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Method for producing biodiesel by efficiently utilizing corncob residues via Rhodococcus

A technology of biodiesel and Rhodococcus, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as limited land utilization

Inactive Publication Date: 2015-01-28
ZHENJIANG BIO INNOVA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, oil-producing crops are limited by the availability of land, and it is not worth the loss to compete with food for land to produce biodiesel

Method used

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  • Method for producing biodiesel by efficiently utilizing corncob residues via Rhodococcus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Seed medium: glucose 10g / L, yeast extract 5g / L, malt extract 10g / L, Na 2 HPO 4 12H 2 O 9g / L, KH 2 PO 4 1.5g / L. Except for glucose, other components of the medium were sterilized at 121°C for 20 minutes, and glucose was sterilized at 115°C for 20 minutes alone. The inoculum size was 2%, the initial pH was 7.2±0.02, 28°C, 200rpm, and cultivated for 24h.

[0008] OD measured after 24h 600 was 47.2.

Embodiment 2

[0010] Change the amount of nitrogen source in the culture medium, transfer the bacteria in the seed culture tank in the above-mentioned embodiment 1 to the culture medium below to expand the culture respectively.

[0011] Nutritional balance medium: xylose residue 80g / L, urea 10g / L, MgSO 4 ·7H 2 O 1g / L, CaCl 2 2H 2 O 0.02g / L, FeSO 4 ·7H 2 O 0.5g / L, ZnSO 4 ·7H 2 O 0.4g / L, MnSO 4 ·5H 2 O 0.02g / L, H 3 BO 3 0.015g / L, NiCl 2 2H 2 O 0.01g / L, EDTA 0.25g / L, CoCl 2 ·6H 2 O 0.05g / L, CuCl 2 2H 2 O 0.005g / L, Stock A solution 1ml / L, 1.0M phosphate buffer solution 35.2ml / L.

[0012] Nitrogen-limited medium: xylose residue 80g / L, urea 0.636g / L, and other ingredients consistent with the nutrient-balanced medium.

[0013] Stock A solution formula: NaMoO 4 2H 2 O 2g / L, FeNa·EDTA 5g / L.

[0014] 1.0M Phosphate Buffer Solution Formula: KH 2 PO 4 47g / L, K 2 HPO 4 113g / L.

[0015] The seed solution fermented in Example 1 was transferred into a nutrient-balanced medium a...

Embodiment 3

[0016] Embodiment 3: Determination of rhodococcus cell oil production rate

[0017] A. Determination of dry weight of Rhodococcus cells

[0018] After the fermentation in Example 2 above, 1ml of fermentation broth was taken from two fermenters, centrifuged at 10,000rpm for 10min, the biomass precipitate was washed twice with water, and dried at a constant temperature at 40°C for 24h, and the biomass was determined by gravimetric analysis.

[0019] B. Extraction of oil from Rhodococcus cloudy cells (extraction method of Folch oil)

[0020] The Rhodococcus biomass produced by fermentation in the above example was homogenized with 20 volumes of chloroform:methanol (2:1) mixture. After dispersion, the entire mixture was stirred overnight on an orbital shaker at room temperature, centrifuged and the liquid phase recovered. The solvent was washed with 0.2 volumes of water. After vortexing for a few seconds, the two phases were separated by centrifugation at 2000 rpm. The upper p...

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Abstract

The invention provides biodiesel, which has low sulfur content, does not contain aromatic compounds and is an environment-friendly renewable energy source. Rhodococcus has a very strong carbon source conversion capability and can be used for highly efficiently converting lignocellulose which is difficult to utilize in daily life into fatty acid; and the oil accumulation amount of Rhodococcusopacus PD630 can be up to 86% of the dry weight of cells when glucose is used as a carbon source and the Rhodococcusopacus PD630 is a high-quality oil-producing strain. The invention provides a method for producing the fatty acid by efficiently utilizing corncob residues via the Rhodococcusopacus PD630. Compared with common saccharomycetes, the Rhodococcus has strong bearing capability on polar conditions and high carbon source conversion capability; and a fermentation condition is simple and the method is an industrial production manner of the biodiesel, which has very great potential.

Description

technical field [0001] The invention relates to a biodiesel production method, in particular to a method in which rhodococcus utilizes fatty acids produced from xylose residues, and then uses a biodiesel production process to convert the produced fatty acids into biodiesel. Background technique [0002] Since the 20th century, petroleum has been a source of energy exploited and used by humans on a large scale. In the late 20th century, human beings gradually realized the shortage of oil resources and the environmental problems caused by their use. Therefore, in order to solve the dual pressure of energy and environment, relevant researchers have also been committed to the development of new energy. Among them, biodiesel has low sulfur content, does not contain aromatic compounds, is biodegradable, and is an environmentally friendly renewable energy source. The raw materials for biodiesel production come from a wide range of sources. With the development of society and tech...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/01
CPCC12P7/649Y02E50/10Y02P20/59
Inventor 朱道辰谢长校陈焱
Owner ZHENJIANG BIO INNOVA BIOTECH
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