A group of oligonucleotide aptamers that specifically recognize Shigella sonnei

Abstract

The invention discloses a set of oligonucleotide aptamers Sp1 and Sp20 for specifically recognizing Sh.sonnei, belonging to the field of food safety molecular biology. The set of oligonucleotide aptamers Sp1 and Sp20 is prepared through the steps of through a Cell-SELEX technology, sequentially carrying out screening, amplifying, enzyme digestion, sequencing, and affinity and specificity analysis on a random single stranded oligonucleotide library synthesized in vitro by taking a complete Sh.sonnei cell as a target. The aptamers adopted by screening have the characteristics of high affinity and specificity, stable properties, low cost, and the like, is convenient for vitro synthesis and can be applied to the detection of Sh.sonnei in foods by marking functional groups or fluorescent dyes, thereby providing a new choice for existing antibody-dependent detection methods.

Description

technical field [0001] The invention relates to the field of food safety biotechnology, in particular to screening a group of oligokaryotes with high specific affinity for Shigella sonnei using SELEX technology (exponentially enriched ligand system evolution technology) in molecular biology technology The nucleotide aptamer provides scientific and theoretical basis for the application of the nucleic acid aptamer in the detection of Shigella sonnei in food. Background technique [0002] Shigella is a highly infectious and serious Gram-negative enteropathogenic bacterium. In developing countries, major health events of bacillary dysentery caused by Shigella occur frequently, especially in summer and autumn. There are about 160 million infection cases and about 1 million deaths from bacillary dysentery every year. Shigella is mainly caused by oral infection, and salads, raw vegetables, milk and dairy products, poultry, fruits, drinking water, and bakery products are easily con...

AI Technical Summary

Problems solved by technology

Traditional bacterial culture and biochemical identification are time-consuming and cumbersome to operate; although molecular biology detection methods such as PCR are fast and accurate, their specificity is easily affected by operating conditions; immunological detection is characterized by strong specificity, accuracy and sensitivity, but The process of preparing specific antibodies is time-consuming and complicated, and the prepared antibodies are also susceptible to environmental factors such as temperature

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