Pleurotus nebrodensis liquid strain culture medium and propagation method of strain
A technology of liquid strain and Bailing mushroom is applied in mushroom cultivation, botanical equipment and methods, cultivation and other directions, which can solve the problems of small scale and dispersion, and achieve the effects of high yield, lower production cost and fast growth.
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Embodiment 1
[0023] An edible fungus production cooperative currently has 500L of liquid strain fermentation equipment, and the method of making Bailing mushroom liquid strain culture medium and strain is as follows;
[0024] (1) First weigh 16 kg of bran, 10 kg of corn flour, 4.8 kg of bean cake powder, 8 kg of glucose, 0.4 kg of peptone, and 0.48 kg of KH 2 PO 4 , 0.2 kg of MgSO 4 , 0.04 kg of VB 1 According to the size of the weight, add 400L water in order from less to more during stirring, adjust the pH value of the mixed solution to 6, then transfer it to the liquid strain fermenter, and sterilize it. When the sterilized medium liquid is cooled to After room temperature, the purchased solid seed of Bailing mushroom is inserted, then the culture liquid is adjusted to 24-25°C, the ventilation pressure is ≥0.3Kg / cm, and the strain of Bailing mushroom liquid culture is obtained by fermenting and culturing for 7 days.
[0025] (2) While preparing the liquid culture strains, do a good j...
Embodiment 2
[0027] A farmer has 100L of liquid strain fermentation equipment, and the method of making Bailing mushroom liquid strain culture medium and strain is as follows;
[0028] (1) First weigh 4.5 kg of bran, 2 kg of corn flour, 1.6 kg of bean cake powder, 1.5 kg of glucose, 0.12 kg of peptone, and 0.1 kg of KH 2 PO 4 , 0.06 kg of MgSO 4 , 0.01 kg of VB 1 According to the size of the weight, add 100L water in order from less to more during stirring, adjust the pH value of the mixed solution to 6.5, then transfer it to the liquid strain fermentation tank, and sterilize it. When the sterilized medium liquid is cooled to After room temperature, insert the purchased solid seed of Bailing mushroom, then adjust the culture liquid to 24-25°C, the ventilation pressure is ≥0.3Kg / cm, and ferment and cultivate for 8 days to obtain the strain of Bailing mushroom liquid culture.
[0029] (2) While preparing the liquid culture strains, do a good job in the preparation of the solid bacteria ba...
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