Mixed freeze-dried powder for preventing duck viral diseases and preparation method thereof
A viral disease, freeze-dried powder technology, applied in the direction of antiviral agents, resistance to vector-borne diseases, freeze-dried transportation, etc., can solve the problems of poor effect, difficult preservation of drugs, inconvenient operation, etc. The effect of prevention and treatment of duck viral diseases, the effect of strong pertinence
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Embodiment 1
[0038] The mixed freeze-dried powder of anti-duck viral disease of this embodiment is the mixed freeze-dried powder of anti-duck plague, including the following components in parts by weight: 90 parts of anti-duck plague yolk antibody, 2 parts of anti-duck plague transfer factor, α - 2 parts of interferon, 1 part of active lactobacillus, 0.02 part of thimerosal, 0.2 part of formaldehyde, 0.05 part of selenium, 1 part of vitamin C, 1 part of vitamin E, 2 parts of polyvinylpyrrolidone, 0.5 part of glycine.
[0039] The preparation method of the mixed freeze-dried powder of the anti-duck plague of the present embodiment comprises the following steps:
[0040] 1) Propagate the standard strain of duck plague virus, inactivate it to prepare a vaccine, immunize healthy laying ducks every 7 days, and immunize three times in total. For the first immunization, healthy ducks are inoculated with 1.5ml of inactivated vaccine , the second and third immunizations were doubled, and the antibo...
Embodiment 2
[0046] The mixed freeze-dried powder of anti-duck viral disease of the present embodiment is the mixed freeze-dried powder of anti-duck viral hepatitis, comprises the following components by weight: anti-duck viral hepatitis egg yolk antibody 93 parts, anti-duck viral hepatitis 1.5 parts of transfer factor, 1.5 parts of α-interferon, 1 part of active lactobacillus, 0.01 part of thimerosal, 0.3 parts of formaldehyde, 0.1 part of selenium, 0.5 parts of vitamin C, 0.5 parts of vitamin E, 1.5 parts of polyvinylpyrrolidone, 1 part of glycine share.
[0047] The preparation method of the mixed freeze-dried powder of the anti-duck viral hepatitis of the present embodiment comprises the following steps:
[0048] 1) Propagate the standard strain of duck viral hepatitis virus, inactivate it to prepare a vaccine, immunize healthy laying ducks every 7 days, and immunize three times in total, and inoculate the inactivated vaccine for the first time 1.5ml, double the amount for the second ...
experiment example 1
[0054] In this experimental example, performance testing was performed on the mixed freeze-dried powder obtained in Examples 1-2, and the results are shown in Tables 1 and 2.
[0055] Test method for potency detection at room temperature: store the mixed freeze-dried powder preparations obtained in Examples 1 to 2 in a 37°C incubator for 24 months, at 1 week, 2 weeks, January, February, April, and 8 months respectively After 1 month, 12 months, 16 months, 20 months, and 24 months, use the agar diffusion (AGP) method to measure the change of yolk antibody titer in the samples taken at each time point.
[0056] Table 1 embodiment 1~2 gained mixed freeze-dried powder room temperature potency detection experimental result
[0057] group
1 week
2 weeks
January
February
April
August
December
16 months
20 months
24 months
Example 1
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