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A kind of immunoglobulin igm immunoturbidimetric detection kit

A technology for immunoglobulin and detection kits, which is applied in the field of immunoglobulin IgM immunoturbidimetric detection kits, can solve problems such as low accuracy and sensitivity, limited popularization and application, and poor stability of detection reagents, and achieves Effects of enhanced stability and improved analytical sensitivity

Active Publication Date: 2016-03-02
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ordinary immunoglobulin IgM immunoturbidimetric assay reagents are not stable, and the accuracy and sensitivity are not high, which limits its clinical application.

Method used

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  • A kind of immunoglobulin igm immunoturbidimetric detection kit
  • A kind of immunoglobulin igm immunoturbidimetric detection kit
  • A kind of immunoglobulin igm immunoturbidimetric detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A conventional detection kit for immunoglobulin IgM immunoturbidimetric method, which comprises reagent R1, reagent R2 and calibrator.

[0024] Wherein the reagent R1 consists of:

[0025] pH7.6Tris buffer 18.16mmol / L

[0026] Sodium chloride 123.20mmol / L

[0027] Polyethylene glycol 4006%

[0028] Reagent R2 consists of:

[0029] Tris buffer pH7.618.16mmol / L

[0030] Goat anti-human IgM antibody 30ml / L

[0031] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0032] Add 2 μl of physiological saline, sample or calibrator, then add 250 μl of R1 reagent for pre-incubation for 5 minutes and read the absorbance A1, then add 50 μl of reagent R2 and react for 5 minutes, read the absorbance A2, and calculate ΔA.

[0033] The calibrator used in this example is the IgM calibrator produced by Jiufeng Runda Biotechnology Co., Ltd.

Embodiment 2

[0035] An immunoglobulin IgM immune turbidimetric detection kit, which comprises reagent R1, reagent R2 and calibrator.

[0036] Wherein the reagent R1 consists of:

[0037] pH7.6Tris buffer 18.16mmol / L

[0038] Sodium chloride 123.20mmol / L

[0039] Polyethylene glycol 4006%

[0040] Silica coated magnetic nanoparticles 0.1%

[0041] Reagent R2 consists of:

[0042] Tris buffer pH7.618.16mmol / L

[0043] Goat anti-human IgM antibody 30ml / L

[0044] Bovine serum albumin 20g / L

[0045] Kathon-CG0.05%

[0046] Concrete determination method is with embodiment 1.

Embodiment 3

[0048] An immunoglobulin IgM immune turbidimetric detection kit, which comprises reagent R1, reagent R2 and calibrator.

[0049] Wherein the reagent R1 consists of:

[0050] pH7.6Tris buffer 18.16mmol / L

[0051] Sodium chloride 123.20mmol / L

[0052] Polyethylene glycol 4006%

[0053] Silica coated magnetic nanoparticles 1%

[0054] Reagent R2 consists of:

[0055] Tris buffer pH7.618.16mmol / L

[0056] Goat anti-human IgM antibody 30ml / L

[0057] Bovine serum albumin 30g / L

[0058] Kathon-CG0.05%

[0059] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses an immunoglobulin IgM immunoturbidimetric method detection kit, which belongs to the technical field of clinical in vitro detection reagents. The kit of the present invention includes reagent R1, reagent R2 and calibrator. By adding a certain amount of silica-coated magnetic nanoparticles to reagent R1 and adding a certain amount of bovine serum albumin and Kathon-CG to reagent R2, the stability and analytical sensitivity of the kit were effectively improved. The linear range is also good, and the accuracy of the reagent is high, which is conducive to further popularization and use in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an immunoglobulin IgM immunoturbidimetric detection kit. Background technique [0002] Immunoglobulins are composed of heavy chains and light chains. According to the different chemical structures of the heavy chain (H chain) constant region, the corresponding immunoglobulins are divided into various types, namely immunoglobulin M (IgM), immunoglobulin M (IgM), immunoglobulin Globulin D (IgD), Immunoglobulin A (IgA), Immunoglobulin E (IgE), and Immunoglobulin G (IgG). [0003] Human immunoglobulin M (IgM) exists in human serum, accounting for 5-10% of the total serum immunoglobulins, with a serum concentration of about 1 mg / ml. It is mainly synthesized by plasma cells in the spleen. One IgM molecule is composed of 5 single The body is connected into a pentamer through the J chain, which has a high antigen binding value and is also the largest molecul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/543G01N33/6854
Inventor 甘宜梧董雯谭柏清谢清华王绮李静包兴艳
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD