Pleurotus nebrodensis liquid strain culture medium and propagation method of strain

A technology of liquid strain and Bailing mushroom is applied in mushroom cultivation, botanical equipment and methods, cultivation and other directions, which can solve the problems of small scale and dispersion, and achieve the effects of high yield, lower production cost and fast growth.

Inactive Publication Date: 2015-01-07
YONGHE WANGDA MUSHROOM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the actual situation of Bailing mushroom cultivation in my country is small and scattered, and this management mode cannot be fundamentally changed in a short period of time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An edible fungus production cooperative currently has 500L of liquid strain fermentation equipment, and the method of making Bailing mushroom liquid strain culture medium and strain is as follows;

[0024] (1) First weigh 16 kg of bran, 10 kg of corn flour, 4.8 kg of bean cake powder, 8 kg of glucose, 0.4 kg of peptone, and 0.48 kg of KH 2 PO 4 , 0.2 kg of MgSO 4 , 0.04 kg of VB 1 According to the size of the weight, add 400L water in order from less to more during stirring, adjust the pH value of the mixed solution to 6, then transfer it to the liquid strain fermenter, and sterilize it. When the sterilized medium liquid is cooled to After room temperature, the purchased solid seed of Bailing mushroom is inserted, then the culture liquid is adjusted to 24-25°C, the ventilation pressure is ≥0.3Kg / cm, and the strain of Bailing mushroom liquid culture is obtained by fermenting and culturing for 7 days.

[0025] (2) While preparing the liquid culture strains, do a good j...

Embodiment 2

[0027] A farmer has 100L of liquid strain fermentation equipment, and the method of making Bailing mushroom liquid strain culture medium and strain is as follows;

[0028] (1) First weigh 4.5 kg of bran, 2 kg of corn flour, 1.6 kg of bean cake powder, 1.5 kg of glucose, 0.12 kg of peptone, and 0.1 kg of KH 2 PO 4 , 0.06 kg of MgSO 4 , 0.01 kg of VB 1 According to the size of the weight, add 100L water in order from less to more during stirring, adjust the pH value of the mixed solution to 6.5, then transfer it to the liquid strain fermentation tank, and sterilize it. When the sterilized medium liquid is cooled to After room temperature, insert the purchased solid seed of Bailing mushroom, then adjust the culture liquid to 24-25°C, the ventilation pressure is ≥0.3Kg / cm, and ferment and cultivate for 8 days to obtain the strain of Bailing mushroom liquid culture.

[0029] (2) While preparing the liquid culture strains, do a good job in the preparation of the solid bacteria ba...

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PUM

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Abstract

The invention discloses a pleurotus nebrodensis liquid strain culture medium, comprising the following raw materials in parts by weight: 4-4.5 parts of bran, 2-2.5 parts of corn flour, 1.2-1.6 parts of soybean cake powder, 1.5-2 parts of glucose, 0.1-0.12 part of peptone, 0.1-0.12 part of KH2PO4, 0.05-0.06 part of MgSO4 and 0.01 part of VB1 in 100 parts of water. A propagation method of a pleurotus nebrodensis strain comprises the following steps: preparing a liquid culture medium; adjusting the pH value of the liquid culture medium; sterilizing, and inoculating solid strain seeds; cultivating and preparing a solid fungus bag medium; cultivating solid strains; and inoculating and cultivating a fruiting fungus bag. The liquid culture medium disclosed by the invention is abundant in raw material source, and low in price; and due to a solid-liquid-solid production mode, compared with a traditional complete solid culture method, hyphae are even in growth speed in the fungus bag, regular and consistent in fruiting, even in sporocarp size, good in mushroom shape, good in quality, short in fermentation cycle, high in yield, and high in economic benefits, and thus the method is very suitable for popularization in small and medium-sized enterprises and peasant households.

Description

technical field [0001] The invention relates to the technical field of strain culture medium and strain expansion propagation, in particular to a high-efficiency and less-polluting liquid strain culture medium of Bailing mushroom and a method for the expansion propagation of strains. Background technique [0002] Bailing mushroom is a kind of edible fungus with both medicine and food and high nutritional value. The medical value of Bailing mushroom has the functions of eliminating accumulation, killing insects, antitussive, anti-inflammatory and preventing gynecological tumors. According to the determination, the protein content of Bailing mushroom accounts for 20% of dried mushrooms, and contains 17 kinds of amino acids, various vitamins, inorganic salts, fungal polysaccharides, vitamins and other physiologically active substances and various minerals, which can regulate the physiological balance of the human body and strengthen the human body. The role of immune function....

Claims

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Application Information

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IPC IPC(8): C05G1/00A01G1/04
CPCC05B7/00A01G18/00C05D5/00C05F5/002C05F11/00C05F11/10
Inventor 刘三平
Owner YONGHE WANGDA MUSHROOM
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