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A kind of method for in vitro culture of long tube Lycoris seeds

A technology of in vitro culture and long tube of Lycoris is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, and can solve the problems of inability of seeds to mature, low success rate of seeds, restriction of research and development of new varieties, etc. The effect of improving the success rate

Active Publication Date: 2016-08-24
邓波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, for distant hybrid varieties, due to the incompatibility of hybridization, the plant embryos in the fertilized ovary after hybridization are basically aborted or degenerated in the early developmental stage, and the seeds cannot be matured, and basically the harvest is not mature. Therefore, the success rate of obtaining mature and germinating seeds after distant hybridization is very low, which will limit the development of new varieties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The method for in vitro culture of Lycoris long tube seed described in the present embodiment, comprises the following steps:

[0018] Step 1. Selection and disinfection of the fertilized ovary: select the blooming long-tube Lycoris flower and remove the perianth and receptacle of the long-tube Lycoris flower, and only keep the complete fertilized ovary. At this time, the fertilized ovary is immature seeds; the complete fertilized ovary epidermis was washed and placed on an ultra-clean workbench for sterilization: rinsed with alcohol with a mass percentage concentration of 75% for 30 seconds; then washed 3 times with sterile water, each time rinsing 35 seconds; then rinse 4 times with sterile water containing sodium hypochlorite, each time for 30 seconds, and finally remove the moisture from the epidermis of the fertilized ovary with sterile filter paper; the mass percentage concentration of sodium hypochlorite in the sterile water containing sodium hypochlorite 1%. In...

Embodiment 2

[0023] The method for in vitro culture of Lycoris long tube seed described in the present embodiment, comprises the following steps:

[0024] Step 1. Selection and disinfection of the fertilized ovary: select the blooming long-tube Lycoris and remove the perianth and receptacle of the long-tube Lycoris, and only keep the complete fertilized ovary; wash the complete fertilized ovary After cleaning, place it on an ultra-clean workbench for sterilization: rinse with alcohol with a concentration of 73% by mass for 35 seconds; then use sterile water to wash 5 times, each time for 30 seconds; then rinse with sterile water containing sodium hypochlorite 4 times, rinse for 20 seconds each time, and finally use sterile filter paper to remove the moisture from the epidermis of the fertilized cell; the mass percentage concentration of sodium hypochlorite in the sterile water containing sodium hypochlorite is 1.2%. In this embodiment, the method for washing the epidermis of the fertilized...

Embodiment 3

[0029] The method for in vitro culture of Lycoris long tube seed described in the present embodiment, comprises the following steps:

[0030]Step 1. Selection and disinfection of the fertilized ovary: select the blooming long-tube Lycoris and remove the perianth and receptacle of the long-tube Lycoris, and only keep the complete fertilized ovary; wash the complete fertilized ovary After cleaning, place it on an ultra-clean workbench for sterilization: rinse with alcohol with a mass percentage concentration of 77% for 25 seconds; then use sterile water to wash 4 times, each time for 25 seconds; then rinse with sterile water containing sodium hypochlorite 5 times, rinse for 28 seconds each time, and finally use sterile filter paper to remove the water from the epidermis of the fertilized cell; the mass percentage concentration of sodium hypochlorite in the sterile water containing sodium hypochlorite is 0.8%. In this embodiment, the method for washing the epidermis of the fertil...

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PUM

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Abstract

The invention discloses a method for in vitro cultivation of Amaryllis long tube seeds, which comprises the following steps: Step 1, selection and disinfection of the fertilized ovary: retaining the complete fertilized ovary of the blooming Lycoris long tube; removing the fertilized ovary Wash the epidermis and put it on the ultra-clean workbench for sterilization; step 2, in vitro culture of the fertilized ovary: put the sterilized fertilized ovary into the first medium for cultivation until the fertilized ovary develops to fullness The capsule of step 3, the in vitro culture of seed: put the full capsule into the second culture medium and continue to cultivate until the seed matures; step 4, the germination of seed: remove the seed coat of mature seed, then remove the seed coat The seeds are put into the third culture medium and cultivated until the seeds germinate; the advantages of the present invention are: the fertilized ovary can be cultivated into mature seeds and seedlings in vitro, thereby greatly improving the reproduction rate of Lycoris long tube, and can also effectively improve the The success rate of edge hybridization.

Description

technical field [0001] The invention relates to the field of in vitro cultivation of plant seeds, in particular to a method for in vitro cultivation of long-tube Lycoris seeds. Background technique [0002] Lycoris long tube belongs to Amaryllidaceae Lycoris. It is a perennial herb with white petals, lily-like flower shape, beautiful flower posture, large color variation and high ornamental value. There are spherical bulbs in the underground of Amaryllis long tube, which contain various alkaloids such as galantamine, licoramine, lycorine, etc., which have certain anticancer activity, anti-inflammatory, antipyretic, sedative and emetic, and can be used for the treatment of Paralysis caused by central paralysis such as Alzheimer's disease and polio, myasthenia gravis and other diseases are important ornamental plants and medicinal plants, so the market demand for this plant is relatively large. [0003] In the natural state, the reproduction of Lycoris is generally divided in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郑玉红邓波张智陈默周坚夏冰
Owner 邓波