Flow cytometer multi-wavelength excitation laser cluster method and device

Abstract

The invention provides a flow cytometer multi-wavelength excitation laser cluster method and a device. According to the method and the device, a structure of an apparatus and adjustment can be simplified and optimized, cost is greatly reduced, excitation laser energy loss can be further reduced to a maximum degree, and optical signal intensity of a to be measured cell is improved. The flow cytometer multi-wavelength excitation laser cluster method is characterized in that: excited multi-wavelength excitation laser of multiple laser devices after reverse dispersion and focusing through a dispersion focusing device forms a mixed-wavelength single light beam.

Description

technical field [0001] The invention relates to a flow cytometer used for medical detection instruments, in particular to a method and device for clustering multi-wavelength excitation lasers of the flow cytometer. Background technique [0002] The multi-wavelength excitation laser generating device of the flow cytometer includes multiple lasers of different wavelengths, each laser is arranged in parallel, and emits laser beams of different wavelengths in parallel but dispersed in one direction, and the laser beams of different wavelengths are bundled by the beam The device is integrated into a single beam of mixed wavelengths. The traditional beam converging device is composed of multiple optical filters, beam splitters and focusing lenses. The structure is complex, debugging is difficult, and the energy loss of the excitation laser is large; One by one through the detection sensitive area where the beam converges, due to the fluctuation of fluid focus, it is impossible for...

AI Technical Summary

Problems solved by technology

[0002] The multi-wavelength excitation laser generating device of the flow cytometer includes multiple lasers with different wavelengths, each laser is arranged in parallel, and parallel to one direction but dispersed The laser beams of different wavelengths are emitted, and the laser beams of different wavelengths are integrated into a single beam of mixed wavelengths by the beam concentrator. The traditional beam concentrator is composed of multiple optical filters, beam splitters and focusing lenses. The structure is complex and difficult to debug. The energy loss of the excitation laser is large; and in the traditional flow cytometer, the particles pass through the detection sensitive area where the beam converges one by one. Due to the fluctuation of the fluid focus, it is impossible for the cell particles to pass accurately along the central axis of the microchannel of the flow cytometer. There is a deviation of ±5 μm relative to the central axis of the microchannel. At the same time, the focused spot obtained by the traditional beam converging device has a Gaussian beam energy distribution profile, and the laser intensity located in the profile of the focused spot and interacting with cell particles at different positions It is not a constant, resulting in different optical signals generated by the same kind of cells, and the detection accuracy is low; moreover, the spot diameter of the beam converging on the microchannel of the flow cytometry box is smaller than the axial distance of the microchannel, that is, the detection sensitive area is the microchannel In a specific small section of the axial direction, the cell interacts with the laser for a short time, limited by the response time of the detector to the light signal, the detector cannot accurately detect the light signal with a narrow time width, and the detection accuracy is low

Images