Extraction-separation method and application of corilagin

A separation method and technology for extracting liquid, applied in the field of biomedicine, can solve the problems of insufficient storage and preservation technology, unutilized fruit cores, restrictions on fresh fruit consumption, etc., and achieve the effects of reducing oil-soluble components, being easy to recover, and simplifying processing steps.
CN104447896AInactive Publication Date: 2015-03-25GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
Publication Date
2015-03-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an extraction-separation method and an application of corilagin. The method comprises the steps of treating longan kernel powder once by using anhydrous acetone and extracting by using acetone aqueous solution again for the second time; uniformly mixing a proper amount of chromatography polyamide with the acetone aqueous solution, recycling acetone and applying to a polyamide chromatography column; eluting by sequentially using pure water, methyl alcohol aqueous solutions with different concentrations and methyl alcohol; gathering 40-60 percent of methyl alcohol eluant in a separate bottle form, condensing and separating out corilagin crystals; mixing mother liquid with the rest of eluant containing corilagin, condensing to be dry, dissolving into methyl alcohol and applying to a polydextran gel LH-20 column; eluting by methyl alcohol; gathering eluant in a separate bottle form and standing; mixing with the eluants in which corilagin crystals are separated out; standing again until the orilagin crystals which are separated out are not increased; mixing the obtained corilagin crystals with corilagin crystals obtained by chromatography polyamide; performing recrystallization by using ethyl alcohol to obtain corilagin with high purity. The corilagin subjected to extraction and separation has a function of inhibiting tyrosinase.
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Description

Technical field:

[0001] The invention belongs to the field of biomedicine, and in particular relates to a method for extracting and separating corilagin and its application. Background technique:

[0002] Rangkadilok N et al. reported that dried longan kernels contained 3.7-8.6 mg / g of corilagin; Soong and Barlow determined that longan kernels contained corilagin and gallic acid by using HPLC-ESI-MS technology. , ellagic acid (ellagic acid) and other 13 kinds of phenolic substances.

[0003] Corilagin is a galloyl hexahydroxybiphenyl glucopyranoside compound, which belongs to hydrolyzable tannins, soluble in water, methanol, ethanol, acetone and other large polar solvents, and has low toxicity. As early as 1952, Schmidt, O.T. and Lademann, R. discovered that it is a powerful scavenger of free radicals, which is effective for many cardiovascular diseases including hypertension, atherosclerosis, stroke, congestive heart failure, and ischemic heart disease It has a very good ...

Claims

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