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Method for extracting glutathione from wheat germs

A glutathione and wheat germ technology, applied in the direction of peptides, etc., can solve the problems of lack of glutathione extraction and inability to make full use of wheat germ resources, and achieve the effects of easy promotion and application, improved competitiveness, and simple operation

Inactive Publication Date: 2015-01-07
HEBEI ZIFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lack of methods for extracting glutathione from wheat germ, unable to make full use of wheat germ resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Weigh 10kg of wheat germ and put it in a stirring tank, add 50L of water, stir and extract at 30°C for 20 minutes, centrifuge at 10,000g for 15 minutes after the extraction is completed, and collect the supernatant and precipitate respectively to obtain 38.6L of supernatant and 19.2L of precipitate kg.

[0027] 2) Put 19.2kg of precipitate and 76.8L of water collected in step 1) in a container, stir and leach for 30 minutes at 30°C, centrifuge at 10,000g for 15 minutes after extraction, and obtain 75.7L of supernatant and 20.2kg of precipitate.

[0028] 3) Merge the supernatants obtained in steps 2) and 3), and import into a 5L metal chelate chromatography column. After the feeding is completed, rinse with 10L of water, then elute with 30% ethanol solution, and collect 8.6L of the eluate .

[0029] 4) The eluate collected in step 3) was subjected to vacuum distillation, and the evaporated ethanol was recovered to obtain 1.2 L of concentrated solution, which was free...

Embodiment 2

[0031] 1) Weigh 10kg of wheat germ and place it in a stirring tank, add 100L of water, stir and extract at 80°C for 30 minutes, centrifuge at 10,000g for 15 minutes after the extraction is completed, and collect the supernatant and precipitate respectively to obtain 89.7L of supernatant and 19.5L of precipitate kg.

[0032] 2) Put 19.5kg of precipitate and 156L of water collected in step 1) in a container, stir and extract at 60°C for 60 minutes, centrifuge at 10,000g for 15 minutes after extraction, and obtain 151.3L of supernatant and 21.2kg of precipitate.

[0033] 3) Merge the supernatants obtained in steps 2) and 3) and introduce them into a 5L metal chelate chromatography column. After the feed is completed, rinse with 10L of water, then elute with 50% ethanol solution, and collect 9.1L of eluate .

[0034] 4) The eluate collected in step 3) was subjected to vacuum distillation, and the evaporated ethanol was recovered to obtain 1.3 L of concentrated solution, which was...

Embodiment 3

[0036] 1) Weigh 10kg of wheat germ and put it in a stirring tank, add 40L of water, stir and extract at 30°C for 10 minutes, centrifuge at 10000g for 15 minutes after the extraction is completed, and collect the supernatant and precipitate respectively to obtain 38.7L of supernatant and 19.3L of precipitate kg.

[0037] 2) Place 19.3kg of precipitate and 77.2L of water collected in step 1) in a container, stir and extract at 30°C for 20 minutes, centrifuge at 10,000g for 15 minutes after extraction, and obtain 74.9L of supernatant and 20.1kg of precipitate.

[0038] 3) Combine the supernatants obtained in steps 2) and 3), and import into a 5L metal chelate chromatography column. After the feed is completed, rinse with 10L of water, then elute with 20% ethanol solution, and collect 8.8L of the eluate .

[0039] 4) The eluate collected in step 3) was subjected to vacuum distillation, and the evaporated ethanol was recovered to obtain 1.1 L of concentrated solution, which was fr...

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Abstract

The invention provides a method for extracting glutathione from wheat germs. The method is characterized by comprising the following steps: (1) first extraction, wherein water is added to the wheat germs for leaching, and then solid-liquid separation is performed; (2) second extraction, wherein water is added to solids separated in the step (1) for leaching, and then the solid-liquid separation is performed; (3) the separation of the glutathione, wherein liquid separated in the step (1) and liquid separated in the step (2) are merged, through metal chelated affinity chromatography mediums, elution buffer solution is used for elution, and eluent is collected; (4) concentration and drying, wherein the eluent collected in the step (3) is concentrated and dried to obtain the glutathione. The method has the advantages of stable technology, simple and convenient operation, standardized equipment, low cost, no pollution and convenient popularization and application.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a method for extracting glutathione from wheat germ Background technique [0002] Glutathione (GSH) is a tripeptide composed of glutamic acid, cysteine ​​and glycine, which can help maintain normal immune system function, and has antioxidant and integrated detoxification effects. Glutathione can not only eliminate free radicals in the human body, but also improve human immunity. Glutathione can also protect hemoglobin from hydrogen peroxide oxidation, free radicals and other oxidations so that it can continue to function normally to transport oxygen. Glutathione has a broad-spectrum detoxification effect. It can be used not only in medicine, but also as a base material for functional foods. It is widely used in functional foods such as delaying aging, enhancing immunity, and anti-tumor. [0003] At present, glutathione is mainly obtained through microbial fermentation or a...

Claims

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Application Information

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IPC IPC(8): C07K5/037C07K1/36C07K1/30C07K1/22
Inventor 孔英俊杨子丰张贵锋马啸宇张泽玉陈静康跻耀
Owner HEBEI ZIFENG BIOTECH
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