Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cerebrolysin hydrolysate injection and preparation method thereof

A technology of cerebroprotein hydrolyzate and injection, which is applied in the field of cerebroprotein hydrolyzate injection and its preparation, can solve problems such as anaphylactic shock, and achieve the effect of reducing the degree of allergic reaction

Inactive Publication Date: 2015-03-18
CHENGDU LIST PHARMA
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] has been widely used in the treatment of dementia, cerebrovascular disease and ischemic encephalopathy since it was launched in the 1990s. It is an economical, safe, small adverse reaction, It is a highly effective brain cell energizing agent, but cerebroprotein hydrolyzate injection can also cause anaphylactic shock. Allergic reactions such as abdominal pain, and anaphylactic shock in severe cases
Now check a number of commercially available cerebroprotein hydrolyzate injections and find that the instructions contain Tween-80. Modern medical literature has confirmed that Tween-80 is the main cause of adverse reactions (anaphylaxis) during clinical use.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 Preparation method of cerebroprotein hydrolyzate injection: ①Take healthy fresh pig brain, remove fat, homogenate, evacuate to 8Pa, keep the pressure for 10min, and then inject CO 2 To a pressure of 15Pa, keep the pressure for 20min; ②At 400rpm, slowly add the brain plasma in ① to 7 times its liquid CO 2 After the addition, stir for 50 minutes, cool down to -75°C, keep it for 3 hours, then raise the temperature to 45°C within 8 minutes, put CO 2 Gasification; ③ Add 3 times the weight of water for injection to step ②, stir evenly, then add papain or pepsin equivalent to 0.7% of the weight of frozen brain pulp, and hydrolyze at 37°C for 3.5 hours to obtain an enzymatic hydrolyzate; ④ Centrifuge the enzymatic hydrolyzate at 2500rpm for 25min, take the supernatant, take 100ml of the supernatant and the total nitrogen content is 6g (refer to the WS1-X-018-2001Z quality standard to detect the total nitrogen content), add 10ml of HS-15, in Activated carbon adsorpt...

Embodiment 2

[0012] Example 2 Preparation method of cerebroprotein hydrolyzate injection: ①Take healthy fresh pig brain, remove fat, homogenate, evacuate to 8Pa, keep the pressure for 10min, and then inject CO 2 To a pressure of 15Pa, keep the pressure for 20min; ②At 400rpm, slowly add the brain plasma in ① to 7 times its liquid CO 2 After the addition, stir for 50 minutes, cool down to -75°C, keep it for 3 hours, then raise the temperature to 45°C within 8 minutes, put CO 2 Gasification; ③ Add 3 times the weight of water for injection to step ②, stir evenly, then add papain or pepsin equivalent to 0.7% of the weight of frozen brain pulp, and hydrolyze at 37°C for 3.5 hours to obtain an enzymatic hydrolyzate; ④ Centrifuge the hydrolyzate at 2500rpm for 25min, take the supernatant, take 100ml of the supernatant and the total nitrogen content is 6g (refer to the WS1-X-018-2001Z quality standard to detect the total nitrogen content), add 50ml of HS-15, in Activated carbon adsorption at roo...

Embodiment 3

[0013] Example 3 Preparation method of cerebroprotein hydrolyzate injection: ①Take healthy fresh pig brain, remove fat, homogenate, evacuate to 8Pa, keep the pressure for 10min, and then inject CO 2 To a pressure of 15Pa, keep the pressure for 20min; ②At 400rpm, slowly add the brain plasma in ① to 7 times its liquid CO 2 After the addition, stir for 50 minutes, cool down to -75°C, keep it for 3 hours, then raise the temperature to 45°C within 8 minutes, put CO 2 Gasification; ③ Add 3 times the weight of water for injection to step ②, stir evenly, then add papain or pepsin equivalent to 0.7% of the weight of frozen brain pulp, and hydrolyze at 37°C for 3.5 hours to obtain an enzymatic hydrolyzate; ④ Centrifuge the hydrolyzate at 2500rpm for 25min, take the supernatant, take 100ml of the supernatant and the total nitrogen content is 6g (refer to the WS1-X-018-2001Z quality standard to detect the total nitrogen content), add 100ml of HS-15, in Activated carbon adsorption at r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cerebrolysin hydrolysate injection and a preparation method thereof. The preparation method of the cerebrolysin hydrolysate injection comprises the following steps: (1) preparing a fresh and healthy pig brain, removing adipose tissues, homogenizing, vacuumizing at 8Pa, maintaining the pressure for 10 minutes, introducing CO2 until the pressure is 15Pa, and maintaining the pressure for 20 minutes; (2) slowly adding the defatted brains obtained in the step (1) into liquid CO2 in an amount which is 7 times of that of the defatted brains, stirring for 50 minutes, cooling down to the temperate being from -70 to -80 DEG C, keeping for 3 hours, and then increasing the temperature to 45 DEG C within 8 minutes to gasify CO2; (3) adding water for injection in an amount which is 3 times of that of the frozen-thawed brains obtained in the step (2), uniformly stirring, further adding papain or pepsin of which the weigh is equivalent to 0.7% of the weight of the frozen brains, and carrying out hydrolysis at 37 DEG C for 3.5 hours to obtain an enzymatic hydrolysate; and (4) centrifuging the enzymatic hydrolysate, taking a supernatant liquid, adding 1%-10% of HS-15, carrying out activated carbon adsorption at the room temperature, carrying out decarbonization and filtering, regulating the pH value, and filtering after an intermediate is examined to be qualified, so as to obtain the cerebrolysin hydrolysate injection. By virtue of a brand new prescription and process, the prescription safety of the cerebrolysin hydrolysate injection is obviously improved.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, in particular to a cerebroprotein hydrolyzate injection and a preparation method thereof. Background technique [0002] Cerebroprotein hydrolyzate injection is an active peptide hydrolyzate extracted from fresh brain tissue of healthy pigs, containing a variety of amino acids, cephalin, lecithin, and peptide nerve growth factors. It is used to improve symptoms such as insomnia, headache, memory loss, dizziness and irritability, and can promote the rehabilitation of sequelae of traumatic brain injury, sequelae of cerebrovascular disease, sequelae of encephalitis, acute cerebral infarction and acute brain trauma. [0003] Since it went on the market in the 1990s, it has been widely used in the treatment of dementia, cerebrovascular disease and ischemic encephalopathy. It is an economical, safe, small adverse reaction and highly effective brain cell rejuvenating agent, but brain ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/01A61K35/30A61K9/08A61P25/20A61P25/04A61P25/28A61P25/00
Inventor 张浩金红娣陈程梁慧容
Owner CHENGDU LIST PHARMA
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More