Separation and extraction technology of andrographolide and dehydroandrographolide

Abstract

The invention discloses andrographolide, dehydroandrographolide, and a separation and extraction technology of andrographolide and dehydroandrographolide. The technology comprises the following steps: preparing the dry aboveground parts of a traditional Chinese herbal andrographis paniculata, grinding the aboveground parts, filling the grinded aboveground parts into an extraction pot, using ethanol to extract the aboveground parts for three times, subjecting the liquid andrographis paniculata extracts obtained in the first and second extractions to reduced pressure distillation so as to recycle the ethanol, condensing the extracts, carrying out high-speed centrifugal separation, collecting the centrifugation precipitate, weighing the precipitate, discarding the centrifugate; adding ethanol into the centrifugation precipitate, wherein the weight of the ethanol is 7 times heavier than that of the precipitate, and the concentration of the ethanol is 80%; stirring, filtering, clarifying, making the filtrate, which has been filtered by a ceramic membrane, go through a resin column, which is filled with ion exchange resin, then carrying out elution with ethanol with a concentration of 75%, making the discolored liquid, which has gone through the ion exchange resin, go through a chromatographic column, which is filled with refined auxiliary materials, adding a decoloring agent into an alumina refined liquid, evenly mixing, filtering so as to obtain a discolored liquid; subjecting the discolored liquid to reduced pressure distillation to recycle the ethanol; carrying out step-by-step crystallization and recrystallization by controlling the condensed volume of the discolored liquid and the ethanol degree, purifying the products, carrying out suction filtration and separation so as to obtain crystals; and drying two crystals so as to obtain the finished products. The technology has the advantages of low cost, high product yield, short production period, and capability for massive production.

Description

technical field [0001] The invention relates to biotechnology, in particular to a separation and extraction process of andrographolide and dehydroandrographolide. Background technique [0002] Andrographis paniculata, also known as Yijianxi and Lamane, is an annual herb produced in Guangxi, Guangdong, Hainan and other provinces. The aerial part of Andrographis paniculata mainly contains diterpene lactone compounds andrographolide (andrographolide), andrographolide A (deoxyandrographolide), and andrographolide (neoandrographolide), etc. [0003] Andrographolide and dehydroandrographolide have anti-pathogenic microorganisms, antipyretic, anti-inflammatory, affect the immune function of the body, anti-fertility, gallbladder and liver protection, anti-tumor and other activities. In addition, andrographolide can increase the function of adrenal cortex, and Andrographis A, B, C, and D can shrink the thymus of young mice at larger doses. Clinical application: for bacillary dysent...

AI Technical Summary

Problems solved by technology

[0005] (1) Supercritical extraction and purification technology is adopted, but this technology requires large investment in equipment, high production cost, and small production and processing volume, while the price of andrographolide and dehydroandrographolide is relatively low, less than 2,000 yuan / kg

However, this technology uses a large amount of adsorption chromatographic agents and organic solvents, and the production cost is high, and the organic solvents are toxic, harmful, and volatile, which causes excessive production costs.

At present, there is no domestic company that adopts this technology to produce Andrographis paniculata extract

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