Method for separating and purifying xylanase from waste flammulina velutiper cultivation material

A technology of xylanase and cultivation waste, applied in the field of separation and purification of xylanase, which can solve the problems of waste of organic resources, impact of harsh environment, and ineffective utilization, etc., and achieve the effect of improving enzyme activity

Inactive Publication Date: 2015-01-28
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, my country's edible mushroom cultivation waste resources are huge, which have not been eff

Method used

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  • Method for separating and purifying xylanase from waste flammulina velutiper cultivation material
  • Method for separating and purifying xylanase from waste flammulina velutiper cultivation material
  • Method for separating and purifying xylanase from waste flammulina velutiper cultivation material

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Embodiment 1

[0025] 1 Extraction optimization of xylanase

[0026] 1.1 Single factor optimization

[0027] (1) Optimization of the pH value of the extract

[0028] For the optimization of the pH value of the xylanase extract, select the pH value in the range of 4.0-10.0, set a gradient every 0.4, and set up 16 gradients in total. Each group was added with the same mass of crushed and evenly stirred cultivation waste, respectively added with different pH value extracts, mixed and allowed to stand at room temperature, and three parallels were made for each gradient. Filter through gauze, centrifuge at 10,000 r / min for 20 min at 4°C, and take the supernatant. The xylanase activity of each group of extracts was measured, and the results showed that the xylanase activity gradually increased from pH 4.0, and the xylanase activity reached the highest when the pH value of the extract was 6.0. There are about 61.77U. After that, the xylanase activity gradually decreased with the increase of...

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Abstract

The invention provides a method for separating and purifying xylanase from waste flammulina velutiper cultivation materials. According to the method, digestion conditions of xylanase left in the waste flammulina velutiper cultivation materials are optimized, and methods such as ammonium sulfate precipitation, dialysis, SephadexG-100 sephadex chromatography and DEAEC-52 ion-exchange chromatography are adopted to separate and purify cellulose, and the purity of protein achieves electrophoresis purity by virtue of SDS-PAGE identification. The xylanase is extracted from the waste flammulina velutiper cultivation materials and is effectively separated and purified, the structure and the function of xylanase can be deeply studied, and the scientific reference is provided for production and application of xylanase.

Description

technical field [0001] The invention belongs to the technical field of fungal cultivation waste production, in particular to a method for separating and purifying xylanase from Flammulina velutipes cultivation waste. Background technique [0002] At present, my country's edible mushroom cultivation waste resources are huge, which have not been effectively utilized for a long time, resulting in bad environmental impact and huge waste of organic resources. Edible fungus cultivation wastes contain a large amount of residual extracellular enzymes. These extracellular enzymes have very broad industrial utilization value and application prospects. Shiji mushroom and Flammulina velutipes are the main varieties of industrial production at present. The source of waste fungus bags is stable, which is industrialized. The extraction of cellulase and xylanase provides a stable source of raw materials, and the use of these resources through scientific and effective methods is of great sig...

Claims

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Application Information

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IPC IPC(8): C12N9/42
CPCC12N9/248
Inventor 杨云龙胡开辉黄彩梅万春和
Owner FUJIAN AGRI & FORESTRY UNIV
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