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Preparation of immunizing antigen and envelope antigen for detecting nitrofurantoin metabolites

A technology for nitrofurantoin metabolites and immune antigens, applied in the field of detection of nitrofurantoin metabolites, can solve problems such as metabolite residues, and achieve the effects of improving immunogenicity, specificity, and coupling ratio

Inactive Publication Date: 2014-12-17
佛山市质量计量监督检测中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies have shown that the half-life of the original drug is usually only a few hours, while its metabolites can still remain in animal tissues for several weeks

Method used

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  • Preparation of immunizing antigen and envelope antigen for detecting nitrofurantoin metabolites
  • Preparation of immunizing antigen and envelope antigen for detecting nitrofurantoin metabolites
  • Preparation of immunizing antigen and envelope antigen for detecting nitrofurantoin metabolites

Examples

Experimental program
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Embodiment Construction

[0020] 1 Synthesis of nitrofurantoin metabolite hapten by acetal method

[0021] 200 mL of ethanol was added into a 500 mL round bottom flask, nitrofurantoin (10.2 g, 0.1 mol) and 6-oxohexanoic acid (15.6 g, 0.12 mol) were added in sequence, and heated to reflux for 3 hours. Heating was stopped, and the reaction solution was naturally cooled to room temperature, and left to stand overnight. After suction filtration, the filter cake was washed with ethanol (20.0 mL×3), and dried at 50° C. for 5 hours to obtain 20.4 g of carboxyl-containing nitrofurantoin derivatives. Preparation of nitrofurantoin metabolite hapten (I).

[0022] 2 Synthesis of Nitrofurantoin Metabolite Immune Antigen by Active Thioester Method

[0023] Dissolve nitrofurantoin hapten (0.214g, 1.0mmol) in 30mL of dimethylformamide (Nimethylformamide, DMF), cool to 0-4°C, add 3-phosphorylated diphenyl ester benzo[D]oxane under stirring Azol-2-one [2(3H)-Benzoxazolone,3-(diphenoxyphosphinyl)-(9CI), DPBO] (0.351g,...

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Abstract

The invention discloses preparation of immunizing antigens and envelope antigens for detecting nitrofurantoin metabolites. The preparation method is characterized by comprising the following steps: by adopting an active thiol ester method, coupling a nitrofurantoin metabolite hapten with a carrier protein, and preparing nitrofurantoin metabolite immunizing antigens and envelope antigens, wherein the nitrofurantoin metabolite hapten is prepared by introducing a nitrofurantoin metabolite into a spacer through an acetal method. According to the optimized coupling technical route, the structure is optimized, and the antibody quality is improved, so that high-sensitivity and high-specificity immunizing antigens and envelope antigens for nitrofurantoin metabolite immune colloidal gold rapid detection are further increased.

Description

technical field [0001] The invention relates to the technical field of detection of nitrofurantoin metabolites in animal-derived foods, in particular to the preparation of immune antigens and coating antigens for detecting nitrofurantoin metabolites. Background technique [0002] Nitrofurantoin is one of the broad-spectrum nitrofuran antibiotics. The basic structure of this type of drug is a furan ring, with a nitro group attached to the fifth carbon atom, which has a strong antibacterial effect. Nitrofurantoin is cheap and effective, and it is widely used in livestock and poultry and aquaculture. Nitrofurantoin is metabolized rapidly in the living body and is completely metabolized in a short period of time. Studies have proved that nitrofurantoin can be metabolized into 1-aminohydantoin (AHD) in animals. AHD can form a binding state with cell membrane proteins in the body, delaying the elimination speed of the drug in the body, so it can remain in the body for several we...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/532G01N33/02G01N33/544
Inventor 潘嘉慧田峻沈国权赵亮亮杨辉朱海云
Owner 佛山市质量计量监督检测中心
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