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Technology for extracting polymyxin B through precipitation method

A technology of polymyxin and precipitation method, which is applied in the field of medicine and can solve problems such as environmental pollution, low product content, and short process

Inactive Publication Date: 2015-02-04
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a process for extracting polymyxin B with short process, high content, and environmental friendliness, which solves the problem of long process for extracting polymyxin B in the prior art, serious environmental pollution, and the resulting product low content problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] a. Polymyxin B fermentation broth 200L, content 1.2mg / mL, add oxalic acid to adjust pH to 1.5, filter with ceramic membrane at room temperature, cool the filtrate to 18°C, add 5% sodium hydroxide to adjust pH value is 6.9, the gained solution is transferred to the D152 resin column for adsorption, the resin column is washed with purified water until the light transmittance of the effluent is 98%, then the sulfuric acid of 0.02mol / L is used to prewash the resin column, and finally the resin column is washed with 0.2mol / L The sulfuric acid of / L analyzes the resin column, and collects the analysis solution;

[0028] b. add 5% sodium hydroxide to the analysis solution obtained in step a to adjust the pH value to 9.0, then filter to obtain solid 410g (wet product), and the separated solid is washed with purified water 30L, to the solid after washing Add 1000mL of purified water to the solution, stir to disperse the solid in the purified water evenly, then add 0.03mol / L sulf...

Embodiment 2

[0031] a. Polymyxin B fermentation broth 250L, content 0.9mg / mL, add oxalic acid to adjust the pH value to 1.0, filter it with a plate frame at room temperature, cool the separated liquid to 17°C, add 5% sodium hydroxide The pH value of the solution is adjusted to 6.0, the gained solution is transferred to the D152 resin column for adsorption, the resin column is washed with purified water until the light transmittance of the effluent is 98.5%, then the resin column is pre-washed with 0.01mol / L sulfuric acid, and finally Analyze the resin column with 0.1mol / L sulfuric acid, and collect the analysis solution;

[0032] b. Add 5% sodium hydroxide to the analysis solution obtained in step a to adjust the pH value to 8.0, filter the precipitate to obtain 425 g of solid (wet product), wash the separated solid with 35 L of purified water, and add to the solid after washing Add 1200mL of purified water, stir to disperse the solid in the purified water evenly, then add 0.05mol / L sulfur...

Embodiment 3

[0035] a. Polymyxin B fermentation broth 150L, ​​content 1.6mg / mL, add oxalic acid to adjust the pH value to 2.0, filter with ceramic membrane at room temperature, separate solid and liquid, cool the separated liquid to 15°C, add 6% sodium hydroxide solution adjusts the pH value to 8.0, and the resulting solution is adsorbed with a D152 resin column, and the resin column is washed with purified water until the light transmittance of the effluent is 99%, and then the resin column is treated with 0.05mol / L sulfuric acid. Pre-wash, and finally analyze the resin column with 0.5mol / L sulfuric acid, and collect the analysis solution;

[0036] b. add 6% sodium hydroxide solution to the solution obtained in step a to adjust the pH value to 10.0, then filter to obtain solid 450g (wet product), the separated solid is washed with purified water 40L, to the washed Add 1500mL of purified water to the solid, stir to disperse the solid evenly in the purified water, then add 0.04mol / L sulfuri...

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Abstract

The invention discloses a technology for extracting polymyxin B through a precipitation method. In the technology, by means of the precipitation method, a polymyxin B fermentation liquid is subjected to precipitation with oxalic acid, wherein a precipitated and separated solid is dissolved with addition of an alkaline substance and then is adsorbed by a weak acidic cation exchange resin. The weak acidic cation exchange resin is washed by purified water and is per-washed and desorbed with dilute sulfuric acid. A desorption solution is subjected to precipitation with an alkaline substance and acidic substances are dissolved. Solid-liquid separation is carried out with a liquid being subjected to decoloration and nano-filtration and then being dried to obtain the polymyxin B. The technology is short in required processes and is free of organic solvents. The polymyxin B is high in content and is suitable for industrialized production and application.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a process for extracting polymyxin B by a precipitation method. Background technique [0002] Polymyxin is a basic cyclic polypeptide with good antibacterial activity synthesized and secreted by Bacillus polymyxa in the metabolic process. Polymyxin B is a kind of polymyxin, and its sulfate is commonly used. It is white Crystalline powder, easily soluble in water, has hygroscopicity, stable in acidic solution, the neutral solution will not affect the potency after being placed at room temperature for a week, and the alkaline solution is unstable, almost in ether, ketone, ester, hydrocarbon and other organic solvents insoluble. [0003] Polymyxin B has a strong antibacterial effect on most Gram-negative bacilli, and it has a more significant effect on Pseudomonas aeruginosa. Klebsiella pneumoniae, etc. also have a good effect. Polymyxin B is effective for bacteria i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/62C07K1/36C07K1/30C07K1/18
Inventor 宋志倩谷新华
Owner HEBEI SHENGXUE DACHENG PHARMA
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