Human gastric cancer cell line with cisplatin resistance and establishing method and application thereof

A gastric cancer cell line and a method for establishing a technology, applied in the biological field, can solve the problems of low biological diversity of human gastric cancer cell lines and large differences in the biological properties of gastric cancer, and achieve high scientific research value and development significance, high homogeneity and uniformity. good effect

Active Publication Date: 2015-03-11
SHANGHAI CHEMPARTNER CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a new cisplatin-resistant human gastric cancer cell line aiming at the existing human gastric

Method used

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  • Human gastric cancer cell line with cisplatin resistance and establishing method and application thereof
  • Human gastric cancer cell line with cisplatin resistance and establishing method and application thereof
  • Human gastric cancer cell line with cisplatin resistance and establishing method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of embodiment 1GAXC023 cell

[0048] A fresh resection specimen of clinical gastric cancer was obtained from Nantong Cancer Hospital (patient gender: female, age: 73 years old, ethnicity: Han, native place: Nantong City, Jiangsu Province.), and the clinical diagnosis was poorly differentiated gastric adenocarcinoma.

[0049] Rinse with fresh HBSS buffer (containing 500 U / ml penicillin G, 500 μg / ml streptomycin sulfate and 1.25 μg / ml amphotericin B), cut into small pieces of 20-50 mg, subcutaneously puncture and inoculate in immunodeficient animals SCID mice (SCID mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.); 70 to 90 days after puncture inoculation, the tumor-bearing animals were sacrificed, and the tumor tissues were taken out for primary culture and subculture of cancer cells.

[0050] The primary culture steps are as follows: cut the tumor tissue into small pieces, place them in a culture bottle, and incubat...

Embodiment 2

[0053] Biological characteristics and application of embodiment 2GAXC023 cells

[0054] In the present invention, RPMI1640 is used to cultivate and the GAXC023 cells are purified by a differential attachment method, so that they can grow and pass stably in vitro for a long time. When the cells are passed to more than 20 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out until the 50th generation has the same stable properties. Through experimental observation and verification, GAXC023 grown in vitro has a typical epithelial-like morphology, the main body is paving stone-like, and grows in a covering layer. When the density is high, it can show adhesive aggregation. Genetic studies have confirmed that the cells are aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors. The cells can form tumors in nude mice a...

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Abstract

The invention discloses a human gastric cancer cell line with cisplatin resistance and an establishing method and application thereof. The human gastric cancer cell line is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO:C2013152. The human gastric cancer cell line has the advantages of stable characters, stable multiple passages, high tumor formation rate, short incubation period and good uniformity, and provides a novel experimental material closer to the biological characteristics of clinical tumor for the study of gastric carcinoma. The human gastric cancer cell line has tumorigenicity, and the generated tumor has cisplatin resistance; compared with the passage parental tumor, the generated tumor can be used to analyze the in vitro and in vivo drug sensitivity and resistance correlation, and then establish in vitro and in vivo two related drug screening platforms; and the is cell line is ideal for basic research of gastric cancer and clinical early stage application.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a cisplatin-resistant human gastric cancer cell line and its establishment method and application. Background technique [0002] Gastric cancer is one of the most common cancers in the world and the second leading cause of cancer death in the world, second only to lung cancer. At the same time, gastric cancer is one of the most common malignant tumors in Asia, and has been paid attention to in East Asian countries such as China and Japan because of its high mortality rate. According to the data listed by the Clinical Oncology Collaborative Center (CSCO) of the Chinese Anti-Cancer Association, the incidence of gastric cancer in China has accounted for 42% of the world. About 300,000 people die from gastric cancer every year in my country, accounting for 23.2% of all malignant tumor deaths, and it has become the first cause of malignant tumor deaths. Therefore, new therapeutic strate...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02A01K67/027C12R1/91
Inventor 强福林汤旭蓁胡刚张一心杨雪艳方后顺谢付波秦宵然
Owner SHANGHAI CHEMPARTNER CO LTD
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