ECM1 polypeptide epitopes bound to human MHC-class I molecules

A MHC-I, -1ECM1 technology, applied in the direction of medical preparations containing active ingredients, peptides, analytical materials, etc., to achieve the effect of large commercial industrialization, convenient clinical application, and improved accuracy
CN104163852BActive Publication Date: 2016-09-28魏敏杰
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
魏敏杰
Publication Date
2016-09-28

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Abstract

The ECM1 polypeptide epitope combined with human MHC-I class molecules is characterized in that: the ECM1 polypeptide epitope combined with human MHC-I class molecules can bind to the binding site of human MHC-I class molecules and can activate specific The polypeptide epitope of cytotoxic T lymphocytes is the following amino acid sequence selected from human extracellular matrix protein-1 (ECM1) and having homology with human ECM1. The ECM1 polypeptide epitope that binds to human MHC-I class molecules is selected from free-type polypeptides, fusion-type polypeptides and chimeric polypeptides in ECM18-16 and ECM1409-417; and one of the five polypeptides A polymer of various forms of monomers. The invention has the advantages of further improving the accuracy of predicting the polypeptide epitope, and simultaneously predicting the polypeptide epitope from the spatial conformation. It is easily synthesized in vitro and easily absorbed by the body after intravenous administration, so it has a very large commercial industrialization value.
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Description

technical field

[0001] The invention relates to the technical field of biomedical engineering, including screening and obtaining ECM1 polypeptide molecules combined with human major histocompatibility complex (MHC) class I molecules, as well as DNA and RNA encoding these polypeptides, because these polypeptides are compatible with human MHC-I Binding to the binding sites of the class of molecules can activate specific cytotoxic T lymphocytes (CTL cells), thereby effectively killing ECM1-positive tumor tissues and achieving the purpose of treating tumors. Background technique

[0002] Extracellular matrix protein-1 (ECM1) is a secreted glycoprotein isolated from the mouse osteogenic stromal cell line MN7 by Mathieu et al. in 1994. Its relative molecular mass is 85KD, and it has a hydrophobic signal peptide and several Glycosylation site. The gene is located on human chromosome 1q21 adjacent to the upper epidermal differentiation complex region, contains 11 exons, and is abou...

Claims

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