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Marine microbial stachybotrys longispora and fibrinolytic active compound produced by marine microbial stachybotrys longispora

A technology of Botrytis longosporus and marine microorganisms, applied in the field of marine microorganisms, can solve the problems of high price, short half-life, non-specificity of fibrin, etc.

Inactive Publication Date: 2014-12-17
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently used thrombolytic drugs such as streptokinase (Streptokinase, SK), urokinase (Uropkinase, UK) and tissue-type plasminogen activator (Tissue-type plasminogen activator, t-PA) have short half-lives and are easy to produce Re-embolization, expensive and other problems, and they are not specific to fibrin, often accompanied by bleeding risk while producing thrombolysis

Method used

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  • Marine microbial stachybotrys longispora and fibrinolytic active compound produced by marine microbial stachybotrys longispora
  • Marine microbial stachybotrys longispora and fibrinolytic active compound produced by marine microbial stachybotrys longispora
  • Marine microbial stachybotrys longispora and fibrinolytic active compound produced by marine microbial stachybotrys longispora

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Embodiment 1

[0027] 1. Strain isolation: Potato sucrose medium containing 1% agar, prepared with seawater, with natural pH, sterilized at 121°C for 20 minutes, for example plates, after cooling, spread each plate with a sample volume of 200 μl, and incubate at 25°C for 5 sky.

[0028] 2. The isolated single colony is stored in the potato sucrose slant medium.

[0029] 3. Preparation of fermentation medium: 50g of sucrose, 3g of sodium nitrate, 0.1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 1g of yeast extract, 0.0025g of cobalt chloride, 0.015g of ferrous sulfate, Calcium chloride 0.0065g, distilled water 1000mL, pH5.8.

[0030] 4. Strain culture: culture temperature is 25°C, 180 rpm, after 3 days of culture, add 1% lysine of the culture solution volume. Culture was continued for 2 days.

[0031] 5. Extraction of active substances: add an equal volume of pure methanol, ultrasonically extract for 15 minutes, centrifuge at 10,000 rpm for 15 ...

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Abstract

The invention relates to marine microbial stachybotrys longispora FG216. The marine microbial stachybotrys longispora FG216 is separated from ocean and produces a fibrinolytic active compound FGFC1. The marine microbial stachybotrys longispora FG216 is preserved in the China center for type culture collection (CCTCC) and has an accession number of CCTCC: M2012227. The invention also discloses the fibrinolytic active compound produced by the marine microbial stachybotrys longispora FG216. The fibrinolytic active compound FGFC1 can greatly promote plasmin cellulolytic activity, has excellent druggability and has a further research and application prospect in the medicine industry.

Description

technical field [0001] The invention relates to the technical field of marine microorganisms, in particular to a strain of the marine microorganism Staphylococcus longosporus and a fibrinolytic active compound produced by the marine microorganism Staphylococcus longosa strain. Background technique [0002] Thrombotic diseases, such as acute myocardial infarction, cerebral thrombosis, pulmonary thrombosis, etc., are one of the major diseases that seriously threaten human health, even life-threatening. In developed countries, the diseases and their complications caused by blood clots are the first diseases that account for the death rate of the population. At present, thrombolytic therapy is a routine treatment for myocardial infarction and other thrombotic diseases. Therefore, research on thrombolytic drugs has been highly valued internationally. Currently used thrombolytic drugs such as streptokinase (Streptokinase, SK), urokinase (Uropkinase, UK) and tissue-type plasminoge...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02C12R1/645
CPCC12P1/02C12N1/145C12R2001/645
Inventor 吴文惠包斌陈佳捷严婷孔婷苏同伟张艳王幸
Owner SHANGHAI OCEAN UNIV
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