Composite microbial seed dressing agent and preparation method and application of composite microbial seed dressing agent

A technology of compound microorganisms and seed dressings, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems that have not been reported, achieve high market value, good compatibility, and easy control of cultivation conditions Effect

Active Publication Date: 2015-01-07
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, PGPR mixed with rhizobia to inoculate non-legume plants, and the spe

Method used

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  • Composite microbial seed dressing agent and preparation method and application of composite microbial seed dressing agent
  • Composite microbial seed dressing agent and preparation method and application of composite microbial seed dressing agent
  • Composite microbial seed dressing agent and preparation method and application of composite microbial seed dressing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Isolation and identification of Bacillus coagulans LN1:

[0040]The applicant isolated, screened, and identified the plant rhizosphere from the experimental farm of Huazhong Agricultural University, and preserved it in the China Center for Type Culture Collection on May 23, 2014. The classification name: Bacillus coagulans LN1, and the preservation number is CCTCC NO: M 2014223.

[0041] Bacillus coagulans LN1 is a rod-shaped cell, forming mesophytic, oval spores, sporangia are not enlarged, colonies are yellow, moist and sticky, raised, with neat edges, Gram staining is positive, catalase test is positive, VP test is positive, VP reaction final pH 4.4, positive starch hydrolysis reaction, positive nitrate reduction reaction, positive citrate utilization reaction, can grow at pH 5.7, cannot grow in 7% NaCl, refer to Bergey’s Bacteria Handbook (Eighth Edition) The bacterium conformed to the physiological and biochemical characteristics of Bacillus coagulans and was iden...

Embodiment 2

[0117] A kind of composite microbial seed dressing, its preparation steps are as follows:

[0118] Preparation of Bacillus coagulans LN1, Bacillus licheniformis LN6, Bacillus pumilus LN7, and Bacillus amyloliquefaciens LN9: inoculate in the seed medium, cultivate at 37 degrees for 12 hours to become the first-grade seed solution, and transfer the first-grade seed solution to In the seed medium, culture at 37 degrees for 12 hours is the second-grade seed liquid. Level 2 seed liquid is inoculated in the fermentation medium at 37 degrees, the inoculum size is 4%, the liquid volume is 25 / 50L, the pressure is 0.05-0.1MPa, the initial speed is 220r / min, the initial pH is 7, and the initial air flow rate is 1L / min, real-time defoaming and control of dissolved oxygen above 30%, the end of fermentation time is 24 hours.

[0119] Seed medium: beef extract peptone medium (beef extract 3.0g, peptone 10.0g, NaCl 5.0g, water 1000ml, pH 7.4-7.6)

[0120] Fermentation medium:

[0121] Bac...

Embodiment 3

[0143] The application of a compound microbial seed dressing agent in terms of crop growth promotion and disease resistance is as follows:

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Abstract

The invention discloses a composite microbial seed dressing agent and a preparation method and an application of the composite microbial seed dressing agent. The composite microbial seed dressing agent is compounded by sinorhizobium fredii, bacillus coagulans, bacillus licheniformis, bacillus pumilus, bacillus amyloliquefaciens and turf. The composite microbial seed dressing agent has the effects of fixing nitrogen, solubilizing phosphorus, dissolving potassium, dissolving cellulose and starch, producing plant growth hormone, resisting diseases and the like, also has the function of stress resistance, and is good in mutual compatibility, easy to control over culture conditions and the like; the composite microbial seed dressing agent can be evenly stirred and sowed together with seeds; and the compound fertilizer is used as a base fertilizer to reduce use of a compound fertilizer; therefore, the composite microbial seed dressing agent has relatively high market value.

Description

technical field [0001] The invention relates to a compound microbial seed dressing agent, in particular to a compound microbial seed dressing agent with growth-promoting and disease-resistant effects on crops and a preparation method thereof, belonging to the technical field of agricultural microbial bacterial agents. Background technique [0002] In the late 1990s, agricultural microbial agents have formed a trend of transformation from a single strain to a composite strain, from a single function to a multi-function, and from non-spores to spores. Existing agricultural microbial agents mainly include: rhizobia and nitrogen-fixing agents, phosphorus-dissolving bacteria (true) agents, silicate agents, growth-promoting agents, organic material (straw) decomposing agents, and actinomycetes agents , photosynthetic bacteria preparations, mycorrhizal fungi preparations, anaerobic preparations, soil (water body) restoration agents, etc. [0003] PGPR (plant growth promoting rhizo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C12R1/07C12R1/01C12R1/10C12R1/08
CPCC05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 李友国陈龙男
Owner HUAZHONG AGRI UNIV
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