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Single polysaccharide component PKP-E-2-2 of pinecone of pinus koraiensis and preparation method of single polysaccharide component PKP-E-2-2

A polysaccharide and Korean pine technology, applied in food preparation, drug combination, anti-toxic agent, etc., can solve the problems of less medicinal development, separation and purification of Korean pine cone polysaccharide and its structural characterization, waste of resources, etc.

Inactive Publication Date: 2015-03-25
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Korean pine is the main tree species in Northeast China. It is widely distributed and has a high yield of pine cones. However, pine cones have always been discarded only as firewood, and their medicinal uses have been rarely developed, resulting in a great waste of resources.
In the past, the research on polysaccharides from Korean pine cones mainly focused on the extraction and separation of crude polysaccharides and the research on biological activity, but the research on the separation, purification and structural characterization of Korean pine cone polysaccharides has not been reported yet.

Method used

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  • Single polysaccharide component PKP-E-2-2 of pinecone of pinus koraiensis and preparation method of single polysaccharide component PKP-E-2-2

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specific Embodiment approach 1

[0052] Specific embodiment 1: The composition of the single polysaccharide component PKP-E-2-2 of the Korean pine cone of this embodiment is composed of L-rhamnose, L-arabinose, D-mannose, D-glucose and D-half Lactose composition;

[0053] The molar ratio of L-rhamnose to L-arabinose is 1.08:2.18; the molar ratio of L-rhamnose to D-mannose is 1.08:2.70; the molar ratio of L-rhamnose The molar ratio to D-glucose is 1.08:0.76; the molar ratio of L-rhamnose to D-galactose is 1.08:2.88;

[0054] The configuration of the single polysaccharide component PKP-E-2-2 of Pinus koraiensis is composed of α-pyranose and β-pyranose;

[0055] The bond link of the single polysaccharide component PKP-E-2-2 of Korean pine cone is mainly linked by 1→4 glycosidic bond or 1→4,6 glycosidic bond, and there are 1→6 glycosidic bond and 1→glycoside Bond, 1→2 glycosidic bond, 1→2,6 glycosidic bond, 1→3 glycosidic bond, 1→3,6 glycosidic bond, 1→2,3 glycosidic bond, 1→2,4 glycosidic bond, 1→3, 4 glycosidic bon...

specific Embodiment approach 2

[0059] Embodiment 2: The method for preparing the single polysaccharide component PKP-E-2-2 of Korean pine cone of this embodiment is carried out according to the following steps:

[0060] 1. Degreasing the Korean pine cone: ① Immerse the Korean pine cone particles in ethanol and extract for 1.5h~2.5h at a temperature of 50~60℃ to obtain the alcohol extract, and then filter out the alcohol extract; ②Repeat ① Operate 2 to 4 times to obtain the degreased Korean pine cone;

[0061] The volume ratio of Korean pine cone to ethanol mentioned in step 1 ① is 1: (4-6);

[0062] The volume concentration of ethanol mentioned in step 1 ① is 94%-96%;

[0063] 2. Hot water extraction: ① Immerse the defatted Korean pine cone obtained in step 1 in water, extract for 1.5h~2.5h at a temperature of 95℃~105℃, repeat the extraction 2~4 times, filter and collect Each time of the filtrate, the water extract is obtained; ②The water extract obtained in step 2 ① is concentrated to a relative density of 45% to...

specific Embodiment approach 3

[0090] Specific embodiment three: This embodiment is different from specific embodiment two in that in step one (1), the Korean pine cone particles are immersed in ethanol, and extracted at a temperature of 55° C. for 2 hours to obtain an alcohol extract. The other steps and parameters are the same as in the second embodiment.

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Abstract

The invention discloses a single polysaccharide component PKP-E-2-2 of a pinecone of pinus koraiensis and a preparation method of the single polysaccharide component PKP-E-2-2, relates to a single polysaccharide component of the pinecone of the pinus koraiensis and a preparation method of the single polysaccharide component, particularly relates to a single polysaccharide component PKP-E-2-2 of the pinecone of the pinus koraiensis and a preparation method of the single polysaccharide component PKP-E-2-2, and aims at solving the problem of shortage of research in separation and purification of polysaccharides of the pinecone of the pinus koraiensis and structural characterization at present. The component PKP-E-2-2 is prepared from the following raw materials: L-rhamnose, L-gum sugar, D-mannose, D-glucose and D-galactose; and the configuration is formed by alpha-pyranose and beta-pyranose. The method comprises the following steps: 1, degreasing the pinecone of the pinus koraiensis; 2, leaching in hot water; 3, deproteinizing with water extract liquid; 4, carrying out alcohol precipitation on crude polysaccharides with ethyl alcohol; 5, carrying out grading-alcoholic precipitation on the crude polysaccharides; 6, carrying out freeze drying; and 7, separating and purifying.

Description

Technical field [0001] The invention relates to a single polysaccharide component of Korean pine cone and a preparation method thereof, and in particular to a single polysaccharide component of Korean pine cone PKP-E-2-2 and a preparation method thereof. Background technique [0002] Red pine (Pinus Koraiensis) belongs to the Pinus family, also known as fruit pine. Korean pine is only distributed in the area from Xiaoxing'anling to Changbai Mountain in northeast China, and only in parts of Russia, Japan, and North Korea. The natural conditions in the Xiaoxinganling area in my country are the most suitable for the growth of red pine. More than half of the world's red pine resources are distributed here. Yichun is known as the "hometown of red pine". Red pine is cold-resistant, prefers slightly acidic or neutral soil, the wood is soft, contains rosin, and has strong antiseptic properties, so it has a wide range of uses; needles and cones can extract antioxidant active ingredients;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06A23L1/09A23L33/125
Inventor 邹攀杨鑫赵海田
Owner HARBIN INST OF TECH
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