Method for preparing mannan-oligosaccharide through enzymolysis of fine konjak powder

A technology of oligomeric mannose and konjac fine powder, which is applied in the field of bioengineering, can solve the problems of high substrate viscosity, high concentration production, high cost of enzyme production and use, and low catalytic efficiency of key enzymes, and achieve production and use The effect of low cost, short production cycle, and simple separation steps

Inactive Publication Date: 2015-03-11
JIANGNAN UNIV
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  • Application Information

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Problems solved by technology

At present, mannose oligosaccharides are usually prepared by high-temperature hydrolysis, acid hydrolysis or alkaline hydrolysis. Although enzymatic hydrolysis has also been reported, the conditions are not mature enough.
The enzymatic production of mannose oligosaccharides is mainly limited by the low catalytic efficiency of the key enzymes used in the production, the high cost of enzyme production and use, and the high viscosity of the substrate, which makes it difficult to achieve high-concentration production, etc.

Method used

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  • Method for preparing mannan-oligosaccharide through enzymolysis of fine konjak powder

Examples

Experimental program
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Effect test

Embodiment 3

[0023] Embodiment 3 ultrafiltration method prepares konjac fine powder mannose oligosaccharide

[0024] Carry out the enzymolysis reaction with optimized reaction conditions. After the reaction, inactivate the enzyme in boiling water and cool it down. The enzymolysis solution is centrifuged through a 0.45μm water film and then transferred to an ultrafiltration device. The molecular weight cut-off is 2000kDa under a pressure of 0.2MPa. The ultrafiltration membrane is used for ultrafiltration, and the oligosaccharides with a molecular weight cutoff of <2000 are collected, and the ultrafiltration permeate is collected. The sugar solution was concentrated by a rotary evaporator and then freeze-dried, and samples were taken for high-performance liquid chromatography (HPLC) to detect the composition of the enzymatic hydrolysis product.

[0025] Embodiment 4 konjaku powder hydrolyzate component analysis

[0026] Weigh oligomannose freeze-dried powder and dissolve to a concentration ...

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Abstract

The invention provides a method for preparing mannan-oligosaccharide from fine konjak powder through the combination of an enzymic method and an ultra-filtration process. The mannan-oligosaccharide is obtained by performing enzymolysis on the fine konjak powder by utilizing recombination beta-mannase (reAuMan26A). A key enzyme used in the method has higher catalytic activity; the production and use cost of the enzyme is lower; the production period is short; pollution is avoided; purifying and separating steps are simple and convenient; the additional value of the fine konjak powder is greatly increased; a theoretical basis is laid for further refining the mannan-oligosaccharide; the greater industrial production and application potential, and a greater economic value are achieved.

Description

technical field [0001] The invention relates to a method for preparing mannose oligosaccharides by hydrolyzing konjac powder with biological enzymes, and belongs to the technical field of bioengineering. Background technique [0002] Mannooligosaccharides (Mannooligosaccharides, MOS) are oligosaccharides formed by 2 to 10 monosaccharide molecules linked by β-1,4 or β-1,3 glycosidic bonds. It is produced by hydrolyzing mannan (extracted from plants such as konjac, carob gum and guar gum) with β-mannanase produced by bacteria such as Bacillus and Bacillus licheniformis. Studies have shown that mannose oligosaccharides can effectively promote the specific proliferation of beneficial intestinal flora such as Bifidobacterium and Lactobacillus, reduce intestinal pH to inhibit the growth of harmful bacteria; Non-specific immunity, promoting the secretion of mannose-binding protein in the liver to enhance the body's immunity; it also has the characteristics of improving serum lipid...

Claims

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Application Information

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IPC IPC(8): C12P19/14C07H3/06C07H3/04C07H1/06C08B37/00
Inventor 李剑芳赵梅唐诗涵邬敏辰王春娟董运海
Owner JIANGNAN UNIV
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