Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Uses of αb-crystallin

A crystallin and protein technology, applied in the field of biomedicine, can solve problems such as unclear mechanism of action

Inactive Publication Date: 2017-01-04
GENERAL HOSPITAL OF CHINESE PEOPLES ARMED POLICEFORCES
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although many studies have demonstrated that αB-crystallin has a protective effect on RGCs, its mechanism of action is still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Uses of αb-crystallin
  • Uses of αb-crystallin
  • Uses of αb-crystallin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Establishment of Rat Acute Ocular Hypertension Model

[0030]Methods: Rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (0.4ml / 100g). After successful general anesthesia, the conjunctival sac of the right eye was flushed with levofloxacin eye drops, and then one drop of compound tropicamide eye drops and one drop of oxybucaine hydrochloride eye drops were added, with an interval of 5 minutes between the two eye drops. Under a dissecting microscope, a 33G needle connected with a saline infusion set was inserted into the anterior chamber through a tunnel incision at the limbus, taking care not to injure the iris and lens, and the infusion regulator was opened to the maximum. Keep the liquid level at a vertical distance of 156cm from the mouse eye (generating 15.22kPa, equivalent to an intraocular pressure of 120mmHg). When there is no liquid instillation in Murphy's duct and the bulbar conjunctiva, iris, and retina are pale, the model o...

Embodiment 2

[0031] Example 2 Drug injection into the vitreous cavity of rat acute ocular hypertension model

[0032] Preparation of αB-crystallin solution: Dissolve commercial αB-crystallin dry powder (purchased from sigma) in normal saline to make the final concentration 1*10 -5 g / L.

[0033] Method: After the rat model of acute ocular hypertension was established, use a micro-injector to pierce the vitreous cavity behind the limbus of the right eye of the rat under a microscope. An enlarged needle can be seen from the center of the cornea. Slowly inject, pull out the needle, and the conjunctiva Apply erythromycin eye ointment intracapsularly. Among them, 5 μl of isotonic saline was injected into the vitreous cavity of the rats in group B, and 1*10 -5 g / L αB-crystallin solution 5 μl. Gently press the cornea with a cover glass to observe whether there is bleeding and leakage at the injection site of the vitreous cavity, whether the retinal reflection of the fundus is normal, and whethe...

Embodiment 3

[0034] Embodiment 3 QRT-PCR method detects the mRNA level of kv1.1, kv1.3, Bcl-xL and caspase-3

[0035] step:

[0036] 1. Extraction of retinal total RNA

[0037] At 7d and 14d after operation, 5 rats in each group were anesthetized intraperitoneally with 10% chloral hydrate. Take out the operated eye and rinse it in normal saline in an ice bath for 3min*2 times. Under the microscope, remove the anterior segment and remove the retinal tissue. Total RNA in retinal tissue was extracted according to the instructions of the Ultrapure RNA Extraction Kit (purchased from Beijing Kangwei Century Biotechnology Co., Ltd.).

[0038] 2. RNA quantification

[0039] Take 2 μl of RNA, use air as a blank control, and use an ABI nucleic acid quantitative analyzer to detect the purity of RNA. The OD260 / 280 ratio of pure RNA is close to 2.0, and the OD260 / 280 ratio of DNA is close to 1.8. The OD260 value was measured with an ABI accounting quantitative analyzer for accurate quantification....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the use of αB-crystallin in the preparation of drugs for inhibiting potassium ion channels in retinal ganglion cells. The present invention injects αB-crystallin into the rat model of acute ocular hypertension, and compared with the non-injected group, the expressions of kv1.1 and kv1.3 on the retina of the rats in the injection group decrease, indicating that αB-crystallin has the function of inhibiting retinal nerve Function of potassium channels in ganglion cells. The invention provides a new drug candidate for clinically treating related diseases by inhibiting the function of potassium ion channels in retinal ganglion cells.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a new application of αB-crystallin, in particular to the application of αB-crystallin in the preparation of drugs for inhibiting potassium ion channels in retinal ganglion cells. Background technique [0002] Retinal ganglion cells (RGCs) are the last stop of information transmission in the retina, and their axons first form the nerve fiber layer of the retina, and then converge into the optic nerve, playing an important role in the conduction of the visual pathway. Optic nerve damage can cause optic neuropathy, resulting in visual loss, which can be seen in many ophthalmological diseases, such as optic neuritis, visual path space-occupying lesions, glaucoma, ischemic optic neuropathy, and diabetic retinopathy. In recent years, the research on optic nerve protection has attracted the attention of many scholars at home and abroad. Studies have shown that the main pathological ba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61P9/10
Inventor 吴志鸿侯世科樊毫军马艳
Owner GENERAL HOSPITAL OF CHINESE PEOPLES ARMED POLICEFORCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com