A tissue engineering nanofiber osteochondral repair scaffold and its preparation method

Abstract

The invention discloses a nanofiber bone cartilage repairing stent for tissue engineering and a preparation method thereof. The nanofiber bone cartilage repairing stent for tissue engineering is characterized by comprising a porous cartilage layer, a porous cartilage lower bone layer and a connecting layer between the porous cartilage layer and the porous cartilage lower bone layer, wherein the porous cartilage layer includes a PLLA-based composite degradable polymer material and is of a porous nanofiber structure which is 20-400mu m in pore diameter; the connecting layer includes a PLLA-based composite degradable polymer material and is of a porous nanofiber structure which is less than 5mu m in pore diameter; and the porous cartilage lower bone layer comprises a PLLA-based composite degradable polymer material containing 5-70% (w / w) of nano-hydroxyapatite and is of a porous nanofiber structure which is 50-500mu m in pore diameter. Microstructure of the bone cartilage repairing stent prepared by the invention is represented as bionic ECM nanofiber; the repairing stent can promote adhesion, propagation and differentiation of related bone cartilage cells on the stent, and can also promote transportation of nutrient substances in the stent and discharge of metabolic waste.

Description

technical field [0001] The invention belongs to the technical field of osteochondral tissue engineering scaffolds, in particular to a tissue engineering nanofiber osteochondral repair scaffold and a preparation method thereof. Background technique [0002] The injury and disease of articular cartilage is a common orthopedic disease in clinical practice, and it is difficult to repair itself. Moreover, cartilage damage in joints is often accompanied by combined damage to the subchondral bone layer, so repairing the cartilage also requires repairing the subchondral bone. At present, the commonly used clinical treatment method is autologous or allogeneic osteochondral transplantation, which has achieved certain therapeutic effects, but there are also shortcomings. Risk of disease transmission. The development of tissue engineering provides a new way to construct scaffolds for osteochondral repair in vitro. [0003] Scaffold is an important part of tissue engineering, which pl...

AI Technical Summary

Problems solved by technology

Chinese patent (CN102872480A) discloses a biphasic osteochondral tissue engineering scaffold, its cartilage layer and subchondral bone layer are both porous structures, but microscopically it is not the nanofibrous structure of bionic ECM

Moreover, since the two layers are connected porous structures, it is easy for the related cells of the cartilage layer and the subchondral bone layer to migrate and grow into each other, which affects the repair of the functions of the cartilage layer and the subchondral bone layer.

Images