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Nano-micelle capable of realizing integration of diagnosis and treatment, as well as preparation method and application of nano-micelle

A nano-micelle and carrier technology, which is applied in the field of nano-micelle and its preparation for the integration of diagnosis and treatment, can solve the problems of low luminous efficiency and easy quenching, achieve high luminous efficiency, reduce damage, and realize the effect of integrated diagnosis and treatment

Active Publication Date: 2014-12-03
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] One of the objectives of the present invention is to provide a nano-micelle with integrated diagnosis and treatment, which overcomes the disadvantages of low luminescence efficiency and easy quenching of traditional dyes; good enrichment

Method used

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  • Nano-micelle capable of realizing integration of diagnosis and treatment, as well as preparation method and application of nano-micelle
  • Nano-micelle capable of realizing integration of diagnosis and treatment, as well as preparation method and application of nano-micelle
  • Nano-micelle capable of realizing integration of diagnosis and treatment, as well as preparation method and application of nano-micelle

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Embodiment 1

[0032] Preparation of nano micelles of the present invention

[0033] (1) Dissolve AIE dye (1mg / mL) and doxorubicin (0.5mg / mL) in chloroform respectively to make solutions for use. Use a centrifuge tube with a volume of 5 mL to weigh 10 mg of the wrapping material and dissolve it in 2 mL of chloroform, transfer the solution of the wrapping material to an eggplant-shaped flask with a volume of 50 mL, rinse the centrifuge tube twice with 2 mL of chloroform, and Transfer the rinsing solution to an eggplant-shaped flask, then add 1mL of AIE solution and 2mL of doxorubicin solution into the eggplant-shaped flask, and finally add 3mL of chloroform to the eggplant-shaped flask and shake well.

[0034](2) The mixed solution in step (1) was subjected to vacuum rotary evaporation with reference to the method disclosed in Pharmaceutics (People's Health Publishing House, published in 2007) to obtain 12 mg of dried material.

[0035] (3) Add 2 mL of triple-distilled water to the eggplant-...

Embodiment 2

[0037] Nano micelles of the present invention can be prepared by the following method

[0038] (1) Dissolve AIE dye (1mg / mL) and doxorubicin (0.5mg / mL) in chloroform respectively to make solutions for use. Use a centrifuge tube with a volume of 5 mL to weigh 20 mg of the wrapping material and dissolve it in 3 mL of chloroform, transfer the solution of the wrapping material to an eggplant-shaped flask with a volume of 100 mL, rinse the centrifuge tube twice with 5 mL of chloroform, and Transfer the rinsing solution to an eggplant-shaped flask, then add 2mL of AIE solution and 4mL of doxorubicin solution into the eggplant-shaped flask, and finally add 6mL of chloroform to the eggplant-shaped flask and shake well.

[0039] (2) The mixed solution in step (1) was subjected to vacuum rotary evaporation with reference to the method disclosed in Pharmaceutics (People's Health Publishing House, published in 2007) to obtain 24 mg of dried material.

[0040] (3) Add 2 mL of triple-disti...

Embodiment 3

[0042] Nano micelles of the present invention can be prepared by the following method

[0043] (1) Dissolve AIE dye (1mg / mL) and doxorubicin (0.5mg / mL) in chloroform respectively to make solutions for use. Use a centrifuge tube with a volume of 5 mL to weigh 10 mg of the wrapping material and dissolve it in 2 mL of chloroform, transfer the solution of the wrapping material to an eggplant-shaped flask with a volume of 50 mL, rinse the centrifuge tube twice with 2 mL of chloroform, and Transfer the rinsing solution to an eggplant-shaped flask, then add 1mL of AIE solution and 2mL of doxorubicin solution into the eggplant-shaped flask, and finally add 3mL of chloroform to the eggplant-shaped flask and shake well.

[0044] (2) The mixed solution in step (1) was subjected to vacuum rotary evaporation with reference to the method disclosed in Pharmaceutics (People's Health Publishing House, published in 2007) to obtain 12 mg of dried material.

[0045] (3) Add 2 mL of physiological...

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Abstract

The invention discloses a nano-micelle. The nano-micelle comprises an AIE (aggregation-induced emission) dye, an active pharmaceutical ingredient and an amphipathic carrier in a mass ratio of 1 to 1 to (5-10). The invention further discloses a preparation method of the nano-micelle and application of the nano-micelle in preparing tumor diagnosing and / or treating agents. The nano-micelle is capable of realizing integration of diagnosis and treatment, is used for in-vivo non-invasive fluorescence imaging, diagnosis and disease treatment, and can be well gathered on a tumor site by sufficiently utilizing the EPR effect of a tumor, so that the active pharmaceutical ingredient carried by the nano-micelle can be enriched on the tumor site to play a role in effectively treating the tumor; furthermore, an AIE red-light dye is high in luminous efficiency, is not easy to quench, is capable of well reflecting the situation of delivery and biological distribution of the active pharmaceutical ingredient in a body, and can be used for diagnosing tumors.

Description

technical field [0001] The invention belongs to the chemical field of pharmaceutical active ingredients, and relates to a nanomicelle and its preparation method and application, in particular to a nanomicelle comprising an aggregation-induced luminescent red fluorescent dye, a pharmaceutical active ingredient and a carrier to realize the integration of diagnosis and treatment And its preparation method and application Background technique [0002] Tumor treatment and diagnosis has always been a very popular research direction, and many research groups have reported systems for non-invasive diagnosis and treatment of tumors. In vivo bioluminescence imaging is one of the most commonly used diagnostic methods, which enables researchers to directly and rapidly measure tumor growth, metastasis, and response to active ingredients of drugs in various tumor models. In vivo bioluminescent imaging is characterized by extremely high sensitivity, which can be used for the detection of ...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/18A61K9/107A61K31/704A61K31/337A61K31/12A61K47/34A61K47/24A61P35/00
Inventor 梁兴杰李鸿基安菲菲李婵张吉梅
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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