Method for detecting calcium retention capability of casein phosphopeptides products

A technology of casein phosphopeptide and determination method, which is applied in the field of determination of calcium holding capacity of casein phosphopeptide products, can solve problems such as difficult to meet formula design and low calcium holding capacity, and achieve accurate and reliable results, good reproducibility, The effect of high sensitivity

Inactive Publication Date: 2015-01-21
西安莹朴生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Without adding any auxiliary materials, the amount of calcium carbonate and CPP products with low calcium holding capacity has reached 0.613g, which exceeds the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take a commercially available high-purity CPP product, use Chelex-100 to remove the calcium in the CPP product, and then use deionized water to prepare a CPP solution with a concentration of 100 mg / mL, take 3.75 mL, stir at room temperature for 10 minutes, and then add PH=8.0 Stir 5mL potassium dihydrogen phosphate and potassium hydrogen phosphate buffer solution evenly, then add 1.25mL calcium chloride solution with a concentration of 100mg / mL and mix evenly, let the solution stand at 25°C for 40 minutes until the precipitation is fully produced, then in Centrifuge at 2900rpm for 20 minutes to remove the precipitate in the solution, then the supernatant contains calcium ions complexed with CPP. The calcium content in the supernatant was detected by an atomic absorption spectrometer, and the amount of calcium ions that CPP at this concentration could bind was converted. It has been determined that the calcium holding capacity of the CCP product is 74.7mg / 100mg.

Embodiment 2

[0015] Take a commercially available low-purity CPP product, use Chelex-100 to remove the calcium in the CPP product, and then prepare a CPP solution with a concentration of 100 mg / mL with deionized water, take 3.75 mL, stir at room temperature for 10 minutes, and then add PH=8.0 Stir 5mL potassium dihydrogen phosphate and potassium hydrogen phosphate buffer solution evenly, then add 1.25mL calcium chloride solution with a concentration of 100mg / mL and mix evenly, let the solution stand at 25°C for 40 minutes until the precipitation is fully produced, then in Centrifuge at 2900rpm for 20 minutes to remove the precipitate in the solution, then the supernatant contains calcium ions complexed with CPP. The calcium content in the supernatant was detected by an atomic absorption spectrometer, and the amount of calcium ions that CPP at this concentration could bind was converted. It has been determined that the calcium holding capacity of the CCP product is 19.4mg / 100mg.

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Abstract

The invention relates to a method for detecting calcium retention capability of casein phosphopeptides products, belonging to the field of food additives and detection methods of the food additives. The method for detecting calcium retention capability of casein phosphopeptides product comprises the following steps: removing calcium in CPP products by Chelex-100, adding deionized water and preparing the CPP products into a CPP solution with a certain concentration, stirring at the room temperature for 10 minutes, adding 5mL of a buffer solution prepared from monopotassium phosphate and potassium hydrogen phosphate with a pH value of 8.0 and uniformly stirring, then adding calcium chloride solution and uniformly mixing, standing the solution at 25 DEG C for 40 minutes until sediment is fully generated, subsequently centrifuging for 20 minutes at 2900rpm to remove the sediment in the solution, wherein the supernate contains calcium ions complexed with CPP; detecting the content of calcium in the supernate by virtue of an atomic absorption spectrometer and calculating the quantity of the calcium ions complexed with CPP with the certain concentration. The detection method has the characteristics of being simple and quick, high in sensitivity, high in reproducibility and accurate and reliable in result, therefore, the detection method can be applied to rapid and conventional analysis and detection of the calcium retention capability of casein phosphopeptides products.

Description

technical field [0001] The invention relates to a method for measuring the calcium holding capacity of a casein phosphopeptide product, and belongs to the field of food additives and detection methods thereof. Background technique [0002] Casein phosphopeptide (CPP for short) is an active peptide product obtained from bovine casein through enzymatic hydrolysis, separation and purification. Studies have found that CPP can combine with calcium ions to form a stable soluble complex, thereby preventing or delaying the formation of calcium ions in a neutral or weakly alkaline environment. The ability of CPP to bind calcium is closely related to factors such as the composition and sequence of amino acids in the molecule or the distribution of phosphate groups. Among them, the nitrogen / phosphorus molar ratio (N / P) of CPP products can objectively reflect the physical and chemical properties of CPP products such as purity, phosphate group density, and calcium binding capacity. Gen...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 李勇刘文斌
Owner 西安莹朴生物科技股份有限公司
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