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Preparation method of doxorubicin-containing graphene oxide medicine-carrying composite material

A composite material and graphene technology, applied in the direction of medical preparations containing active ingredients, drug combinations, pharmaceutical formulations, etc., can solve problems such as easy agglomeration, potential application value hindrance, etc., achieve high release rate, mild experimental conditions, drug The effect of high loading

Inactive Publication Date: 2014-12-10
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, although graphene oxide is easily soluble in water, it is easy to agglomerate in saline due to the charge shielding effect, which hinders its potential application value in the biomedical field.

Method used

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  • Preparation method of doxorubicin-containing graphene oxide medicine-carrying composite material
  • Preparation method of doxorubicin-containing graphene oxide medicine-carrying composite material
  • Preparation method of doxorubicin-containing graphene oxide medicine-carrying composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Weigh 5mg of graphene oxide composite material, dissolve it in 5ml of water, add 5mL of DOX (4.5mg) aqueous solution dropwise under stirring, adjust the pH to 9.03 with 0.1M sodium hydroxide solution, and put it on aluminum foil The paper was protected from light, and reacted at 37°C for 5h under the condition of a shaking table. Finally, the graphene oxide solution containing doxorubicin was centrifuged at 13,000 rpm for 8 minutes to remove unattached doxorubicin. Finally, the precipitate was freeze-dried to obtain GOX / DOX.

[0030] (2) Weigh 2 mg doxorubicin, prepare respectively 0.002M, 0.004M, 0.008M, 0.016M, 0.032M PBS solution (pH=7.5) and acetic acid solution (pH=5.8), and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0031] (3) Weigh 2.4mg of drug-loaded complex in 2.4ml of phosphate buffe...

Embodiment 2

[0034] (1) Weigh 7.8mg graphene oxide composite material, dissolve it in 8ml water, add 8mL aqueous solution containing DOX (6.2mg) dropwise under the condition of stirring, adjust pH=8.86 with 0.1M sodium hydroxide solution, The aluminum foil was protected from light, and the reaction was carried out at 37°C for 4 hours on a shaker. Finally, the graphene oxide solution containing doxorubicin was centrifuged at 13,000 rpm for 8 minutes to remove unattached doxorubicin. Finally, the precipitate was freeze-dried to obtain GOX / DOX.

[0035] (2) Weigh 2 mg doxorubicin, prepare 0.0025M, 0.005M, 0.01M, 0.02M, 0.04M PBS solution (pH=7.5) and acetic acid solution (pH=5.8) respectively, and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0036] (3) Weigh 4.5mg of the drug-loaded complex in 4.5ml of phosphate buffer (pH...

Embodiment 3

[0039] (1) Weigh 10.3mg of graphene oxide composite material, dissolve it in 12ml of water, add 10mL of DOX (9.8mg) aqueous solution dropwise under stirring, and adjust the pH to 5.5 with 0.1M sodium hydroxide solution , 6.5, 7.5, 8.5 and 9.5, protected from light with aluminum foil, and reacted at 37°C for 5 hours on a shaker. Finally, the graphene oxide solution containing doxorubicin was centrifuged at 13,000 rpm for 8 minutes to remove unattached doxorubicin. Finally, the precipitate was freeze-dried to obtain GOX / DOX.

[0040] (2) Weigh 2 mg doxorubicin, prepare respectively 0.001M, 0.002M, 0.004M, 0.012M, 0.036M PBS solution (pH=7.5) and acetic acid solution (pH=5.8), and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0041] (3) Weigh 0.5 mg of drug-loaded complexes in 1 ml of phosphate buffer (pH=7.5),...

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Abstract

The invention relates to a preparation method of a doxorubicin-containing graphene oxide medicine-carrying composite material. The preparation method comprises the following steps: (1) synthesizing a graphene oxide composite material GOX-PEI-HA; (2) dissolving the graphene oxide composite material into water, adding a doxorubicin hydrochloride aqueous solution, and regulating the pH to be neutral and alkaline; performing light-avoiding oscillating reaction; centrifugally removing disconnected DOX (Doxorubicin); and then freezing and drying to synthesize the GOX (Glucose Oxidase) / DOX medicine-carrying composition. The medicine-carrying material has the characteristic of pH sensitive releasing, can be applied to anti-tumor study, and has a high practical value.

Description

technical field [0001] The invention belongs to the field of preparation of nanometer materials, in particular to a method for preparing a doxorubicin-containing graphene oxide drug-loaded composite material. Background technique [0002] Graphene oxide is the oxide of graphene. Graphene oxide flakes are the product of graphite powder after chemical oxidation and exfoliation. On the one hand, it maintains the layered structure of a single atomic layer of graphene, and can expand to several Ten microns, this structure spans the typical scale of general chemistry and materials science, and has become a hotspot of research. And its research in the field of biomedicine has also risen in recent years. On the other hand, after oxidation treatment, many oxygen functional groups, such as hydroxyl group, carboxyl group, carbonyl group, epoxy group and other hydrophilic groups, are introduced into the graphene sheet of each layer, so it has good aqueous solution stability. and bioco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K31/704A61P35/00
Inventor 朱利民杨卉卉陶磊公晓李赫宇胡娟
Owner DONGHUA UNIV
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