Method for extracting glutathione from wheat germs
A glutathione and wheat germ technology, applied in the direction of peptides, etc., can solve the problems of lack of glutathione extraction and inability to make full use of wheat germ resources, and achieve the effects of easy promotion and application, improved competitiveness, and simple operation
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Embodiment 1
[0026] 1) Weigh 10kg of wheat germ and put it in a stirring tank, add 50L of water, stir and extract at 30°C for 20 minutes, centrifuge at 10,000g for 15 minutes after the extraction is completed, and collect the supernatant and precipitate respectively to obtain 38.6L of supernatant and 19.2L of precipitate kg.
[0027] 2) Put 19.2kg of precipitate and 76.8L of water collected in step 1) in a container, stir and leach for 30 minutes at 30°C, centrifuge at 10,000g for 15 minutes after extraction, and obtain 75.7L of supernatant and 20.2kg of precipitate.
[0028] 3) Merge the supernatants obtained in steps 2) and 3), and import into a 5L metal chelate chromatography column. After the feeding is completed, rinse with 10L of water, then elute with 30% ethanol solution, and collect 8.6L of the eluate .
[0029] 4) The eluate collected in step 3) was subjected to vacuum distillation, and the evaporated ethanol was recovered to obtain 1.2 L of concentrated solution, which was free...
Embodiment 2
[0031] 1) Weigh 10kg of wheat germ and place it in a stirring tank, add 100L of water, stir and extract at 80°C for 30 minutes, centrifuge at 10,000g for 15 minutes after the extraction is completed, and collect the supernatant and precipitate respectively to obtain 89.7L of supernatant and 19.5L of precipitate kg.
[0032] 2) Put 19.5kg of precipitate and 156L of water collected in step 1) in a container, stir and extract at 60°C for 60 minutes, centrifuge at 10,000g for 15 minutes after extraction, and obtain 151.3L of supernatant and 21.2kg of precipitate.
[0033] 3) Merge the supernatants obtained in steps 2) and 3) and introduce them into a 5L metal chelate chromatography column. After the feed is completed, rinse with 10L of water, then elute with 50% ethanol solution, and collect 9.1L of eluate .
[0034] 4) The eluate collected in step 3) was subjected to vacuum distillation, and the evaporated ethanol was recovered to obtain 1.3 L of concentrated solution, which was...
Embodiment 3
[0036] 1) Weigh 10kg of wheat germ and put it in a stirring tank, add 40L of water, stir and extract at 30°C for 10 minutes, centrifuge at 10000g for 15 minutes after the extraction is completed, and collect the supernatant and precipitate respectively to obtain 38.7L of supernatant and 19.3L of precipitate kg.
[0037] 2) Place 19.3kg of precipitate and 77.2L of water collected in step 1) in a container, stir and extract at 30°C for 20 minutes, centrifuge at 10,000g for 15 minutes after extraction, and obtain 74.9L of supernatant and 20.1kg of precipitate.
[0038] 3) Combine the supernatants obtained in steps 2) and 3), and import into a 5L metal chelate chromatography column. After the feed is completed, rinse with 10L of water, then elute with 20% ethanol solution, and collect 8.8L of the eluate .
[0039] 4) The eluate collected in step 3) was subjected to vacuum distillation, and the evaporated ethanol was recovered to obtain 1.1 L of concentrated solution, which was fr...
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