Cerebrolysin hydrolysate injection and preparation method thereof
A technology of cerebroprotein hydrolyzate and injection, which is applied in the field of cerebroprotein hydrolyzate injection and its preparation, can solve problems such as anaphylactic shock, and achieve the effect of reducing the degree of allergic reaction
Inactive Publication Date: 2015-03-18
CHENGDU LIST PHARMA
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The invention discloses a cerebrolysin hydrolysate injection and a preparation method thereof. The preparation method of the cerebrolysin hydrolysate injection comprises the following steps: (1) preparing a fresh and healthy pig brain, removing adipose tissues, homogenizing, vacuumizing at 8Pa, maintaining the pressure for 10 minutes, introducing CO2 until the pressure is 15Pa, and maintaining the pressure for 20 minutes; (2) slowly adding the defatted brains obtained in the step (1) into liquid CO2 in an amount which is 7 times of that of the defatted brains, stirring for 50 minutes, cooling down to the temperate being from -70 to -80 DEG C, keeping for 3 hours, and then increasing the temperature to 45 DEG C within 8 minutes to gasify CO2; (3) adding water for injection in an amount which is 3 times of that of the frozen-thawed brains obtained in the step (2), uniformly stirring, further adding papain or pepsin of which the weigh is equivalent to 0.7% of the weight of the frozen brains, and carrying out hydrolysis at 37 DEG C for 3.5 hours to obtain an enzymatic hydrolysate; and (4) centrifuging the enzymatic hydrolysate, taking a supernatant liquid, adding 1%-10% of HS-15, carrying out activated carbon adsorption at the room temperature, carrying out decarbonization and filtering, regulating the pH value, and filtering after an intermediate is examined to be qualified, so as to obtain the cerebrolysin hydrolysate injection. By virtue of a brand new prescription and process, the prescription safety of the cerebrolysin hydrolysate injection is obviously improved.
Nervous disorderHydrolysed protein ingredients +2
Activated carbonProteolysis +9
- Experimental program(3)
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