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A method for extracting and utilizing pig blood

A pig blood and hemoglobin technology, which is applied in the field of pig blood processing, can solve the problems such as the decolorization effect needs to be improved and the ash content is large, and achieve the effects of improving the immunity of suckling pigs, low ash content and improving thermal stability.

Active Publication Date: 2017-06-09
浙江索纳克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ash content of the product prepared by this method is relatively large, and the decolorization effect still needs to be improved.

Method used

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  • A method for extracting and utilizing pig blood
  • A method for extracting and utilizing pig blood
  • A method for extracting and utilizing pig blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Preparation of porcine hemoglobin solution: After collecting the blood from qualified pigs, add disodium edetate, sodium bicarbonate and tea polyphenols or apple polyphenols in a mass ratio of 1:5:0.03. The weight ratio of disodium acetate to pig blood is 2:1000. After mixing well, centrifuge it for the first time at 25°C for 6 minutes to obtain the first supernatant layer and the first sedimentation layer. The first sediment is obtained after separation. Add deionized water to the sediment, and obtain a solution of porcine hemoglobin by ultrasonic treatment at 2500W ultrasonic power for 60 seconds;

[0060] Pre-treatment: Use nitrogen bubbling deoxygenation to carry out the first physical deoxygenation and preliminary decolorization of the porcine hemoglobin solution, then adjust the pH of the deoxygenated porcine hemoglobin solution to 7, and then perform the second ultrasonic treatment under 800W ultrasonic power Get pre-treatment liquid in 90s;

[0061] Segmented enzyma...

Embodiment 2

[0071] Preparation of porcine hemoglobin solution: After collecting the blood of qualified pigs, add disodium edetate, sodium bicarbonate and tea polyphenols or apple polyphenols in a mass ratio of 1:2:0.01. The weight ratio of disodium acetate to pig blood is 1:1000. After mixing well, centrifuge for the first time at 15°C for 3 minutes to obtain the first supernatant layer and the first sedimentation layer. The first sediment is obtained after separation. Add deionized water to the sediment, and obtain a solution of porcine hemoglobin by ultrasonic treatment for 30 seconds under the ultrasonic power of 1800W;

[0072] Pre-treatment: Use nitrogen bubbling deoxygenation to perform the first physical deoxygenation and preliminary decolorization of the porcine blood hemoglobin solution, then adjust the pH value of the deoxygenated porcine hemoglobin solution to 6, and then perform the second ultrasonic treatment under the ultrasonic power of 600W Get the pre-treatment liquid in 50s...

Embodiment 3

[0080] The preparation of the porcine hemoglobin solution is specifically to collect the blood of qualified pigs, and add disodium edetate, sodium bicarbonate and plant phenols extracted from natural plants in a mass ratio of 1:3:0.03. The weight ratio of disodium aminetetraacetic acid to pig blood is 1.5:1000. After mixing, the first time disc centrifugal separation at 20°C for 4 minutes, the first supernatant layer and the first precipitation layer are obtained, and the first precipitate is obtained by separation. Add 1.8-2 times the volume of deionized water to the first precipitate, and obtain a porcine hemoglobin solution for the first 50s of ultrasonic treatment under the ultrasonic power of 2000W.

[0081] Pretreatment: Use nitrogen bubbling deoxygenation to perform the first physical deoxygenation and preliminary decolorization of the porcine blood hemoglobin solution, then adjust the pH value of the deoxygenated porcine hemoglobin solution to 6.5, and then perform the sec...

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Abstract

The present invention relates to a pig blood treatment method, particularly to a pig blood extracting and using method, which comprises: preparing a pig blood hemoglobin solution, wherein an additive is added to pig blood, centrifuging separation is performed to obtain the first supernatant layer and the first precipitation layer, separating is performed to obtain the first precipitate, and a first ultrasonic wave treatment is performed; pre-treating, wherein physical deoxidation is performed, the pH value is adjusted, and a second ultrasonic wave treatment is performed; carrying out sectional enzymolysis decolorization; carrying out active carbon decoloration, wherein active carbon with the weight of 1-2% of the mass of the solution is added, and a decoloration treatment is performed for 5-10 min at a temperature of 25-35 DEG C; post-treating, wherein the pH value is adjusted, and centrifuging separation is performed to obtain the second supernatant layer and the second precipitation layer; taking the second supernatant layer, and carrying out ultra-filtration concentration separation to obtain the second concentrated solution; preparing pig blood plasma protein powder, wherein the first supernatant layer is subjected to spray-drying; preparing pig hemoglobin powder, wherein the second concentrated solution is subjected to spray-drying; and preparing a heme peptide, wherein the second precipitation layer substance is taken and is subjected to roller drying. The method of the present invention has the good decolorization effect, and the product with the high protein content and the low ash content can be prepared.

Description

Technical field [0001] The invention relates to a method for processing pig blood, in particular to a method for extraction and utilization of pig blood. Background technique [0002] The protein content of red blood cells in pig blood is as high as 38%, accounting for more than 75% of the total protein in whole blood, of which more than 92% is hemoglobin. Therefore, pig blood cells are an excellent source of animal protein. Make blood cell powder for sale. However, because the blood cell powder contains heme, the blood cell powder is black-red and has a heavy bloody smell, which is unacceptable for bad senses, and its economic added value is not high. [0003] Heme is combined with four globins in the form of porphyrin iron to form hemoglobin, which is present in red blood cells. In the food industry, heme can replace the coloring agent nitrate and synthetic pigments in meat products, and the use of heme can reduce the carcinogenic effects of nitrite. In the pharmaceutical indu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/30A23K10/24
Inventor 刘观洲邓自伟沙欢李宁丰
Owner 浙江索纳克生物科技有限公司