Peptide vaccines for the prevention of foot-and-mouth disease

A construct and dendritic technology, applied in the field of preparing these constructs, can solve the problems of uncertain nature of infectious virus release, difficulty in distinguishing, etc.

Inactive Publication Date: 2015-02-18
POMPEU FABRA UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Despite advances in recent years, vaccines known and in use until now have shown several disadvantages, such as the need for a cold chain to maintain their stability, the need for periodic repeat vaccinations, the release of infectious virus during vaccine production risk and its uncertain nature, in other words, the difficulty of serologically distinguishing infected animals from those that have been vaccinated

Method used

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  • Peptide vaccines for the prevention of foot-and-mouth disease
  • Peptide vaccines for the prevention of foot-and-mouth disease
  • Peptide vaccines for the prevention of foot-and-mouth disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0096] General peptide synthesis scheme.

[0097] Linear peptides were synthesized on a Fmoc-Rink-amide ChemMatrix resin at a 0.1 mmol scale by the automated Fmoc synthesis (FastMoc) protocol performed in an ABI433 peptide synthesizer (Applied Biosystems, Foster City, CA). Its side chain functional group utilizes t Bu (aspartic acid, glutamic acid, serine, threonine, tyrosine), Boc (lysine, tryptophan), Pbf (arginine) and Trt (aspartyl, glutamine , histidine) group protection. An 8-fold excess of Fmoc-L-amino acid and HBTU was used for the coupling step in the presence of twice the molar amount of DIEA in DMF as solvent. Branching is introduced by incorporation of Fmoc-Lys(Fmoc)-OH at appropriate points. After derivatization, using TFA / H 2 O / TIS (95:2.5:2.5v / v, 90min, RT), the peptide was completely deprotected and cleaved, precipitated by addition of chilled ether, taken up in aqueous AcOH (10% v / v) and lyophilized. It was then purified and characterized by analytical HP...

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Abstract

The invention relates to a dendrimeric peptide construct of formula (I) wherein: T is a T epitope of the FMDV, bound to the lysine core by its N-terminal end, B is a B epitope of the FMDV, W is a linker group, Z is Cys bound to the linker group W through its sulphur atom or Lys bound to the linker group W through its µ amino group, X is absent when B is bound to the ±-carboxylic group of Z by its N-terminal end or is an acyl group when B is bound to the ±-amino group of Z by its C-terminal end. The invention also refers to the use of said constructs as an immunogen for the prevention of foot-and-mouth disease in an animal.

Description

technical field [0001] The present invention relates to a lysine core based dendrimeric peptide construct comprising both B and T epitopes of Foot and Mouth Disease Virus (FMDV). The invention also relates to methods for preparing these constructs, compositions comprising these constructs and their use in the prevention of foot-and-mouth disease in animals. Background technique [0002] Foot-and-mouth disease virus (FMDV) is a picornavirus that produces a highly contagious and devastating disease in artiodactyls. Because foot-and-mouth disease is highly contagious, it is a serious plague for the livestock industry. Its containment requires considerable efforts in vaccination, rigorous monitoring, trade restrictions and quarantine, and sometimes the culling of millions of animals. [0003] FMDV particles comprise a positive-strand RNA molecule of approximately 8500 nucleotides encapsulated within an icosahedral capsid containing 60 copies of each of the four viral proteins ...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K39/135
CPCA61K47/48253A61K39/12A61K2039/645C12N2770/32134A61K2039/55566A61K47/641A61K2039/552A61P31/14
Inventor D·安德勒马丁内斯J·巴尔塞纳德尔里勾E·布兰科拉维利亚C·库维略斯萨帕塔B·加西亚德拉托雷M·蒙索奥利瓦雷斯F·索布里偌卡斯特略
Owner POMPEU FABRA UNIVERSITY
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