperature is ventilated and cooled above 40°C, the solid-state fermentation of the raw material is completed for 36 hours, and dried until the moisture content is 8-12 %, weigh and measure the crude protein content and inorganic nitrogen content, the weight recovery rate is 83.8%, the crude protein content is 23.8%, the inorganic nitrogen content is 0.21%, and the crude protein content is increased by 35%.

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PUM

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Abstract

The invention relates to a low-moisture raw material solid fermentation method for assimilating inorganic nitrogen of fiber byproducts. The low-moisture raw material solid fermentation method assimilating inorganic nitrogen of the fiber byproducts comprises the steps of adding the fiber byproducts into corn byproducts, mixing uniformly to form a mixed material, corn germ meal accounting for 20-100% of the mixed material; adding ammonium sulfate after the materials are mixed uniformly, a weight ratio of the mixed material to ammonium sulfate being 100:2-6; adding water until the moisture content of the material is 30-40%; inoculating mycelial fungi without sterilization, fermenting the raw material for 24-48 hours; and drying the material until moisture content is 8-12%, and thus a solid fermentation process is finished. Key points of the method lie in that inorganic nitrogen is assimilated and conversed into organic protein through the solid fermentation by using the mycelial fungi; acid fiber byproducts with a pH of 3.5-5.0 and 30-40% low-moisture environment are used to limit growth of bacteria, so that raw material solid fermentation of the mycelial fungi is realized; sterilization cost for the material can be saved; cost for drying the material can be reduced; and the content of crude protein of the raw material can be increased by 35-60%.

Description

[0001] technical field [0002] The invention relates to a low-moisture raw material solid-state fermentation method for assimilating inorganic nitrogen by fiber by-products, in particular a raw material solid-state fermentation method for increasing the protein content of food processing fiber by-products under low-moisture conditions. The sensitivity of fungi and bacteria to the water activity and pH of solid-state fermentation substrates is different. Under low moisture conditions, low-pH acidic fiber substrates are used for solid-state fermentation of raw materials. At the same time, filamentous fungi are used to assimilate inorganic nitrogen to synthesize organic proteins, which belongs to solid state The field of fermentation technology. Background technique [0003] Solid-state fermentation refers to the process of cultivating microorganisms in wet solid materials that contain no or almost no free water. It is used in many fields such as brewing food, biological feed,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12R1/69C12R1/685
Inventor 李军训
Owner 山东泰山生力源集团股份有限公司