Semi-continuous culture method for spores recovered from cordyceps sinensis fermentation liquor

Abstract

The invention belongs to the technical field of cordyceps sinensis fermental cultivation, and particularly relates to a semi-continuous culture method for spores recovered from a cordyceps sinensis fermentation liquor. The semi-continuous culture method comprises the steps of preparing a semi-continuous fermentation tank and a culture medium, detecting fermentation, concentrating mycelia, separating the fermentation liquor from spores and continuing fermental cultivation. Mycelia entering a filtrate are reduced by selecting a membrane filter during filtration and even selecting a selective membrane for filtration, so that the yield of mycelia is improved, the COD of the filtrate is reduced, the sewage treatment capacity is lowered, cells are not destroyed, and the total recovery of effective components in the cells is guaranteed; a selective membrane filter system has a good effect in separating mycelia from spores, the quantity of separated cordyceps sinensis spores is relatively large, and environmental transformation is mild, so that the adaptation time of a seed solution is shortened, the seed solution directly enters the multiplicative fermentation process, and accordingly, the fermentation period is also shortened by about 16 hours; and the production efficiency is improved, the production power consumption is reduced and better benefits are acquired.

Description

technical field [0001] The invention relates to the technical field of fermentation and cultivation of Cordyceps sinensis, in particular to a semi-continuous culture method for recovering spores from fermentation liquid of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a national second-class protected plant. It is mainly distributed in the alpine meadows of Tibet, Qinghai, Sichuan, Yunnan and Gansu. It has the effects of nourishing the kidneys and lungs, nourishing the heart and liver, and enhancing disease resistance. However, due to the particularity of the growth of Cordyceps sinensis, it has become very rare and precious, while the demand for Cordyceps sinensis has doubled at home and abroad, and the price has skyrocketed, prompting people to over-excavate and seriously destroying the natural resources of Cordyceps sinensis. The demand of the market alleviates the destruction of natural resources of Cordyceps sinensis, and the thinking of artifi...

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Problems solved by technology

[0005] For this reason, some researchers have made research on the separation and extraction method of Cordyceps sinensis mycelium and the production method of Cordyceps sinensis mycelium powder from the above technical problems, such as the patent No. CN201210344464. The invention patent of "method of mycelium" discloses that the Cordyceps spores fermentation liquid is separated by microfiltration membrane to obtain the spores of Cordyceps spores, which changes the traditional centrifugation method used for separation of spores of Cordyceps spores; the patent number is CN200810095732.8 " The method of producing Cordyceps sinensis mycelium powder by membrane filtration” discloses that the final bacterial liquid produced by the fermentation method is vacuum-filtered with a drum, and the filtrate obtained is filtered by a membrane filtration method, and the filtered solid is dried and pulverized by microwaves to obtain Cordyceps sinensis fungus Silk powder, the filtered filtrate is concentrated and spray-dried to obtain Cordyceps nutrient powder. This method can effectively recover about 10-25% of Cordyceps mycelia, which reduces a lot of waste, reduces production costs, and improves production efficiency. It has good economic and social benefits; that is, the above prior art has not studied the nutritional protection of the Cordyceps sinensis cultivation process, even if the membrane filtration method is used to replace the traditional centrifugation method, it can avoid the damage of the Cordyceps cell body and exclude part of the nutrients from flowing into the fermentation solution, but its effects are relatively unsatisfactory; and there is no research on the cultivation cycle of Cordyceps sinensis, which makes the cultivation cost of Cordyceps sinensis relatively large and the profit is low

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