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Biocidal composition of chitosan and Bacillus amyloliquefaciens and application thereof

A technology of Bacillus amyloliquefaciens and amyloliquefaciens, which is applied in the field of pesticides, can solve the problems of excessive pesticide residues, pollute the ecological environment of orchards, and affect the quality of fruit products, and achieve the effects of increasing drug efficacy, high-efficiency bacteriostatic effect, and reducing the amount of use.

Active Publication Date: 2017-01-04
ANHUI FENGLE AGROCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Purpose of the invention: Aiming at the problems that a large number of chemical pesticides are used in the current production to prevent and control grape powdery mildew, strawberry blight (green) wilt, etc., seriously pollute the ecological environment of the orchard, cause pesticide residues to exceed the standard and affect the quality of the fruit, the first purpose of the present invention is to provide A biocidal composition of chitosan and Bacillus amyloliquefaciens with good control effects on grape powdery mildew and strawberry wilt (green) wilt

Method used

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  • Biocidal composition of chitosan and Bacillus amyloliquefaciens and application thereof
  • Biocidal composition of chitosan and Bacillus amyloliquefaciens and application thereof
  • Biocidal composition of chitosan and Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of 5% chitosan·20 billion cfu / gram Bacillus amyloliquefaciens wettable powder

[0023] Drugs for testing:

[0024] 99% chitosan raw material, provided by Henan Shangqiu Kangmeida Biotechnology Co., Ltd.;

[0025] 100 billion cfu / gram Bacillus amyloliquefaciens parent drug is processed and provided by Wuhan Kono Biotechnology Co., Ltd.;

[0026] Additive: NNO (sodium methylene dinaphthalene sulfonate);

[0027] Filling accessories: light calcium carbonate.

[0028] Each component is prepared according to weight percentage:

[0029] 99% chitosan original drug: 5.05%; 100 billion cfu / g Bacillus amyloliquefaciens master drug: 20%; auxiliary NNO: 9%; light calcium carbonate: balance, make up 100%.

[0030] Steps: Put chitosan and Bacillus amyloliquefaciens in the mixer according to the above ratio, add NNO, fill it with light calcium carbonate to 100%, mix all the materials in the mixer evenly, and pulverize twice through the jet mill , Serve as 0....

Embodiment 2

[0031] Example 2 Preparation of 10% chitosan · 20 billion cfu / gram suspension of Bacillus amyloliquefaciens

[0032] Drugs for testing:

[0033] 99% chitosan raw material, provided by Henan Shangqiu Kangmeida Biotechnology Co., Ltd.;

[0034] 100 billion cfu / gram Bacillus amyloliquefaciens parent drug, processed and provided by Wuhan Kono Biotechnology Co., Ltd.;

[0035] Additives: Sodium Lignosulfonate, OP-10; CMC; Organic Silicone; Ethylene Glycol;

[0036] Filling accessories: water.

[0037] Each component is batched according to weight percentage:

[0038] 99% chitosan technical: 10.1%; 100 billion cfu / g Bacillus amyloliquefaciens master drug: 20%; sodium lignosulfonate: 6%; OP-10: 2%; thickener CMC: 1% ; Antifoaming agent organosilicone: 0.03%; Antifreeze agent ethylene glycol: 4%; Water: balance, make up 100%.

[0039] Steps: Put chitosan, Bacillus amyloliquefaciens, sodium lignosulfonate, OP-10, thickener CMC, defoamer organic silicone, antifreeze ethylene glycol i...

Embodiment 3

[0040] Example 3 Preparation of 0.2% chitosan·50 billion cfu / gram Bacillus amyloliquefaciens wettable powder

[0041] Each component is prepared according to weight percentage:

[0042] 99% chitosan original drug: 0.202%; 100 billion cfu / g Bacillus amyloliquefaciens master drug: 50%; auxiliary NNO: 20%; light calcium carbonate: balance, make up 100%.

[0043] Steps: Put chitosan and Bacillus amyloliquefaciens in the mixer according to the above ratio, add NNO, fill it with light calcium carbonate to 100%, mix all the materials in the mixer evenly, and pulverize twice through the jet mill , Serve as 0.02% chitosan · 20 billion cfu / gram wettable powder of Bacillus amyloliquefaciens.

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Abstract

The invention discloses a biological bactericidal composition of chitosan and Bacillus amyloliquefaciens. The biological bactericidal composition comprises effective active ingredients, auxiliary agents and filler materials, and the effective active ingredients account for the weight percentage of the biological bactericidal composition It is 15-60%, and the effective active ingredient is composed of chitosan and Bacillus amyloliquefaciens. The invention also discloses the application of the biocidal composition. In the invention, the bacillus amyloliquefaciens and chitosan are compounded and used in a certain proportion, which can fully exert the coordination function of microorganisms and biological agents, achieve complementary advantages, and improve the prevention and control effect.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and relates to a biological compound preparation, in particular to a biocidal composition of chitosan and Bacillus amyloliquefaciens and its application in the prevention and treatment of grape powdery mildew and strawberry blight (green wilt) . Background technique [0002] In the fruit production of the world, the output and cultivation area of ​​grapes have always been in the first place. In addition to being eaten fresh, grapes are mainly used for wine making, and can also be made into processed products such as grape juice, raisins and canned food. Grapes are not only delicious, but also have high nutritional value. Ripe berries contain 15% to 25% glucose and fructose, as well as a variety of minerals and vitamins beneficial to the human body, so they are loved by people all over the world. Grape has the characteristics of high yield, early fruit, strong adaptability and long life, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P3/00A01N43/16
Inventor 吉沐祥姚克兵庄义庆朱成刚肖婷吴祥
Owner ANHUI FENGLE AGROCHEM