A kind of detection method of black wolfberry

A detection method and technology of black wolfberry, applied in the field of medicine, can solve the problems of high cost, low specificity, time-consuming, etc., and achieve the effect of simple and fast operation, strong specificity, and comprehensive detection

Active Publication Date: 2016-03-02
山东腾贵医药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, there are few detection methods for black wolfberry, which are mainly detected by experience, senses and simple blisters. The specificity is not strong, and it is impossible to make a comprehensive evaluation of its medicinal components, and HPLC-MS is used for identification. Although the test results are accurate, it is time-consuming, laborious and expensive

Method used

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  • A kind of detection method of black wolfberry

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0024] Experimental example 1: Black wolfberry identification experiment

[0025] Test sample: black fruit Lycium barbarum (Jinhe Tibetan Medicine Co., Ltd.).

[0026] Black fruit wolfberry reference substance: provided by Jinhe Tibetan Medicine Co., Ltd., batch number: 20130701.

[0027] Catechin Reference Substance: China Institute for Identification of Pharmaceutical and Biological Products, batch number: 877-200001

[0028] 1. Preparation of the test sample: Take 1g of black wolfberry coarse powder, add 50ml of water, shake and extract, filter, extract the filtrate with ethyl acetate 3 times, 20ml each time, combine the extracts and evaporate to dryness, add 5ml of methanol to dissolve the residue;

[0029] 2. Selection of reference substance: choose wolfberry black fruit as the reference medicinal material, and at the same time choose catechin with the simplest structure among proanthocyanidins as the reference substance to enhance the accuracy and pertinence of identifi...

experiment example 2

[0031] Experimental example 2: Black wolfberry color experiment

[0032] Proanthocyanidins are the main medicinal ingredients in black wolfberry. Many unscrupulous traders use other varieties to pretend to be black wolfberry by dyeing, which does not contain proanthocyanidins at all. Considering that plant pigments may contain biological indicators, the specific color change reaction can be used to easily identify black wolfberry. Through research, it is found that the water extract of black wolfberry is red under acidic conditions (pH 8), which can be used to identify black wolfberry. In the test, hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid, and acetic acid of different concentrations were used as acidic regulators; sodium hydroxide, potassium hydroxide, sodium carbonate, and ammonia were used as alkaline regulators, and it was found that the above regulators were all good The realization of discoloration effect, especially the use of 1mol / L hydrochloric ...

Embodiment 1

[0035] Embodiment 1, a kind of detection method of black wolfberry, comprises following identification method and color developing method:

[0036] Identification method:

[0037] Take 1 g of black wolfberry coarse powder, add 50 ml of water, shake and extract, filter, extract the filtrate with ethyl acetate 3 times, 20 ml each time, combine the extracts and evaporate to dryness, add 5 ml of methanol to the residue to dissolve, and prepare the black wolfberry reference solution in the same way; Take another appropriate amount of catechin control, add methanol to make a reference solution containing 0.2mg per 1ml; operate according to thin-layer chromatography, take 10μl of each of the above three solutions, and spot them on the silica gel G thin-layer plate respectively, and the volume ratio 5:10:1.2:0.5 xylene-ethyl acetate-methanol-formic acid as developing agent, develop, dry, spray with 5% ethanol solution of phosphomolybdic acid by volume, heat until the color is clear, t...

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Abstract

The invention discloses a detection method for Lyciumruthenicum Murr. The Lyciumruthenicum Murr can be accurately detected through a simple and thin-layer chromatography and / or color-developing method. The method is simple and fast, and accurate in results, strong in specificity and simple and fast to operate, and is further more comprehensive in detecting effective components of the LyciumruthenicumMurr, so that needs of an actual test are further met.

Description

technical field [0001] The invention relates to a detection method of black wolfberry, which belongs to the technical field of medicine. Background technique [0002] Black wolfberry (scientific name: Lycium ruthenicum Murr), also known as black fruit wolfberry, is a kind of wolfberry, Tibetan medicine name "Pangma", belongs to the genus Lycium ruthenicum, and is distributed in Northwest China, several provinces in Northwest China, Tibet and Central Asia in Europe. Black wolfberry is one of the special economic plant resources in my country, which has the same nourishing function of medicine and food. Black wolfberry is sweet in taste and flat in nature, rich in protein, wolfberry polysaccharides, amino acids, vitamins, minerals, trace elements and other nutrients. More than blueberry (black wolfberry contains OPC3690mg / 100g; blueberry contains OPC330~3380mg / 100g), it is the natural wild plant with the highest OPC content so far. Proanthocyanidin OPC is the most effective ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N31/16G01N21/79G01N30/90
Inventor 孙绪丁任松鹏赵延霞姬涛魏永义
Owner 山东腾贵医药有限公司
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