Method for improving chilling resistance of plant via glycine betaine

A betaine and cold resistance technology, which is applied in the field of pesticides, can solve the problems that the safety of genetically modified products is not fully proved, and it is not suitable to be used in crop production, and achieves the effects of wide application range, improved resistance, and improved cold resistance.

Inactive Publication Date: 2015-03-11
朱小丽
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The safety of genetically modified products produced by this technology has not been fully proved and should not be used in crop production

Method used

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  • Method for improving chilling resistance of plant via glycine betaine
  • Method for improving chilling resistance of plant via glycine betaine
  • Method for improving chilling resistance of plant via glycine betaine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Seed soaking in betaine aqueous solution improves germination ability of corn seeds under low temperature stress

[0022] Treatment ①: Before accelerating germination, mix boiling water and cold water in half, take a certain amount of pure betaine to prepare an aqueous solution, and use 2 to 3 kg of betaine in 1000 liters, and put 60 selected corn seeds into the betaine aqueous solution During the process, the liquid level should exceed the seeds by 20-25 cm, and at the same time, stir with a wooden stick in time to make the seeds evenly heated. The water temperature should be controlled at 25-28 degrees Celsius. Soak the seeds for 3-5 hours under this temperature condition. After the seeds absorb water and swell, remove the seeds and divide them into two groups on average; treatment ② is the control: before germination, divide the boiled water and cold water in half Right, put the selected 60 corn seeds into warm water, the water surface should be 20-25 c...

Embodiment 2

[0026] Example 2: Foliar spraying of betaine under low temperature stress improves wheat viability

[0027] The betaine solution with a concentration of 30kg / 1000L is configured, and 40 wheat plants with the same growth and no damage by diseases and insect pests are selected and divided into two groups for treatment ①: foliar spraying of betaine, treatment ② control: foliar spraying of clear water, foliar spraying Spray the amount to cover the entire leaf surface until there are small drops of water dripping, air-dry and absorb naturally, after the surface of all wheat leaves has no moisture, put them in a ‐2°C constant temperature box for 12 hours of low temperature stress, and use an EGM-4 photosynthetic instrument to measure the total photosynthetic content. amount to calculate leaf mortality. The test results are shown in Table 3 below:

[0028] Table 3 Total photosynthetic amount and leaf mortality of wheat under low temperature stress

[0029]

[0030] The test resu...

Embodiment 3

[0031] Example 3: Applying betaine to cucumber seedlings under low temperature stress improves the ability of cucumber to resist low temperature

[0032] Select 40 healthy cucumber seedlings with the same growth, and divide them into two groups on average for treatment ①: use the prepared betaine solution with a concentration of 0.5kg / 1000L to root-apply cucumber seedlings for 24 hours, and ② treat with clean water as a control. The two groups of materials were subjected to low temperature stress at 6°C for 2 days, and then the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were measured. The content of MDA was positively correlated with the degree of damage to the cell membrane. The lower the content of MDA, the more resistant the plant to cold. The better the resistance, the positive correlation between SOD activity and plant life activity, the higher the SOD activity, the better the cold resistance of plants. The test results are shown in Ta...

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Abstract

The invention discloses a method for improving chilling resistance of plant via glycine betaine, and belongs to the technical field of pesticides. The method for improving the chilling resistance of the plant via the glycine betaine has the advantages that four methods of soaking the seeds with glycine betaine, applying soil to root, spraying to leaf surfaces or soaking the fruit are conducted to process the plant of different types, and thus the chilling resistance of the plant and fruit can be obviously improved; the applicable scope is wide, and no potential risk is caused.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to a method for improving plant cold resistance by using betaine. Background technique [0002] Temperature is the main limiting factor determining the distribution of plants, and it is also the main factor affecting the yield and quality of crops and horticultural crops under cultivation conditions. Chilling injury refers to the damage to plants caused by low temperatures above zero. Chilling damage is generally 0-10°C. Chilling injury causes a series of physiological metabolic changes in plants, which eventually lead to weak growth of seedlings, slow growth of plants, wilting and yellowing, local necrosis, low fruit setting rate, reduced yield and quality. [0003] Wheat is an important food crop in my country, mainly distributed in the north of 30°N latitude. According to the growth habit, it can be divided into winter wheat and spring wheat. Compared with spring wheat, win...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01C1/00A01N37/44A01P21/00A23B7/154
CPCA01C1/00A01C21/005A01G22/00A01N37/44A23B7/154
Inventor 李润霞
Owner 朱小丽
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