Extraction-separation method and application of corilagin

A separation method and technology for extracting liquid, applied in the field of biomedicine, can solve the problems of insufficient storage and preservation technology, unutilized fruit cores, restrictions on fresh fruit consumption, etc., and achieve the effects of reducing oil-soluble components, being easy to recover, and simplifying processing steps.

Inactive Publication Date: 2015-03-25
GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the concentration of fruit on the market, limited storage and transportation conditions, and insufficient storage and preservation technology, the consumption of fresh fr

Method used

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  • Extraction-separation method and application of corilagin
  • Extraction-separation method and application of corilagin
  • Extraction-separation method and application of corilagin

Examples

Experimental program
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Effect test

Embodiment 1

[0029](1) Extraction of Corilagin: first collect the discarded fruit cores of longan processing in the longan fruit field, dry them naturally, and pulverize them through a 20-mesh sieve to obtain longan fruit core powder. Take 100 g of longan fruit core powder in a 2000 mL three-necked flask, Then add 1500mL of anhydrous acetone, install a stirring device with a seal, plug the other two ports tightly, stir at room temperature for pretreatment for 1h, let stand for 1h, and precipitate the upper layer (basically clear), discard the upper layer; add 1500mL 80% for the second time Acetone aqueous solution, equipped with a thermometer and a reflux device, placed in a water bath to heat and control the solution temperature to 55°C, stirred for 2 hours, then stood still for 1 hour, precipitated the upper layer and filtered it under vacuum at 0.04-0.05MPa to obtain the second filtrate; the third Add 1500mL 60% acetone aqueous solution for the first time, and the operation is the same a...

Embodiment 2

[0037] (1) Extraction of Corilagin: first collect the discarded fruit cores of longan processing in the longan fruit field, dry them naturally, and pulverize them through a 20-mesh sieve to obtain longan fruit core powder. Take 100 g of longan fruit core powder in a 2000 mL three-necked flask, Then add 1500mL of anhydrous acetone, install a stirring device with a seal, plug the other two ports tightly, stir at room temperature for pretreatment for 1h, let stand for 1h, and precipitate the upper layer (basically clear), discard the upper layer; add 1500mL 80% for the second time Acetone aqueous solution, equipped with a thermometer and a reflux device, placed in a water bath to heat and control the solution temperature to 55°C, stirred for 2 hours, then stood still for 1 hour, precipitated the upper layer and filtered it under vacuum at 0.04-0.05MPa to obtain the second filtrate; the third Add 1500mL 60% acetone aqueous solution for the first time, and the operation is the same ...

Embodiment 3

[0045] (1) Extraction of Corilagin: first collect the discarded fruit cores of longan processing in the longan fruit field, dry them naturally, and pulverize them through a 20-mesh sieve to obtain longan fruit core powder. Take 100 g of longan fruit core powder in a 2000 mL three-necked flask, Then add 1500mL of anhydrous acetone, install a stirring device with a seal, plug the other two ports tightly, stir at room temperature for pretreatment for 1h, let stand for 1h, and precipitate the upper layer (basically clear), discard the upper layer; add 1500mL 80% for the second time Acetone aqueous solution, equipped with a thermometer and a reflux device, placed in a water bath to heat and control the solution temperature to 55°C, stirred for 2 hours, then stood still for 1 hour, precipitated the upper layer and filtered it under vacuum at 0.04-0.05MPa to obtain the second filtrate; the third Add 1500mL 60% acetone aqueous solution for the first time, and the operation is the same ...

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Abstract

The invention discloses an extraction-separation method and an application of corilagin. The method comprises the steps of treating longan kernel powder once by using anhydrous acetone and extracting by using acetone aqueous solution again for the second time; uniformly mixing a proper amount of chromatography polyamide with the acetone aqueous solution, recycling acetone and applying to a polyamide chromatography column; eluting by sequentially using pure water, methyl alcohol aqueous solutions with different concentrations and methyl alcohol; gathering 40-60 percent of methyl alcohol eluant in a separate bottle form, condensing and separating out corilagin crystals; mixing mother liquid with the rest of eluant containing corilagin, condensing to be dry, dissolving into methyl alcohol and applying to a polydextran gel LH-20 column; eluting by methyl alcohol; gathering eluant in a separate bottle form and standing; mixing with the eluants in which corilagin crystals are separated out; standing again until the orilagin crystals which are separated out are not increased; mixing the obtained corilagin crystals with corilagin crystals obtained by chromatography polyamide; performing recrystallization by using ethyl alcohol to obtain corilagin with high purity. The corilagin subjected to extraction and separation has a function of inhibiting tyrosinase.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for extracting and separating corilagin and its application. Background technique: [0002] Rangkadilok N et al. reported that dried longan kernels contained 3.7-8.6 mg / g of corilagin; Soong and Barlow determined that longan kernels contained corilagin and gallic acid by using HPLC-ESI-MS technology. , ellagic acid (ellagic acid) and other 13 kinds of phenolic substances. [0003] Corilagin is a galloyl hexahydroxybiphenyl glucopyranoside compound, which belongs to hydrolyzable tannins, soluble in water, methanol, ethanol, acetone and other large polar solvents, and has low toxicity. As early as 1952, Schmidt, O.T. and Lademann, R. discovered that it is a powerful scavenger of free radicals, which is effective for many cardiovascular diseases including hypertension, atherosclerosis, stroke, congestive heart failure, and ischemic heart disease It has a very good ...

Claims

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Application Information

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IPC IPC(8): C07H13/08C07H1/08A61K8/60A61Q19/02
Inventor 郑公铭李忠军刘纲勇邹颖楠王永丽傅中张显策王如意黄海潮付晓春
Owner GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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