Application of Dickeya Zeae antibiotic zeamines in control of plant diseases

A technology for rice basal rot fungus and plant disease, which is applied in the directions of chemicals, applications, and biocides for biological control, can solve problems such as no reports, and achieve the effect of solving resistance problems.

Active Publication Date: 2015-02-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The application of zeamines in the control of plant diseases has not been reported, especially in the prevention and control of postharvest diseases of agricultural products.
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Method used

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  • Application of Dickeya Zeae antibiotic zeamines in control of plant diseases
  • Application of Dickeya Zeae antibiotic zeamines in control of plant diseases
  • Application of Dickeya Zeae antibiotic zeamines in control of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of zeamines

[0021] OD 600 =1.0 D. zeae The seed solution was inserted into the toxin-producing medium (LS medium), 25 o After C, 180rpm cultivated for 36 hours, the rice base rot pathogen was collected by centrifugation D. zeae Fermentation broth supernatant. The obtained fermentation broth was stored in a 2L Erlenmeyer flask sealed and protected from light until the next step of extraction.

[0022] The present invention adopts the conventional compound separation and extraction method to separate and extract the antibacterial active substances in the fermentation broth. The pH value of the fermentation broth was adjusted up to 10 with 5M NaOH, and then the supernatant of the fermentation broth with a pH of 10 was concentrated to 1 / 10 of the original volume using a rotary evaporator. Extract twice with three volumes of organic solvent (n-butanol: ethyl acetate = 2:1). After the organic solvent was evaporated to dryness with an evap...

Embodiment 2

[0023] Example 2 zeamines against Pythophthora sp. ( Peronophythora Chen) inhibitory effect on bacteria.

[0024] Use 10% methanol as a solvent to dissolve the extract, and then configure it with CDA (carrot medium) medium to form a medium with zeamines, and use the mycelial growth rate method to determine the inhibitory effect of different concentrations of extracts on the mycelial growth of Pythora lychee . The specific operation is:

[0025] Mycelia growth rate method: use 10% methanol as a solvent to configure zeamines into a certain concentration of the test agent, and accurately add a certain amount of zeamines solution to 50o In the melted CDA medium, the final concentrations of zeamines in the medium were 0.5 μg / mL, 1 μg / mL, 2 μg / mL, 4 μg / mL, and 8 μg / mL, and each concentration was poured into three replicate culture plates. The same volume of 10% methanol was used as a control. After being cultivated for 5 days on a medium plate with a diameter of 9 cm for the ...

Embodiment 3

[0026] Example 3 : zeamines against Pythophthora sp. ( Peronophythora Chen) inhibitory effect on bacteria.

[0027] Using glass slides to measure (Fang Zhongda, 1998), the sporangia of Peronospora lychee cultured on CDA medium for 6 days were washed with sterile water, the sporangia solution was prepared, and the concentration of sporangia was adjusted to each visual field (10 ×10 times) about 40~60 sporangia. Add different amounts of zeamines solutions to the sporangia suspension, so that the final concentrations are 0.5 μg / mL, 1 μg / mL, 2 μg / mL, 4 μg / mL, and 8 μg / mL. Take 50 μL of spore suspension and drop it on the concave glass slide, then place the concave glass slide in a 9cm petri dish with a piece of filter paper, and drop a small amount of sterile water on the filter paper to keep it moist, cover the petri dish, and place it at 28 o After culturing in the dark for 6 hours, the sporangia germination was observed under an optical microscope. Experimental results s...

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PUM

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Abstract

The invention discloses new application of Dickeya Zeae-produced antibiotic substance zeamines in control of plant diseases, and the new application is specifically used in control of fungal plant diseases. By indoor bioassay and pot experiments, the application finds that the Dickeya Zeae-produced antibiotic zeamines has good control effect on PeronophythoraChen, ColletotrichumCorda,Pyricularia, Botrytissp., Rhizoctonia, Fusarium and other common plant diseases. The invention discloses a new thought for new application of the zeamines in control of the plant diseases.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to the application of the rice base rot antibiotic zeamines in plant disease control, in particular to the application of the rice base rot antibiotic zeamines as a fungicide in plant diseases. Background technique [0002] The emergence of the science of biological control begins with the use of natural enemies to control harmful organisms. In the 1880s, the introduction of Australian ladybugs from Australia to California in the United States achieved great success in controlling the cotton scale, which caused a sensation in the field of international entomology, and a new era of traditional biological control science was born. After more than 100 years of development, biological control has become a subject with relatively complete basic theoretical system and methodology in theory and method. In 1987, the National Academy of Sciences of the United States expanded the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00
Inventor 张炼辉廖立胜陈少华贺飞周佳暖王惠杉
Owner SOUTH CHINA AGRI UNIV
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