Magnetic targeted ultrasound imaging microsphere preparation method

Abstract

The present invention discloses a magnetic targeted ultrasound imaging microsphere preparation method. According to the present invention, the microsphere has a hollow inorganic / organic / inorganic three-layer structure, the cavity is positioned in the center of the microsphere, air is filled in the cavity, the outer layer of the cavity is Fe3O4 magnetic particles, the middle layer is core / shell polymer nanometer microspheres obtained by carrying out free radical copolymerization on a polyethylene-polymethacrylic acid random copolymer, polymethacrylic acid and divinylbenzene, the carboxyl on the inner surface and the iron salt can form the coordination bond or ion bond so as to provide the stability between the copolymer and the Fe3O4 magnetic particles in the inner layer, the outer layer is silicon dioxide, and the carboxyl on the microsphere surface and the silanol on the silicon dioxide surface produce the hydrogen bond effect so as to connect; and the material has the following advantages that: the polymer microspheres belong to the nanoscale particles, the contrast agent can provide the targeted positioning function with the Fe3O4 magnetic nanoparticles in the inner layer, the silicon dioxide hollow microsphere has good biocompatibility and good mechanical stability, and the internal cavity can produce the strong echo signal under the contrasting mode.

Description

technical field [0001] The invention relates to a contrast agent for medical detection, in particular to a multilayer organic / inorganic composite structure nano microsphere with magnetic function, which belongs to the field of medical technology. Background technique [0002] In recent years, molecular imaging technology has attracted widespread attention because it can provide important information for disease diagnosis and monitoring of living tissues (Campbell R.E., Chang C.J.Curr Opin Chem Biol, 2010, 14(1):1-2.). Among many imaging modes, ultrasound imaging, as a non-invasive biomedical imaging mode, has important research value due to its advantages of safe and timely imaging and diagnosis, low price, lightness and quickness, and wide application. However, the difference in echo signals between different biological tissues often hinders the accuracy of diagnosis. In order to better obtain accurate information of specific tissues, people usually use the method of adding...

AI Technical Summary

Problems solved by technology

However, this method still has certain limitations, such as hollow SiO 2 The size of the spheres is too large and does not reach the nanoscale structure, which makes it difficult to be used for in vivo imaging, and it is difficult to carry out further connection without functional group modification

Therefore, the contrast agents currently used in clinical practice cannot simultaneously meet the requirements of good biocompatibility, mechanical stability, targeting performance, nanostructure, and functional group modification.

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