A digestive lactobacillus with patulin-scavenging effect and application thereof
A technology for digesting Lactobacillus and patulin, applied in the field of microorganisms, can solve the problems of limited removal of patulin, low efficiency of patulin, little application of food and feed, etc., and achieves strong removal, good tolerance, and wide application. Foreground effect
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Embodiment 1
[0028] The preparation of embodiment 1 patulin standard substance mother liquor
[0029] The patulin is a standard product of patulin, purchased from Beijing Taileqi Technology Co., Ltd., and prepared into a 100mg / L mother solution during use. The preparation method is as follows: Accurately weigh 5 mg of patulin standard substance, dissolve it in 50 mL of ethyl acetate, mix well and dissolve, filter and sterilize with a 0.22 μm microporous membrane, and store at -20°C.
Embodiment 2
[0030] Example 2 Preparation of the biological preparation of Lactobacillus pepticum CGMCC No.9663 for removing patulin
[0031] Inoculate Lactobacillus digesta CGMCC No.9663 in the MRS medium with 2% inoculation amount, culture it in a shaker with a temperature of 30-37°C and a shaker speed of 150r / min-200r / min for 18-24h, then pass through 5000r / min, centrifuged for 10 min to obtain digested Lactobacillus cells, washed the centrifuged cells with 0.85% normal saline three times, and then centrifuged to obtain digested Lactobacillus CGMCC No.9663 biological preparation.
[0032] Alternatively, the cells were resuspended with a protective agent to reach a concentration of 10 10 CFU / mL. The protective agent is 100g / L skimmed milk powder, 100g / L trehalose and 150g / L sucrose. Next, the suspension was pre-cultivated for 60 minutes at a temperature of 37° C., then pre-frozen at -20° C. for 2-4 hours, and finally freeze-dried to obtain a biological preparation of digested Lactobac...
Embodiment 3
[0033] The effect of embodiment 3 patulin on the growth of Lactobacillus cGMCC No.9663
[0034] 1. Preparation of MRS culture medium containing different concentrations of patulin
[0035] According to needs, accurately measure a certain volume of the patulin standard product mother liquor whose concentration is 100 mg / L in Example 1, dry it with nitrogen, dissolve it in the MRS culture medium, and set the volume to 100 mL. For example, when preparing a patulin culture solution with a concentration of 1000 μg / L, first accurately measure 1 mL of a patulin standard substance with a concentration of 100 mg / L, blow it dry with nitrogen, dissolve it in the MRS culture solution, and set the volume to 100 mL to obtain the concentration 1000μg / L patulin culture solution.
[0036] 2. Analysis of the effect of patulin on the growth of Lactobacillus digesta CGMCC No.9663
[0037] Inoculate the activated digestive lactobacillus CGMCC No.9663 into the patulin MRS culture solution prepare...
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