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Photoautotrophic root method of sugarcane test tube seedlings

A technology for test-tube seedlings and sugar cane, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of high cost and complicated production process, and achieve the reduction of production cost, improvement of production efficiency, energy saving and labor input. Effect

Active Publication Date: 2016-06-29
广西中科溪岗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the complex production process of traditional sugarcane test-tube plantlets, strict requirements on the cultivation environment, and high cost problems, and provide a method for photosynthetic autotrophic rooting of sugarcane test-tube plantlets

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0030] The photosynthetic autotrophic rooting method for sugarcane test-tube plantlets comprises the following steps:

[0031] (1) Select Xintaitang No. 22 subcultured rootless sugarcane seedlings and place them in the subculture medium containing MS+6-BA1.0mg / L+naphthaleneacetic acid 0.01mg / L+sucrose 20g / L, and put them in a cool, sheltered place place, open the bottle cap, and spray gibberellin (GA) with a weight ratio of 1:2 3 ) (concentration 10mg / L) and indolebutyric acid (concentration 20mg / L) plant growth regulator mixed solution, hardening seedlings for 1 day;

[0032] (2) Take out the test-tube seedlings from the bottle on the second day, wash the culture medium with clear water, soak and disinfect with 0.4% potassium permanganate solution for 6 minutes after drying, wash the potassium permanganate solution with clear water again, and dry it in the air , then soak the test-tube plantlets in an auxin mixture containing 100 mg / L of naphthaleneacetic acid and 100 mg / L o...

Embodiment 2

[0036] The photosynthetic autotrophic rooting method for sugarcane test-tube plantlets comprises the following steps:

[0037] (1) Select Xintaitang No. 22 subcultured rootless sugarcane seedlings and place them in the subculture medium containing MS+6-BA1.0mg / L, naphthaleneacetic acid 0.01mg / L, sucrose 20g / L, and put them in a cool place , in a sheltered place, open the bottle cap, and spray gibberellin (GA) with a weight ratio of 1:5 3 ) (concentration 10mg / L) and indolebutyric acid (concentration 50mg / L) plant growth regulator mixed solution, hardening seedlings for 1 day;

[0038] (2) Take out the test-tube seedlings from the bottle on the second day, wash the culture medium with clear water, soak and disinfect with 0.4% potassium permanganate solution for 6 minutes after drying, wash the potassium permanganate solution with clear water again, and dry it in the air , then soak the test-tube plantlets in an auxin mixture containing 100 mg / L of naphthaleneacetic acid and 10...

Embodiment 3

[0042] The photosynthetic autotrophic rooting method for sugarcane test-tube plantlets comprises the following steps:

[0043](1) Select Xintaitang No. 22 subcultured rootless sugarcane seedlings and place them in the subculture medium containing MS+6-BA1.0mg / L+naphthaleneacetic acid 0.01mg / L+sucrose 20g / L, and put them in a cool, sheltered place place, open the bottle cap, and spray gibberellin (GA) with a weight ratio of 1:10 3 ) (concentration 10mg / L) and indolebutyric acid (concentration 100mg / L) plant growth regulator mixed solution, hardening seedlings for 1 day;

[0044] (2) Take out the test-tube seedlings from the bottle on the second day, wash the culture medium with clear water, soak and disinfect with 0.4% potassium permanganate solution for 6 minutes after drying, wash the potassium permanganate solution with clear water again, and dry it in the air , then soak the test-tube plantlets in an auxin mixture containing 100 mg / L of naphthaleneacetic acid and 100 mg / L ...

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Abstract

The invention relates to a photoautotrophic rooting method for test-tube seedlings of sugarcane. The method is mainly to select subcultured rootless sugarcane tissue-cultured seedlings to open a bottle for hardening, and at the same time spray plant growth-promoting liquid, and take out the test-tube seedlings for cleaning after 2-3 days of hardening , carry out disinfection and auxin solution soaking treatment, and finally divide the test-tube seedlings into single plants or small clusters and plant them in planting pots and put them into the seedling cultivation greenhouse for cultivation and rooting. The photosynthetic autotrophic rooting method of sugarcane test tube seedlings of the present invention saves the traditional procedure of rooting in test tubes of rootless test tube seedlings, replaces traditional heterotrophic rooting with autotrophic rooting, shortens the seedling cultivation period, saves production costs, and is beneficial to the health of sugarcane Large-scale production and promotion of test tube seedlings.

Description

technical field [0001] The invention relates to a photoautotrophic rooting method for plant test-tube seedlings, in particular to a photoautotrophic rooting method for sugarcane test-tube seedlings. Background technique [0002] Plant tissue culture generally goes through four stages, that is, primary culture (or establishment of a sterile culture system), subculture proliferation culture, rooting culture and seedling hardening and transplanting. At present, the sugarcane test-tube seedling rapid breeding technology that has been applied on a large scale in production also includes the above four stages. However, this traditional sugarcane test-tube plantlet production process is complex and requires high environmental conditions for cultivation, resulting in high cost of sugarcane tissue culture industrial production of test-tube plantlets, which has been unacceptable by the market and has limited the cultivation and production of healthy sugarcane seedlings. promote. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 何为中
Owner 广西中科溪岗生物科技有限公司
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