A kind of production method of yellow water vinegar drink
A technology of vinegar drink and vinegar mother, which is applied in the production field of yellow water vinegar drink, can solve the problems of environmental pollution and the failure to maximize the additional benefits of yellow water, etc., achieve broad market prospects, low production cost, and sweet taste in the mouth luscious effect
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Embodiment 1
[0036] Embodiment 1 produces yellow water vinegar drink according to the following steps:
[0037] (1) Preparation of lactic acid bacteria liquid: prepare 700ml of MRS medium, adjust pH to 6.3, distribute into seven 300ml Erlenmeyer flasks, autoclave at 121°C for 30min, and cool to room temperature; Lactobacillus plantarum subsp. Lactobacillusplantarumsubsp.plantarum) CGMCCNO.1.2158 Under sterile conditions, pick 3 rings into each triangular flask with an inoculation loop and incubate at 31°C for 48 hours to obtain lactic acid bacteria seed liquid; add 7L medium to a 10L fermenter (The fermenter medium is composed of MRS medium and yellow water, MRS: yellow water volume ratio = 7:3) sterilized at 121°C and 0.15Mpa for 30min, cooled to room temperature, and 8% (v / v) under sterile conditions ) ratio into the triangular flask lactic acid bacteria seed liquid, 31 ℃, static culture for 42 hours, to obtain 7L lactic acid bacteria liquid.
[0038] (2) Preparation of wine mother: pre...
Embodiment 2
[0044] Embodiment 2 produces yellow water vinegar drink according to the following steps:
[0045] (1) Preparation of lactic acid bacteria liquid: prepare 700ml of MRS medium, adjust the pH to 6.4, divide into seven 300ml Erlenmeyer flasks, autoclave at 121°C for 30min, and cool to room temperature; Under sterile conditions, pick 4 loops and insert them with inoculation loops in each triangular flask, and cultivate them at 32°C for 48 hours to obtain lactic acid bacteria seed liquid; add 7L culture medium to a 10L fermenter (fermenter culture medium is cultivated by MRS base and yellow water, MRS: yellow water = 7:3 (v / v)) sterilized at 121°C and 0.15Mpa for 30 minutes, cooled to room temperature, and inserted at a ratio of 9% (v / v) under sterile conditions The lactic acid bacteria seed liquid in the triangular flask was cultured at 32° C. for 44 hours to obtain 7 L of lactic acid bacteria liquid.
[0046] (2) Preparation of wine mother: prepare 300ml corn mash medium, adjust...
Embodiment 3
[0052] Embodiment 3 produces yellow water vinegar drink according to the following steps:
[0053] (1) Preparation of lactic acid bacteria liquid: prepare 700ml of MRS medium, adjust the pH to 6.5, divide into seven 300ml Erlenmeyer flasks, autoclave at 121°C for 30min, and cool to room temperature; Under sterile conditions, pick 5 rings and insert them with inoculation loops in each triangular flask, and cultivate them at 33°C for 48 hours to obtain the lactic acid bacteria seed liquid; add 7L culture medium to a 10L fermenter (fermenter culture medium is cultivated by MRS base and yellow water (7:3 (v / v)); sterilized at 121°C and 0.15Mpa for 30 minutes, cooled to room temperature, and inserted into the Erlenmeyer flask with lactic acid bacteria seeds at a ratio of 10% (v / v) under sterile conditions Liquid, 33 ℃, static culture 45 hours, obtain 7L lactic acid bacteria liquid.
[0054] (2) Preparation of wine mother: prepare 300ml corn mash medium, adjust pH to 4.5, divide in...
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